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  • 1
    Electronic Resource
    Electronic Resource
    Nonspecific integration of the HTLV provirus genome into adult T-cell leukaemia cells (1984)
    [s.l.] : Nature Publishing Group
    Nature 309 (1984), S. 640-642 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] As previously reported, the leukaemic cells of most ATL patients contain one or two copies of HTLV provirus4'11, but the size of the coRI-digested DNA fragment containing the provirus and cellular flanking sequences differs between individuals, suggesting that the integration sites of the HTLV ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/309640a0
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  • 2
    Electronic Resource
    Electronic Resource
    A matrix metalloproteinase expressed on the surface of invasive tumour cells (1994)
    [s.l.] : Nature Publishing Group
    Nature 370 (1994), S. 61-65 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We screened cDNAs with homology to conserved regions in matrix metalloproteinase (MMP) genes (Fig. 1 legend) and iso-lated a unique 3.4-kilobase (kb) cDNA fragment (MMP-X1) from a human placenta cDNA library. This cDNA encodes a unique protein of 582 amino acids (Mr 66K) which can be closely ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/370061a0
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  • 3
    Electronic Resource
    Electronic Resource
    Structure of the region of the replication origin of the Bacillus subtilis chromosome (1979)
    [s.l.] : Nature Publishing Group
    Nature 281 (1979), S. 699-701 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A thymine-requiring mutant of B. subtilis, CRK2000 (trp, leu, thy)7, and its ts-dna mutant derivative, CRK2001 (trp, leu, thy, dints)& which is defective in the initiation of DNA replication at 47 C, were used to label the DNA at the origin of replication. Initiation of the replication cycle ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/281699a0
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of novobiocin on initiation of DNA replication in Bacillus subtilis (1979)
    [s.l.] : Nature Publishing Group
    Nature 281 (1979), S. 702-704 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We used a spore germination of a thymine-requiring mutant of B. subtilis, CRK2000 (leu, trp, thy)9, to synchronise the initiation of replication. The mutant spores were germinated in a synthetic medium, GM11 (réf. 10), in the absence of thymine for 150 min; 3H-thymidine (dThd) was then added ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/281702a0
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  • 5
    Electronic Resource
    Electronic Resource
    Differentiation-dependent expression of gelatinase B/matrix metalloproteinase-9 in trophoblast cells (1999)
    Springer
    Cell & tissue research 295 (1999), S. 287-296 
    Details
    ISSN: 1432-0878
    Keywords: Key words Trophoblast giant cell ; Placenta ; Gelatinase B/MMP-9/92-kDa gelatinase ; Ectoplacental cone outgrowths ; Invasion ; trophoblast ; Rat (Sprague Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The purpose of this study was to evaluate the Rcho-1 trophoblast culture system as a model for studying trophoblast invasion and to examine stage-specific expression of enzyme(s) potentially participating in rat trophoblast giant cell invasive behavior. The invasive behavior of the differentiating Rcho-1 trophoblast cells was demonstrated using Matrigel invasion chambers. Gelatin zymography and Western blot analysis of conditioned medium from differentiating Rcho-1 trophoblast cell cultures and rat ectoplacental cone outgrowths revealed a differentiation-dependent increase in gelatinase B/matrix metalloproteinase (MMP-9). Nothern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analyses of Rcho-1 trophoblast or ectoplacental cone cells also showed increasing expression of MMP-9 accompanying cell differentiation. Rcho-1 trophoblast cells stably transfected with MMP-9 promoter/luciferase reporter constructs exhibited a differentiation-dependent increase in MMP-9 promoter activation. In conclusion, trophoblast giant cell differentiation is characterized by transcriptional activation of the MMP-9 gene and appearance of the invasive phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410051235
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  • 6
    Electronic Resource
    Electronic Resource
    Initiation of DNA replication in Bacillus subtilis (1980)
    Springer
    Molecular genetics and genomics 179 (1980), S. 265-272 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of an intercalating dye, ethidium bromide (EtBr), on the initiation of chromosome replication in Bacillus subtilis were studied. Spores of a thymine requiring mutant acquired the ability to initiate one round of replication in the absence of RNA and protein synthesis (initiation potential) during germination in a thymine starved medium. When EtBr was added after the initiation potential was fully established, initiation of replication was completely inhibited. This inhibition was reversible, and initiation was resumed when the drug was removed. The recovery of initiation occurred in the absence of protein synthesis but did require RNA synthesis and an active dna gene product. During germination both a DNA-protein complex and a DNA-membrane complex were formed at the replication origin in parallel with the establishment of initiation potential. EtBr destroyed both of these complexes at the concentration which inhibited initiation. The first round of replication of a plasmid DNA, pSL103, during spore germination was also prevented by EtBr. However a higher concentration was required to inhibit plasmid replication. It was found that the plasmid formed two complexes identical to the S- and M-complex of the chromosome origin. Compared to the chromosome complexes the plasmid complexes were less sensitive to EtBr. The loss of sensitivity was equivalent to that for the initiation of the plasmid compared to the chromosome. These results indicate that the target of EtBr is the DNA in the S- and M-complexes whose conformation is essential for the initiation of chromosome and plasmid replication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425453
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  • 7
    Electronic Resource
    Electronic Resource
    Initiation of DNA replication in Bacillus subtilis (1981)
    Springer
