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  • 1
    Publication Date: 2023-10-26
    Description: 〈title xmlns:mml="http://www.w3.org/1998/Math/MathML"〉Abstract〈/title〉〈p xmlns:mml="http://www.w3.org/1998/Math/MathML" xml:lang="en"〉Atmospheric gravity waves play an important role in driving the dynamics of the Mesosphere and Lower Thermosphere and the basic structure of this region is determined by momentum deposition of these waves. Mesospheric bores are a type of non‐linear response that cause the amplification of gravity wave, due to trapping, that is characterized by a propagating step‐like jump followed by undulating waves. They require a stable layer or duct to travel horizontally with little attenuation thereby capable of transporting wave energy and momentum over larger distances. We present a prominent bright undular bore event observed in the mesospheric O(〈sup〉1〈/sup〉S), O〈sub〉2〈/sub〉, and OH emission layers on 16 March 2021 over Germany. A striking feature of this observation is the capture of bore's rapid dissipation around the center of the imager's field of view. The vertical temperature profile obtained from the satellite data indicates the presence of temperature inversion layer which acted as a thermal duct for the bore propagation. In addition, we have performed idealized two dimensional direct numerical simulations (DNS) of Navier‐Stokes equations under Boussinesq approximation. The DNS results reproduce many important characteristics of the observed airglow event like the nonlinear wave‐steepening, number of trailing waves, and its dissipation by implementing a thermal duct and a wave‐like perturbation. Furthermore, the DNS results also indicate that the duct width and amplitude of the initial perturbation have a considerable effect on the bore morphology.〈/p〉
    Description: Key Points: 〈list list-type="bullet"〉 〈list-item〉 〈p xml:lang="en"〉Observation of a mesospheric bright bore event that dissipated within the field of view〈/p〉〈/list-item〉 〈list-item〉 〈p xml:lang="en"〉The duct that enabled the bore propagation was near the O(〈sup〉1〈/sup〉S) emission layer based on the observational data〈/p〉〈/list-item〉 〈list-item〉 〈p xml:lang="en"〉The majority of the observed features are reproduced with idealized 2D direct numerical simulations using Boussinesq approximation〈/p〉〈/list-item〉 〈/list〉 〈/p〉
    Description: Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
    Description: Alexander von Humboldt‐Stiftung http://dx.doi.org/10.13039/100005156
    Description: https://doi.org/10.22000/809
    Description: http://sirius.bu.edu/data/
    Description: http://saber.gats-inc.com/coin.php
    Keywords: ddc:551.5 ; bores ; direct numerical simulations ; gravity waves ; inversion layers
    Language: English
    Type: doc-type:article
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  • 2
    Keywords: Cognition. ; Creative thinking. ; Imagery (Psychology) ; Visualization.
    Pages: vi, 239 p.
    Edition: 1st pbk. ed
    ISBN: 0-585-03104-5
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  • 3
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The genome of Arabidopsis thaliana contains six putative cell-wall type invertase genes (AtcwINV1-6). Heterologous expression of AtcwINV1, 3 and 6 cDNAs in Pichia pastoris revealed that the enzymes encoded by AtcwINV3 and 6 did not show invertase activity. Instead, AtcwINV3 is a 6-FEH and AtcwINV6 is a fructan exohydrolase (FEH) that can degrade both inulin and levan-type fructans. For AtcwINV6 it is proposed to use the term (6&1) FEH. In contrast, AtcwINV1 is a typical invertase. FEH activity was also detected in crude extracts of different parts of Arabidopsis. To verify that the FEH activity of AtcwINV3 and 6 were not artefacts of the heterologous expression system, the protein corresponding to AtcwINV3 was isolated from whole Arabidopsis plants and indeed showed only 6-FEH activity and no invertase activity. Although no fructans can be detected in Arabidopsis plants, it is shown that kestoses (trimers) can be synthesized in crude leaf extracts. The putative physiological significance of FEH in so-called non-fructan plants is discussed.
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  • 4
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The apoaequorin-coding region from complementary DNA clone pAEQl (ref. 7) was fused to the cauliflower mosaic virus (CMV) 35S promoter8 and transferred to Nicotiana plum-baginifolia using the Agrobacterium tumefaciens pBIN19 binary vector system9 to provide constitutive expression (Fig. la). ...
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  • 5
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Polyadenylated RNA from leaves of pea (Pisum sativum) has been copied into DNA and cloned in the Escherichia coli plasmid pBR322. From these clones we have identified and sequenced DNA encoding the mRNA for the precursor of the small subunit of the chloroplast enzyme ribulose-1,5-bisphosphate ...
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  • 6
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 278 (1979), S. 662-664 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Isolated pea chloroplasts synthesise as the major soluble product the large subunit of ribulose bisphosphate carboxy-lase/oxygenase, the key enzyme in photosynthesis and photo-respiration3. The carboxylase enzyme (RBPCase) is an oligomer of 16 subunits. Besides eight large catalytic subunits, the ...
