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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Plant pathology 45 (1996), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Red leaf blotch of soybeans, caused by Dactuliochaeta glycines, was evaluated on soybean plants in field plots located in Zambia. Two experiments were conducted in each of two seasons. Experiment 1 had four cultivars that were either fungicide-sprayed or not sprayed. Disease severity was greatest on leaves at the lowest nodes from early vegetative through the reproductive growth stages. Area under the disease progress curve (AUDPC) values and percentage of nodes defoliated at growth stage R5 were significantly (P = 0.05) greater in unsprayed plots for all cultivars in both seasons. Yield losses ranged from 8 to 37% while reduced seed size ranged from 21 to 29% for the four cultivars. Number of pods per plant in fungicide-sprayed plots did not differ from those in unsprayed plots. However, the number of seeds per plant and seeds per pod were significantly (P = 0.05) greater in sprayed than unsprayed plots for some cultivars. In experiment 2, cultivar Tunia was either fungicide-sprayed at different times or not sprayed. The lowest attached leaf had the most variation in the amount of disease while ratings of the most median leaf in the canopy were generally less variable. The AUDPC values calculated from the lowest attached leaf, the mean of all attached leaves, and the median attached leaf differed significantly (P = 0.05) the number of times plants were sprayed with fungicide. Defoliation and vertical incidence of red leaf blotch from lower to higher nodes were significantly (P = 0.05) reduced in fungicide-sprayed plots in one season, but not the other. One thousand-seed weight and yield differed significantly (P = 0.05) with treatment as one application of triphenyltin acetate increased yields by 18% over unsprayed plots in season 1. One thousand-seed weight and yield, regressed on the AUDPC for the median leaf in the canopy, explained 92 and 72% of the variation, respectively.
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  • 2
    ISSN: 1573-8248
    Keywords: antagonism ; biocontrol ; chemotaxis ; motility ; soybean ; sporulation ; Bacillus megaterium ; Rhizoctoria solani
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Bacillus megaterium strainB153-2-2 is a potential bacterial biocontrol agentagainst Rhizoctonia solani isolate 2B12(ISG-2B). To study the role of antagonism (Ant),chemotaxis (Che), motility (Mot), and sporulation(Spo) of the biocontrol agent during seed and rootcolonization and the correlation between rootcolonization and the suppression of soybean (Glycine max) root rot caused by R. solani,strain B153-2-2(Che+Mot+Ant++Spo++) and the sevenderived mutants with altered antagonism, chemotaxis,motility, and/or sporulation were used. The bacterialcells were introduced into soil separately either asa soybean seed coating or soil application. Two soilmixtures defined as coarse and fine soil were used. The bacterial cell chemotactic response to soybeanroot and seed exudates and antagonism to R.solani were significantly (p = 0.05) correlatedwith root and seed colonization in some but not alltreatments. The sporulation-defective mutants had lowcell populations immediately after application and,therefore, reduced root colonization. The differencesin root colonization diminished among the mutants andstrain B153-2-2 when R. solani was present inthe soil or, as seedlings grew older. Soybean seedlingroots grown in coarse soil had significantly greatercolonization by B153-2-2 or its mutants and a lowerdisease index than that in fine soil. There was asignificant positive correlation (r 2 = 0.78)between root colonization by strain B153-2-2 or itsmutants and suppression of Rhizoctonia root rot.
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  • 3
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Emergence of ‘Amsoy’ soybean (Glycine max) seed inoculated withSclerotinia sclerotiorum was significantly reduced below noninoculated seed at soil temperatures of 25°, 30°, and 35 °C, but not at 20 °C.S. sclerotiorum was readily reisolated from wound-inoculated stems of seedlings and nearly mature plants above the point of inoculation and below to the crown area, but not from roots. The fungus was recovered from stems but not roots of 15-day seedlings grown in sterile soil before infestation of the soil surface with a suspension of mycelium and sclerotia and assayed at 15 days after soil infestation. When compared to healthy, seeds, infected seeds withS. sclerotiorum were characterized by appearing flattened.
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  • 4
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Emergence of “Amsoy” soybean (Glycine max) seed inoculated withSclerotinia sclerotiorum was significantly reduced below noninoculated seed at soil temperatures of 25, 30 and 35 °C, but not at 20 °C.S. sclerotiorum was readily·reisolated from wound-inoculated stems of seedlings and nearly mature plants above the point of inoculation below to the crown area, but not from roots. The fungus was recovered from stems but not roots of seedlings grown in sterile soil for 15 days before infestation of the soil surface with a suspension of mycelium and sclerotia and assayed at 15 days after soil infestation. When compared to healthy, seed infected withS. sclerotiorum were characterized by appearing flattened.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 43 (1971), S. 309-315 
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A qualitative comparison was made of the genera of fungi associated with creeping bentgrass turf (Agrostris palustris) plots either nonsprayed or sprayed with six fungicides for 4 or 5 successive years. The fungicides used were: Fore (manganese ethylene bisdithiocarbamate plus zinc ion) Actidone-Thiram (3[2-(3, 5-dimethyl-2-oxycyclohexyl)-2-hydroxy-ethyl]) glutarimide plus tetramethyl thiuram disulfide); Dyrene (2, 4-dichloro-6-0-chloroanillino-s-triazine; Kromad plus Calaclor (5% cadmium sebacate, 5% potassium chromate, 1% malachite green, 0.5% auramine, 16c tetramethyl thiuram disulfide plus 60% mercurous chloride, 30% mercuric chloride); PMA plus Thiram (phenylmercuric acetate plus tetramethyl thiuram disulfide); and Tersan OM (hydroxymercuric chlorophenol plus tetramethyl thiuram disulfide). None of the fungicides had any significant effect on the distribution of fungal genera studied.
