ALBERT

Notes: During lysogenic growth many temperate bacteriophage genomes are integrated into the host's chromosome and efficient integration and excision are therefore an essential part of the phage life cycle. The Streptomyces phage φC31 encodes an integrase related to the resolvase/invertases and is evolutionarily and mechanistically distinct from the integrase of phage λ. We show that during φC31 integration the polarity of the recombination sites, attB and attP, is dependent on the sequences of the two base pairs (bp) where crossover occurs. A loss or switch in polarity of the recombination sites can occur by mutation of this dinucleotide, leading to incorrectly joined products. The properties of the mutant sites implies that φC31 integrase interacts symmetrically with the substrates, which during synapsis can align apparently freely in either of two alternative forms that lead to correct or incorrect joining of products. Analysis of the topologies of the reaction products provided evidence that integrase can synapse and activate strand exchange even when recombinant products cannot form due to mismatches at the crossover site. The topologies of the recombination products are complex and indicative of multiple pathways to product formation. The efficiency of integration of a φC31 derivative, KC859, into an attB site with switched polarity was assayed in vivo and shown to be no different from integration into a wild-type attB. Thus neither the host nor KC859 express a factor that influences the alignment of the recombination sites at synapsis.

Notes: Abstract Deep sea photographs were collected for several camera-tow transects along and across the axis at the East Pacific Rise crest between 9°49′ and 9°52′ N, covering terrain out to 2 km from the ridge axis. The objective of the surveys was to utilize fine-scale morphology and imagery of seafloor volcanic terrain to aid in interpreting eruptive history and lava emplacement processes along this fast-spreading mid-ocean ridge. The area surveyed corresponds to the region over which seismic layer 2A, believed to correspond to the extrusive oceanic layer, attains full thickness (Christeson et al., 1994a, b, 1996; Hooft et al., 1996; Carbotte et al., 1997). The photographic data are used to identify the different eruptive styles occurring along the ridge crest, map the distribution of the different morphologies, constrain the relative proportions of the three main morphologies and discuss the implications of these results. Morphologic distributions of lava for the area investigated are 66% lobate lava, 20% sheet lava, 10% pillow lava, and 4% transitional morphologies between the other three main types. There are variations in inferred relative lava ages among the different morphological types that do not conform to a simple increase in age versus distance relationship from the spreading axis, suggesting a model in which off-axis transport and volcanism contribute to the accumulation of the extrusive layer. Analysis of the data suggests this ridge crest has experienced three distinctly different types of volcanic emplacement processes: (1) axial summit eruptions within a ∼1 km wide zone centered on the axial summit collapse trough (ASCT); (2) off-axis transport of lava erupted at or near the ASCT through channelized surface flows; and (3) off-axis eruptions and local constructional volcanism at distances of ∼0.5-1.5 km from the axis. Major element analyses of basaltic glasses from lavas collected by Alvin, rock corer and dredging in this area indicate that the most recent magmatic event associated with the present ASCT erupted relatively homogeneous and mafic (〉8.25 weight percent wt.% MgO) basalts compared to older, off-axis lavas which tend to be more chemically evolved (Perfit and Chadwick, 1998; Perfit and Fornari, unpublished data). The more primitive lavas have a more extensive distribution within and east of the ASCT. More evolved basalts (MgO 〈8.0wt.%) are concentrated in a broad area a few kilometers east of the axis, and in an oval-shaped area south of 9°50′ N, west of the ASCT. Transitional and enriched (T- and E-) mid-ocean ridge basalts exist in relatively small areas (〈1 km2) on the crestal plateau and correlate with scarps or fissures where pillow lavas were erupted. Mafic lavas in this area are primarily related to the youngest magmatic events. Geochemical analysis of samples collected at distances 〉∼500 m from the ASCT suggests that regions of off-axis volcanism may be sourced from older and cooler sections of the axial magma lens. Analysis of these data suggests that this portion of the EPR has not experienced large scale volcanic overprinting in the past ∼30 ka. The predominance of lobate flows (66%) throughout much of the crestal region, and subtle variations in sediment cover and apparent age between flows, suggest that eruptive volumes and effusion rates of individual eruptions have been similar over much of the last 30 ka and that most of the eruptions have been small, probably similar in volume to the 1991 EPR flow which had an estimated volume of ∼1×106 m3 (Gregg et al., 1996).