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  • 1
    ISSN: 1432-0983
    Keywords: Key words Cold sensitivity ; Exonuclease II ; Microtubule inhibitors ; Strand-exchange protein Sep1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Exonuclease II (ExoII) from Schizosaccharomyces pombe is a 5′→3′ single-stranded DNA exonuclease. We have cloned its gene, exo2, whose nucleotide sequence revealed that ExoII is a homologue of the multifunctional Saccharomyces cerevisiae Sep1 protein (also called Kem1, Xrn1, Rar5, Dst2). S. pombe exo2 null mutants were cold-sensitive for growth, had increased cell size at the restrictive temperature, were hypersensitive to the mitotic inhibitor thiabendazol and to caffeine, and died rapidly in stationary phase. Many of these phenotypes are similar to those of sep1 (kem1 or xrn1) mutants of S. cerevisiae. In contrast, the exo2 mutation had only a moderate effect on progression through meiosis and no significant effect on meiotic recombination. We discuss possible functions of the multifunctional ExoII protein.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A sensitive, specific polymerase chain reaction-based assay was developed for the detection of the causal agent of ratoon stunting disease of sugarcane, Clavibacter xyli subsp. xyli. This assay uses oligonucleotide primers derived from the internal transcribed spacer region between the 16S and 23S rRNA genes of the bacterial rRNA operon. The assay is specific for C. xyli subsp. xyli and does not produce an amplification product from the template of the closely related bacterium C. xyli subsp. cynodontis, nor from other bacterial species. The assay was successfully applied to the detection of C. xyli subsp. xyli in fibrovascular fluid extracted from sugarcane and was sensitive to approximately 22 cells per PCR assay. A multiplex PCR test was also developed which identified and differentiated C. xyli subsp. xyli and C. xyli subsp. cynodontis in a single PCR assay.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Angular distributions of the differential cross section for positive pion absorption on the deuteron have been measured at 170, 208 and 285 MeV. The data have been fitted with Legendre polynomials. Along with other recent experiments, these data are compared with recent theoretical descriptions.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 50 (1994), S. 234-241 
    ISSN: 1420-9071
    Keywords: Homologous recombination ; hotspots ; nucleases ; meiosis ; Escherichia coli ; Chi ; Schizosaccharomyces pombe ; M26
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Homologous recombination occurs at higher than average frequency at and near hotspots. Hotspots are special nucleotide sequences recognized by proteins that promote, directly or indirectly, a rate limiting step of recombination. This review focuses on two well-studied examples, the Chi sites of the bacteriumEscherichia coli and the M26 site of the fission yeastSchizosaccharomyces pombe. Chi, 5′ G-C-T-G-G-T-G-G 3′, is recognized by the RecBCD enzyme, which nicks the DNA near Chi and produces a 3′-ended single-stranded DNA ‘tail’; this tail is a potent substrate for homologous pairing by RecA and single-stranded DNA binding proteins. M26, 5′ A-T-G-A-C-G-T 3′, is recognized by a heterodimeric protein and stimulates, by an as-yet-unknown mechanism, meiotic recombination at and near theade6 gene. Additional hotspots in bacteria, fungi, and mammals enhance recombination directly or indirectly via a variety of mechanisms. Although hotspots are widespread among organisms, the biological role of their localized enhancement of recombination remains a matter of speculation.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1617-4623
    Keywords: Key words Meiosis ; Recombination ; Replication ; Transcript induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Schizosaccharomyces pombe rec16-125 mutation reduces meiotic recombination, delays pre-meiotic DNA synthesis, and reduces the accumulation of some but not other rec gene transcripts. To elucidate the function of the Rec16 global meiotic regulator, we cloned and sequenced rec16. The data revealed that rec16 is identical to rep1, which was previously shown to encode a protein with a zinc-finger motif required for pre-meiotic DNA synthesis. Transcripts of rec16 (rep1) were strongly induced and subsequently degraded during meiosis. In a rec16 (rep1) deletion mutant, meiotic induction of the seven rec genes tested, which appear to be directly involved in meiotic recombination, was significantly reduced or essentially abolished. Deletion of 80% of the gene essentially abolished meiotic recombination, whereas strains deleted for approximately one-quarter of the gene, from either end, retained partial activity. The rec16-125 mutation strongly reduced recombination in the intervals tested on chromosomes I and III, a phenotype characteristic of mutations in rec genes, such as rec7, whose expression requires Rec16 (Rep1). These results show that Rec16 (Rep1) does not have the regional specificity of Rec10. We infer that Rec16 (Rep1) is a transcriptional activator that is required for meiotic replication and recombination because it plays a role in the transcriptional induction of the rec and other meiosis-specific genes.