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  • 6
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A variety of interactions was recorded in culture between 11 saprobic fungi isolated from soybean (Glycine max) roots and seeds and the soybean pathogens Cercospora sojina, Colletotrichum truncatum, Macrophomina phaseolina, Phomopsis sojae, and Septoria glycines. The most active saprobes were Aspergillus terreus, Chaetomium cupreum, Epicoccum nigrum, Gliocladium roseum, Myrothecium roridum, Penicillium thomii, and Trichothecium roseum. Hyphal lysis of several fungal pathogens by Acremonium sp., C. cupreum and P. thomii was recorded perhaps because of parasitism by G. roseum and T. roseum. In greenhouse studies, seeds coated with G. roseum, P. thomii, and T. harzianum emerged significantly (P=0.05) more than those coated with A. terreus and the control. In field studies, seeds coated with a conidial suspension of A. terreus, G. roseum, P. thomii or Trichoderma harzianum produced a significantly greater stand than the control. The area of cotyledons covered with lesions caused by C. truncatum was significantly less on seeds coated with G. roseum, P. thomii and T. harzianum than the control.
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  • 7
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Filter-sterile culture filtrates of an isolate of Diaporthe phaseolorum var. sojae (Phomopsis sojae), causal agent of pod and stem blight and seed decay of soybeans (Glycine max), grown on Czapeks-dox broth significantly (P = 0.05) inhibited germination of cabbage (Brassica oleracea), cantaloupe (Cucumis melo), onion (Allium cepa), soybean, and wheat (Triticum vulgare) seeds and wilted soybean seedling cuttings within 24 hr. Inhibition of seed germination and severity of soybean seedling wilt increased with increased concentrations of culture filtrates and increased incubation time of the cultures.
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  • 8
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Filter-sterile culture filtrates of isolates of Diaporthe phaseolorum var. caulivora, Diaporthe phaseolorum var. sojae (anamorph Phomopsis sojae), and Phomopsis longicolla, causal agents of stem canker, pod and stem blight, and seed decay, respectively, on soybeans (Glycine max), grown on Czapek-Dox broth inhibited germination of soybean seeds (significant at P=0.05). Wilting and necrosis of excised soybean seedlings was significantly greater than the controls when treated with culture filtrates of P. longicolla and P. sojae. Soybean seedling radicle growth inhibited significantly when culture filtrates of P. longicolla were added.
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  • 9
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The antagonism between Gliodadium roseum, Trichoderma harzianum, or Trichothecium roseum and Phytophthora megasperam f. sp. glycinea (Pmg), cause of Phytophthora rot of soybeans (Glycine max), was studied. G. roseum, T. harzianum, and 17 isolates of T. roseum were grown separately on modified Czapek-Dox medium (MCD) in the dark for 25 days at 25 °C. Culture filtrates of T. roseum at 0.5% and 20.0% concentrations inhibited mycelial growth of Pmg at 3.1% and 90.4%; and those of G. roseum at −0.7% and 44.0%; and of T. harzianum at 0.7% and 46.0%, respectively. Culture filtrates of T. roseum at 0.5% concentration inhibited zoosporangenesis of Pmg at 98.0% and at 1.0% concentration prevented it. Culture filtrates of G. roseum and T. harzianum at 5% concentration significantly (P=0.05) inhibited zoosporangenesis of Pmg, but differences were not significant from that on MCD. Culture filtrates of 17 isolates of T. roseum inhibited mycelial growth and zoosporangenesis of Pmg at different percentages with different concentrations with those of isolate 9 showing the greatest inhibition of both. Mycelial growth of most of 16 races of Pmg was prevented at 10% concentration of the culture filtrates of isolate SS-2 of T. roseum, and all 16 races, except race 6, was prevented at 20% concentration. Zoosporangenesis of all races of Pmg was prevented at 2% the culture filtrate of SS-2. Culture filtrates of SS-2 inhibited zoosporangenesis of Pmg in soil.
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  • 10
    Publication Date: 1989-03-01
    Print ISSN: 0002-1962
    Electronic ISSN: 1435-0645
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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