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  • 1
    ISSN: 1573-8221
    Keywords: macrophages ; brucellas ; antibodies ; phagocytosis ; pinocytosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The ingestion of a heterologous brucellar luminescent antiserum by cultures of macrophages from intact and immune guinea pigs and its accumulation in the cytoplasmic granules (secondary lysosomes) and its entry into phagocytic vacuoles (phagosomes) were studied. The accumulation of antiserum in the phagosomes was judged from the appearance of specific luminescence of the phagocytosed brucellas. Accumulation of luminescent antigen-antibody complex in the cytoplasmic granules also was investigated at various time intervals after phagocytosis of brucellas treated with brucellar luminescent serum. No difference was found between macrophages from intact and immune animals as regards the character of pinocytosis of the luminescent sera. Differences between macrophages were found in the rate of passage of the antisera from the lysosomes into the phagosomes and the rate of formation of luminescent granules after phagocytosis of brucellas treated with luminescent antiserum.
    Type of Medium: Electronic Resource
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