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  • 7
    ISSN: 1432-2048
    Keywords: Cucumis (cotyledon) ; Glyoxylate cycle ; Senescence ; Sucrose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When cotyledons are detached from cucumber (Cucumis sativus L. cv. Masterpiece) plants 14 d after seed imbibition, accumulation of the glyoxylate-cycle enzymes malate synthase (MS) and isocitrate lyase (ICL) occurs rapidly in the dark but not in the light. This is primarily due to an increase in transcript abundance. It has been proposed that glyoxylate-cycle enzymes are synthesised in response to lipid degradation. However, MS and ICL synthesis in detached cucumber cotyledons begins before a decline in lipid can be detected. Furthermore, the activation of MS and ICL gene expression does not correlate with loss of chlorophyll, carotenoid, protein or RNA. These results are discussed in terms of the activation of genes encoding glyoxylate-cycle enzymes and their possible function in detached and senescing organs. Previous work (Graham et al, 1992, Plant Cell 3, 349–357) has suggested that sucrose or other carbohydrates can repress the synthesis of MS and ICL. Consistent with this proposal, incubation of detached cotyledons in the light leads to a fivefold increase in sucrose content. However, when MS and ICL synthesis occurs in the dark, no change in the amount of sucrose is detected. Incubation of cotyledons in the dark in the presence of 25 mM sucrose leads to a complete inhibition of the accumulation of MS and ICL. Similarly, cotyledon protoplasts incubated in darkness accumulate MS and ICL, but not if 25 mM sucrose is included in the medium. These results collectively support the view that MS and ICL synthesis responds to the metabolic activities of plant cells.
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  • 8
    ISSN: 1432-2048
    Keywords: Key words: Antisense inhibition ; D-enzyme ; Gluca‐notransferase ; Solanum tuberosum ; Starch meta-bolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Transgenic potato (Solanum tuberosum L.) plants were created with sense and antisense copies of the potato D-enzyme (disproportionating enzyme; EC␣2.4.1.25) cDNA linked to patatin and cauliflower mosaic virus 35 S promoters, and screened for D-enzyme activity in tubers. Transformants with sense constructs mostly had wild type D-enzyme activity but two plants had only about 1% wild-type activity. Transformants with antisense constructs had activity ranging from 90% to about 1% of wild type. Three 35 S antisense plants with very low activity were analysed in detail. Western blot analysis showed that D-enzyme was present in greatly reduced amounts in tubers and in leaves, whereas plastidic starch phosphorylase (EC 2.4.1.1) was unaffected. The lack of D-enzyme resulted in slow plant growth but development was otherwise apparently normal. Furthermore, the starch content of tubers was not appreciably altered in amount, proportion of amylose, molecular weight of debranched amylopectin, or branch chain length, despite the lack of D-enzyme. These results do not indicate a direct requirement for D-enzyme in the synthesis and accumulation of storage starch in tubers. The results are discussed in terms of the known reactions catalysed by D-enzyme and possible involvement of D-enzyme in starch metabolism.
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  • 9
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The extent of methylation of the 18S–25S ribosomal RNA genes ofPetunia hybrida has been investigated using methylation-sensitive restriction endonucleases and Southern hybridisation. The genes in leaves, ovaries, petals, stigmas, anthers, and seed are methylated to a similar extent, but those in roots are hypomethylated. Genes in adventitious roots formed from stem cuttings are also hypomethylated. Genes in callus cultures initiated from leaf discs vary widely in the extent of methylation, but the pattern obtained is stable during culture. Callus cells containing hypomethylated genes can give rise to shoots in which the extent of methylation has reverted to that of the original leaf explant. These results demonstrate the striking plasticity of ribosomal RNA gene methylation inPetunia.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 26 (1994), S. 423-434 
    ISSN: 1573-5028
    Keywords: cucumber ; Cucumis sativus ; germination ; gluconeogenesis ; phosphoenolpyruvate carboxykinase ; senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA library from RNA of senescing cucumber cotyledons was screened for sequences also expressed in cotyledons during post-germinative growth. One clone encodes ATP-dependent phosphoenolpyruvate carboxykinase (PCK; EC 4.1.1.49), an enzyme of the gluconeogenic pathway. The sequence of a fulllength cDNA predicts a polypeptide of 74397 Da which is 43%, 49% and 57% identical to bacterial, trypanosome and yeast enzymes, respectively. The cDNA was expressed in Escherichia coli and antibodies raised against the resultant protein. The antibody recognises a single polypeptide of ca. 74 kDa, in extracts of cotyledons, leaves and roots. The cucumber genome contains a single pck gene. In the seven-day period after seed imbibition, PCK mRNA and protein steady-state levels increase in amount in cotyledons, peaking at days 2 and 3 respectively, and then decrease. Both accumulate again to a low level in senescing cotyledons. This pattern of gene expression is similar to that of isocitrate lyase (ICL) and malate synthase (MS). When green cotyledons are detached from seedlings and incubated in the dark, ICL and MS mRNAs increase rapidly in amount but PCK mRNA does not. Therefore it seems unlikely that the glyoxylate cycle serves primarily a gluconeogenic role in starved (detached) cotyledons, in contrast to post-germinative and senescing cotyledons where PCK, ICL and MS are coordinately synthesised. While exogenous sucrose greatly represses expression of icl and ms genes in dark-incubated cotyledons, it has a smaller effect on the level of PCK mRNA.
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