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  • 1
    Electronic Resource
    Electronic Resource
    Effects of dissolved humic substances on the speciation of iron and phosphate at different pH and ionic strength (1993)
    s.l. : American Chemical Society
    Environmental science & technology 27 (1993), S. 1052-1059 
    Details
    ISSN: 1520-5851
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/es00043a003
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  • 2
    Electronic Resource
    Electronic Resource
    The influence of humic substances on the molecular weight distributions of phosphate and iron in epilimnetic lake waters (2000)
    Oxford, UK : Blackwell Science Ltd
    Freshwater biology 45 (2000), S. 0 
    Details
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 1. The influence of humic substances on the association of free inorganic iron and phosphate with material of larger molecular weight was investigated in epilimnetic samples from two humus-rich lakes of contrasting ionic strength. After modification of the molecular weight distribution of the humic substances in the samples using dialysis and ultrafiltration, the molecular weight distribution of added radioisotopes ( 〈inlineGraphic alt="inline image" href="urn:x-wiley:00465070:FWB634:FWB_634_mu1" location="equation/FWB_634_mu1.gif" extraInfo="missing"/〉 Fe3+ and 〈inlineGraphic alt="inline image" href="urn:x-wiley:00465070:FWB634:FWB_634_mu2" location="equation/FWB_634_mu2.gif" extraInfo="missing"/〉 PO43−) was assessed using gel filtration chromatography. 
2. The association of 〈inlineGraphic alt="inline image" href="urn:x-wiley:00465070:FWB634:FWB_634_mu3" location="equation/FWB_634_mu3.gif" extraInfo="missing"/〉 Fe3+ and 〈inlineGraphic alt="inline image" href="urn:x-wiley:00465070:FWB634:FWB_634_mu4" location="equation/FWB_634_mu4.gif" extraInfo="missing"/〉 PO43− with larger molecular weight fractions (〉50 000 and 10 000–50 000 Da) was not in general related to the quantity of humic substances of the same molecular weight in the samples. However, the proportions of 〈inlineGraphic alt="inline image" href="urn:x-wiley:00465070:FWB634:FWB_634_mu5" location="equation/FWB_634_mu5.gif" extraInfo="missing"/〉 Fe3+ and 〈inlineGraphic alt="inline image" href="urn:x-wiley:00465070:FWB634:FWB_634_mu6" location="equation/FWB_634_mu6.gif" extraInfo="missing"/〉 PO43− observed in higher molecular weight peaks were strongly correlated to the quantity of humic substances of the same molecular weight in (a) the 10 000–50 000 Da peak in the sample of low ionic strength at pH 5.5 and pH 7.0, and (b) the〉 50 000 Da peak in the sample of higher ionic strength at pH 4.0. 
3. It was concluded that humic substances promote the association of 〈inlineGraphic alt="inline image" href="urn:x-wiley:00465070:FWB634:FWB_634_mu7" location="equation/FWB_634_mu7.gif" extraInfo="missing"/〉 Fe3+ and 〈inlineGraphic alt="inline image" href="urn:x-wiley:00465070:FWB634:FWB_634_mu8" location="equation/FWB_634_mu8.gif" extraInfo="missing"/〉 PO43− with higher molecular weight fractions primarily by acting as peptizing agents for inorganic colloids containing Fe and P. Association of 〈inlineGraphic alt="inline image" href="urn:x-wiley:00465070:FWB634:FWB_634_mu9" location="equation/FWB_634_mu9.gif" extraInfo="missing"/〉 Fe3+ and 〈inlineGraphic alt="inline image" href="urn:x-wiley:00465070:FWB634:FWB_634_mu10" location="equation/FWB_634_mu10.gif" extraInfo="missing"/〉 PO43− with material of higher molecular weight via the formation of humic substance-Fe3+–PO43− complexes was identified but only at specific pH and within specific molecular weight ranges for each of the epilimnetic lake water samples studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2427.2000.00634.x
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  • 3
    Electronic Resource
    Electronic Resource
    Identification and localisation of a nucleoporin-like protein component of the plant nuclear matrix (1992)
    Springer
    Planta 187 (1992), S. 414-420 
    Details
    ISSN: 1432-2048
    Keywords: Allium (nucleoporin) ; Brassica (nucleoporin) ; Daucus (nucleoporin) ; Nucleoporin (plant) ; Nucleus (pore, matrix)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Salt-detergent extraction of purified plant nuclei yields a fraction enriched in putative structural proteins known as the “nuclear matrix”. Compared with mammalian nuclear matrices, which contain three major proteins, plant nuclear matrices are complex, containing at least 100 polypeptides. In order to characterise more fully the plant nuclear matrix we have used antibodies raised against both yeast (Saccharomyces cerevisiae) and mammalian (rat) nuclear pore proteins. We have shown that the nuclear matrix of carrot (Daucus carota L.) contains at least one nucleoporin-like protein of about 100 kDa which is immunologically related to both the yeast nuclear pore protein NSP1 and mammalian nucleoporins (p62). Antibody labelling of a variety of plant cells at the light-microscope and electron-microscope levels confirms that this antigen is located at the nuclear pores. This, to our knowledge, is the first identification of a nuclear pore protein in plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195666