    Molecular genetics and genomics 181 (1981), S. 332-337 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When spores of a thymine-requiring mutant of Bacillus subtilis were germinated in a medium lacking thymine, an initiation potential (an ability to initiate and complete one round of replication in the presence of thymine and in the absence of protein and RNA synthesis) was formed for both chromosomal and plasmid replication. The effect of two inhibitors of DNA gyrase, novobiocin (Nov) and nalidixic acid (Nal), on the initiation potential formed during germination for chromosomal and plasmid replication was examined. Nov and Nal inhibited formation of the initiation potential completely if the drug was added at the onset of germination. In contrast, initiation of chromosomal and plasmid replication occurred in the presence of DNA gyrase inhibitors when the drug was added after the initiation potential had been fully formed. However, chromosomal replication initiated in the presence of the inhibitors ceased after a fragment of approximately 15 MD (15×106 daltons) had been replicated, and plasmid replication was limited to one round of replication in approximately half of the plasmid molecules present in the spores. Furthermore the initiation potential for both chromosomal and plasmid replication though established was destroyed gradually but steadily by prolonged incubation with Nov in the absence of thymine. In addition, relaxation of the superhelical structure of plasmid DNA during incubation with Nov was observed in vivo. This relaxation was blocked by ethidium bromide, which dissociated the S-complex. On the other hand, incubation with Nal did not reduce the initiation potential nor did it change the superhelicity of the plasmid DNA in vivo. This is consistent with the known effect of gyrase inhibitors on the enzymatic activity of DNA gyrase. These results clearly demonstrate that both the action of DNA gyrase and the superhelical structure of the DNA are essential for the initiation of chromosomal and plasmid replication. The specific chromosome organization essential for initiation and elongation and the role of DNA gyrase are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425607
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  • 8
    Electronic Resource
    Electronic Resource
    Structure and function of the region of the replication origin of the Bacillus subtilis chromosome (1981)
    Springer
    Molecular genetics and genomics 183 (1981), S. 220-226 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A BamHI restriction endonuclease fragment, B7, which is replicated first among all other fragments derived from the Bacillus subtilis chromosome, was cloned in Escherichia coli using as vector a hybrid plasmid pMS102 that can replicate both in E. coli and B. subtilis. Digestion of pMS102 with BamHI produced two fragments and the smaller one was replaced by the B7 fragment. The cloned plasmid pMS102′-B7 exhibited some peculiar properties that were not observed with plasmids containing other fragments from the B. subtilis chromosome. (1) E. coli cells harboring this plasmid stuck to each other and to glass. This property was more apparent when cells were grown in poor media. (2) E. coli cells tended to lose the plasmid spontaneously when they were grown without the selective pressure favorable to the plasmid. (3) The frequency of transformation of B. subtilis by pMS102′-B7 was less than 1/1,000 of that by the vector plasmid pMS102′. The number of copies of pMS102′-B7 present in the transformants was also markedly reduced, although the pUB110 origin of replication on the vector was intact and should be functional in B. subtilis. This inhibitory effect of the B7 fragment on plasmid replication was confirmed more directly by developing a semi in vitro replication system using protoplasts. Both in E. coli and B. subtilis the B7 fragment affected replication of its own molecule but not that of the coexisting plasmid with an identical replication system. The implication of the function of the B7 fragment in the initiation of the B. subtilis chromosome will be discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270621
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  • 9
    Electronic Resource
    Electronic Resource
    Structure and function of the region of the replication origin of the Bacillus subtilis chromosome (1981)
    Springer
    Molecular genetics and genomics 183 (1981), S. 227-233 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A BamHI restriction endonuclease fragment B7, which contains the replication origin of the Bacillus subtilis chromosome, showed inhibitory effects on cell growth and plasmid replication in Escherichia coli and Bacillus subtilis, when B7 was inserted into a composite plasmid pMS102′ and introduced into these cells. In order to localize these properties in more limited regions within the B7 fragment, we developed a new and widely applicable method for deletion of DNA segments of various lengths from one or other end of a given region of the plasmid molecule. Using a set of deletions in the B7 fragment of pMS102′-B7, we determined the loci responsible for the inhibitory effects of B7 as described below. (1) Stickiness appearing in E. coli cells was caused by a segment residing in a region of approximately 2.2 kilobase pairs (kbp) overlapping the E19 and E22 fragments. (2) Instability of the plasmid in E. coli was due to a segment localized in the 440 bp region of the E19-side terminal portion of the 2.2 kbp region. (3) The same 440 bp were also responsible for inhibition of the replication of the plasmid in B. subtilis. Hybridization of the cloned DNA fragments containing the 2.2 kbp region with the whole B. subtilis chromosome revealed that several regions of the chromosome are homologous to this characteristic sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270622
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  • 10
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    Mint3 potentiates TLR3/4- and RIG-I–induced IFN-β expression and antiviral immune responses (2016)
    National Academy of Sciences
    Details
    Publication Date: 2016-10-03
    Description: Type I IFNs (IFN-α/β) play crucial roles in the elimination of invading viruses. Multiple immune cells including macrophages recognize viral infection through a variety of pattern recognition receptors, such as Toll-like receptors (TLRs) and retinoic acid-inducible gene-I (RIG-I)–like receptors, and initiate type I IFN secretion and subsequent antiviral immune responses. However, the mechanisms by which host immune cells can produce adequate amounts of type I IFNs and then eliminate viruses effectively remain to be further elucidated. In the present study, we show that munc18-1–interacting protein 3 (Mint3) expression can be markedly induced during viral infection in macrophages. Mint3 enhances TLR3/4- and RIG-I–induced IRF3 activation and IFN-β production by promoting K63-linked polyubiquitination of TNF receptor-associated factor 3 (TRAF3). Consistently, Mint3 deficiency greatly attenuated antiviral immune responses and increased viral replication. Therefore, we have identified Mint3 as a physiological positive regulator of TLR3/4 and RIG-I–induced IFN-β production and have outlined a feedback mechanism for the control of antiviral immune responses.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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