    Type of Medium: Electronic Resource
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  • 6
    Publication Date: 2013-08-31
    Description: Visible near-infrared sensors mounted on operational satellites now in use do not have on-board full aperture absolute calibration devices. One means of establishing an in-orbit calibration for a satellite sensor is to make simultaneous measurements of a bright, uniform scene along the satellite view vector from a calibrated instrument on board a high altitude aircraft. In the work reported here, aircraft data were recorded over White Sands, New Mexico at satellite overpass time for the LANDSAT-5 Thematic Mapper (TM). A comparison of the coincident aircraft and orbiting satellite data showed the radiometric gain for TM channel 1 had degraded 4.7 percent by August 28, 1985; gains for TM channels 2 and 3 were within 1 percent of prelaunch values.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: A-89107 , NASA-TM-102185 , NAS 1.15:102185
    Format: application/pdf
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  • 7
    Publication Date: 2013-08-31
    Description: The interactive signal analysis and ultrasonic data collection system (ECHO1) is a real time data acquisition and display system. ECHO1 executed on a PDP-11/45 computer under the RT11 real time operating system. Extensive operator interaction provided the requisite parameters to the data collection, calculation, and data modules. Data were acquired in real time from a pulse echo ultrasonic system using a Biomation Model 8100 transient recorder. The data consisted of 2084 intensity values representing the amplitude of pulses transmitted and received by the ultrasonic unit.
    Keywords: COMPUTER PROGRAMMING AND SOFTWARE
    Type: NASA-CR-150745 , REPT-78-066
    Format: application/pdf
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  • 8
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 110 (1988), S. 609-610 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 1. Bluegill sunfish (Lepomis macrochirus) dominate fish assemblages of small lakes and ponds throughout the eastern United States and may play a major role in structuring aquatic communities. We examined the impact of adult bluegill on amphibian density by stocking bluegill at a range of densities into partitions of an experimental pond in which amphibians were free to colonize.2. Adult bluegill had a major impact on the amphibian assemblage. By the end of the experiment, gray treefrog (Hyla versicolor) tadpoles were nine times less abundant, and red-spotted newt (Notophthalmus viridescens) adults were three times less abundant in the presence of adult bluegill than in their absence. In contrast, bullfrog (Rana catesbeiana) tadpoles tended to increase in the presence of adult bluegill. Adult bluegill also had a negative effect on the abundance of predaceous aquatic insects.3. There was no indication that interactions among amphibians were significant in determining the above patterns. We suggest that the strong impact of adult bluegill resulted from a combination of direct and indirect effects on amphibian larvae and predaceous aquatic insects.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1573-5036
    Keywords: arrowleaf clover ; labelled N ; Lolium multiflorum ; 15N depleted ammonium nitrate ; N transfer ; ryegrass ; Trifolium vesiculosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Arrowleaf clover (Trifolium vesiculosum Savi) and annual ryegrass Lolium multiflorum Lam.) commonly are overseeded in dormant bermudagrass (Cynodon dactylon L. Pers.) sod on coastal plain soils in the southeastern United States. Two field experiments were conducted in consecutive years at different sites to estimate the amount of N transferred from the clover to the annual grass. Nitrogen treatments included 50 kg N ha-1 as 15N depleted ammonium nitrate applied in either February or April, and a check (no N applied). Three clippings were made during the cool-season from March to June. In both experiments, less than 5 kg N ha-1 were transferred from the clover to the grass. Ryegrass yields of dry matter and total N were not increased by growing with clover. Clover growth was typical for the region; average dry matter yield in pure stand was 2,615 kg ha-1 over the two-year period. Clover in mixed stand fixed between 20 and 60 kg N/ha. Less than 13% of N contained in ryegrass was transferred from arrowleaf clover to ryegrass at any clipping while clover was actively growing. The quantity of N transferred over the entire season was not statistically significant.
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