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  • 4
    Electronic Resource
    Electronic Resource
    Localization of telomeres in plant interphase nuclei by in situ hybridization and 3D confocal microscopy (1991)
    Springer
    Chromosoma 100 (1991), S. 424-431 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We investigated the three-dimensional (3D) arrangement of telomeres in structurally well preserved, interphase nuclei of Pisum stativum and Vicia faba root tips using in situ hybridization of a probe to telomeric sequences. The probe was labelled with either digoxygenin or biotin and hybridized sequences were detected by immunofluorescence. Three-dimensional data sets were collected by confocal optical microscopy or using a cooled CCD camera. Twelve stacks of optical sections of P. sativum nuclei and nine of V. faba nuclei were studied in detail. Projections through the stacks of optical sections revealed that, in both species, most of the telomeres were adjacent to the nuclear envelope except for a small number next to the nucleolar periphery. In V. faba nuclei, the telomeres were clearly clustered at one pole while in P. sativum there was only a slight tendency for clustering. In V. faba, clusters were found at opposite poles in pairs of sister nuclei rather than at adjacent poles as would be expected if the arrangement at telophase were maintained into interphase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364552
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  • 5
    Electronic Resource
    Electronic Resource
    Three-dimensional organization of ribosomal DNA in interphase nuclei ofPisum sativum by in situ hybridization and optical tomography (1990)
    Springer
    Chromosoma 99 (1990), S. 143-151 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The three-dimensional (3D) organization of rDNA-containing chromatin was studied in structurally well preserved, interphase nuclei ofPisum sativum root tips by in situ hybridization using a biotinylated cDNA probe to the 18, 5.8 and 25 S rDNA sequences. The probe was detected by immunofluorescence and optical section images recorded either by video imaging or by using a confocal laser scanning microscope. Detailed 3D reconstructions were made of 12 nucleoli by projection of confocal optical sections. The probe labelled four perinucleolar heterochromatin sites, one pair 1.0–2.1 µm in diameter and the other 0.5–1.0 µm diameter. It also labelled intranucleolar structures including 300–500 nm spots emanating from the perinucleolar sites into the body of the nucleolus. The intranucleolar labelled structures emanating from the perinucleolar sites lay in discrete domains. Medium power observations of 22 fields of cells (6–30 cells per field) were made by optical sectioning using a video camera and computer deblurring. The arrangement of the perinucleolar sites was modelled in each cell and the arrangements examined for nonrandomness. The sites tended to be spaced out around the nucleolar periphery approximating a regular tetrahedral arrangement as if to minimize clustering and the large sites appeared to lie in a plane perpendicular to the root axis. Cells with multiple nucleoli did not have any preferred distribution of sites between nucleoli. These observations are discussed in terms of current models of rDNA organization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01735331
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  • 6
    Electronic Resource
    Electronic Resource
    Widely dispersed DNA within plant and animal nucleoli visualised by 3-D fluorescence microscopy (1992)
    Springer
    Chromosoma 101 (1992), S. 478-482 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ribosomal RNA (rRNA) genes in eucaryotes hold out the possibility of a temporal and spatial understanding of structural events in genetic regulation, since they are present in very large copy numbers and are extensively transcribed within a highly organised and specialised organelle-the nucleolus. However, although the existence of the genes within active nucleoli has been beyond dispute for many years, their position and arrangement has been the subject of considerable recent controversy. We have made use of the very sensitive and photometrically accurate imaging given by a cooled solid state CCD camera, coupled with computer deconvolution, to image DNA directly within nucleoli by three dimensional (3-D) optical microscopy. Our results show that DNA is more widely distributed than immunogold techniques have indicated and that in addition to brightly staining structures, corresponding to fibrillar centres, there is also a large amount of diffuse DNA, consistent with DNA location in other nucleolar components; this dispersed DNA is likely to correspond to the sites of gene transcription.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00352470
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  • 7
    Electronic Resource
    Electronic Resource
    Nucleolar organizer expression in Allium cepa L. chromosomes (1996)
    Springer
