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  • 1
    Electronic Resource
    Electronic Resource
    Increased sensitivity and discrimination in screening through an immobilized-resin microbiological assay method (1987)
    Springer
    Journal of industrial microbiology and biotechnology 2 (1987), S. 243-249 
    Details
    ISSN: 1476-5535
    Keywords: Bioassay ; Polymeric resin ; Bioscreening ; Antibiotic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Problems with present bioactive microbial product screening techniques include low sensitivity and insufficient discrimination capabilities. These problems are addressed by our new immobilized-resin microbiological assay. This technique concentrates bioactive samples on macroporous polymeric resins that are immobilized in hydrogel beads. These beads are then subjected to elution in the wells of an agar diffusion microbiological assay medium. With a strong base anion exchanger, the sensitivity to ampicillin of the β-lactam-supersensitiveEscherichia coli mutant ESS-22-31 was increased 10-fold. Similar increases in sensitivity were obtained in the detection of streptomycin using a weak acid cation exchanger withBacillus subtilis and for cycloheximide by a neutral resin andSaccharomyces cerevisiae NRRL-Y-139. A judicious choice of resin type and eluent permitted a selective sensitivity increase based on the charge or hydrophobic nature of the desired product. This selectivity imparts a discrimination capability to the technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569546
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  • 2
    Electronic Resource
    Electronic Resource
    Fermentation monitoring by polymeric-resin-based microbiol assay (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 25 (1983), S. 771-779 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A polymeric-resin-based microbiological assay is shown to yield results consistent with classical inhibition zone theory. This novel assay is applicable to cycloheximide, an antifungal antibiotic, at concentrations below 1.0 μg/mL and up to 800 μg/mL. Addition of ethanol to the cut-out wells of the assay plate helps to release the antibiotic and drastically increases the inhibition zone size. The antibiotic concentrations of both two component and multicomponent systems can be determined using this method. The use of polymeric-resin beads to monitor antibiotic concentrations is shown to be an effective technique for fermentation monitoring.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260250313
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