    Chromosoma 105 (1996), S. 12-19 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Roots from Allium cepa L. (cv. Francesa) bulbs in which a maximum of two nucleoli per nucleus developed were selected for this study. Five rDNA clusters were detected by fluorescent in situ hybridization on chromosomal squashes (2n = 16) with a rhodamine-labelled wheat rDNA repeat. The rDNA clusters were located on four chromosomes: the largest cluster occurred on the small arm of a single homologue of the smallest pair 8. Its homologue showed two different small rDNA clusters, one near each telomere. The two homologues of the satellited chromosomes 6 also showed different rDNA contents, which were intermediate to those found in pair 8. The same five well-differentiated hybridization signals were observed in interphase cells that were inactive in transcription because they were in dormant roots, or in proliferating ones in which the synthesis of the large rRNA precursor was prevented. After multipolarizing agent was applied in anaphase followed by inhibition of cytokinesis, multinucleate autotetraploid cells were formed, which often contained more than four nucleoli. Thus, at least two of the three nucleolar organizer regions that consistently failed to develop a nucleolus in normal mononucleate cells were capable of developing nucleoli when segregated into different nuclei in multinucleate cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004120050154
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  • 8
    Electronic Resource
    Electronic Resource
    Splicing-independent processing of plant box C/D and box H/ACA small nucleolar RNAs (1999)
    Springer
    Plant molecular biology 39 (1999), S. 1091-1100 
    Details
    ISSN: 1573-5028
    Keywords: box C/D ; box H/ACA ; intron ; processing ; small nucleolar RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Small nucleolar RNAs (snoRNAs) are involved in various aspects of ribosome biogenesis and rRNA maturation. Plants have a unique organisation of snoRNA genes where multiple, different genes are tightly clustered at a number of different loci. The maize gene clusters studied here include genes from both of the two major classes of snoRNAs (box C/D and box H/ACA) and are transcribed as a polycistronic pre-snoRNA transcript from an upstream promoter. In contrast to vertebrate and yeast intron-encoded snoRNAs, which are processed from debranched introns by exonuclease activity, the particular organisation of plant snoRNA genes suggests a different mode of expression and processing. Here we show that single and multiple plant snoRNAs can be processed from both non-intronic and intronic transcripts such that processing is splicing-independent and requires endonucleolytic activity. Processing of these different snoRNAs from the same polycistronic transcript suggests that the processing machineries needed by each class are not spatially separated in the nucleolus/nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006157022319
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  • 9
    Electronic Resource
    Electronic Resource
    Endoplasmic microtubules connect the advancing nucleus to the tip of legume root hairs, but F-actin is involved in basipetal migration (1987)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 8 (1987), S. 27-36 
    Details
    ISSN: 0886-1544
    Keywords: nuclear migration ; microtubules ; F-actin ; root hairs ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A prominent feature of tip growth in filamentous plant cells is that the nucleus often migrates in step with the tip as it extends. We have studied this long-recognized but unexplained relationship in root hairs of the legume Vicia hirsuta by a variety of microscopic techniques. Using rhodaminyl lysine phallotoxin, and antitubulin antibodies, root hairs are shown to contain axial bundles of F-actin and a complex microtubular system. To the basal side of the nucleus the microtubules are cortical and net axial but in the region between nucleus and tip the arrangement is more complicated. Electron microscopic thin sections demonstrate that internal bundles of microtubles exist in addition to the plasma membrane-associated kind. Computerized deblurring of through-focal series of antitubulin stained hairs clarifies the three-dimensional organization: bundles of endoplasmic microtubules progress from the nuclear region toward the apical dome where they can be seen to fountain out upon the cortex.The relationship between nucleus and tip can be uncoupled with antimicrotubule herbicides. Time lapse video microscopy shows that these agents cause the nucleus to migrate toward the base. This contrary migration can be inhibited by adding cytochalasin D, which fragments the F-actin bundles.It is concluded that microtubules connect the nucleus to the tip but that F-actin is involved in basipetal migration as is known to occur when symbiotic bacteria uncouple the nucleus from the tip.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970080105
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  • 10
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    Plasma protein distribution and its impact on pharmacokinetics of liposomal amphotericin B in paediatric patients with malignant diseases (2006)
    Springer
    Details
    Publication Date: 2006-12-19
    Print ISSN: 0031-6970
    Electronic ISSN: 1432-1041
    Topics: Chemistry and Pharmacology , Medicine
    Published by Springer
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