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  • 1
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Recent improvements both in the staining quality and computer-aided quantitation of silver-stained nucleolar organizer region (AgNOR)-associated proteins offer the possibility to reliably investigate these proteins on routinely processed archival material. This article deals with the historical background, the recent introduction of a standardized quantitation, the clinical relevance, and future perspective for AgNOR evaluation. It is specifically emphasized that AgNOR analysis after both standardized staining and computer-aided quantitation (as recommended by the Committee on AgNOR Quantitation of the European Society of Pathology) is now regarded as the gold standard whenever routinely formalin-fixed and paraffin-embedded material is investigated.
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  • 2
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The co-localization of various antigens in rat chromaffin granules was investigated by the immunogold staining procedure. In ultrathin serial sections staining of chromaffin granules was obtained with antisera against chromogranin A, chromogranin B, secretogranin II and neuropeptide Y. These results indicated that these antigens are costored within chromaffin granules. To further corroborate this point a double immunogold staining procedure was used. This method unequivocally established that chromogranin A, chromogranin B, secretogranin II and neuropeptide Y are co-localized in the same chromaffin granules. These results are relevant for studies demonstrating changes in the level of these peptides in adrenal medulla. The co-localization makes it likely that such changes lead to a different relative composition of the secretory quanta of chromaffin granules.
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  • 3
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Analysis of human and bovine serum by immunoblotting revealed the presence of the proprotein chromogranin A. By the same method chromogranin A was also found in rat, bovine and human kidney. However this organ did not contain any chromogranin A mRNA arguing against a synthesis within this organ. By immun-electron microscopy chromogranin A immunoreactivity was found in proximal tubule cells of rat kidney. Positive immunostaining was present in small vesicles within and in close proximity to the brush border and closer to the nucleus in typical lysosomal structures. These results make it likely that chromogranin A from serum reaches kidney tubule cells by glomerular filtration and is taken up into the endocytotic lysosomal pathway.
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  • 4
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A theory in the spirit of the Hartree-Fock (HF) model is formulated which takes into account general types of correlation effects. This theory, dubbed as Multi-Configuration Hartree-Fock (MCHF) model, makes use of a multideterminantal trial wave function. In the present work the intrinsic ground state wave functions obtained in this theory have been studied. Doubly evenN=Z andN=Z + 2 nuclei in thesd-shell have been treated and the results have been compared against the HF-predictions. While the HF-approximation is found to be quite good forN=Z nuclei, correlations are found to play a strikingly significant role in theN=Z +2 nuclei.
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  • 5
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The intrinsic ground state wave functions obtained for doubly evens d-shell nuclei using the Multi-Configuration-Hartree-Fock (MCHF) model have been analysed in the first part of this paper series. Here we study the effect of the generalized correlations contained in the MCHF-solutions on the ground state rotational bands, by projecting out states of good angular momentum. The results show that those spectra are quite sensitive to correlations. For theN=Z+2 nuclei, where correlations have been found to be significant, major improvements compared to corresponding Hartree-Fock spectra are obtained. Different effective forces have been used to illustrate that our conclusions are force independent. In most cases, the calculated excitation energies as well as theB(E2) transition values are in rather good agreement at least with the gross properties of the experimental data.
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  • 6
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The one body matrix elements of the effective quadrupole operator in the 2p 3/2, 1f 5/2 2p 1/2 configurations have been calculated taking into account the renormalization effects due to excitations from the closed 1f 7/2 shell. First and second order perturbations of the residual interaction as well as an infinite series involving one particle-one hole core excitations are considered. The results are used to calculate quadrupole moments of odd mass nuclei together withBE 2 values between low-lying states. Agreements with experimental results, whereever known, are seen to be generally greatly improved.
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  • 7
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Competition of strong and decoupling is studied systematically for odd-odd mass nuclei as a function of the position of the Fermi surfacesε F (π) andε F (υ) and the deformationsβ andγ. As an example we use80Br. For a prolate deformation the odd proton likes to decouple while the odd neutron couples strongly. This favours a ground state angular momentum of 5 to 6. The strong coupling is described by the admixture of ad 5/2 nucleon. Only for the neutrons the admixture is so appreciably that it affects the spectrum and improves the agreement between theory and experiment for80Br.
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  • 8
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract An attempt is made to take into account single particle degrees of freedom in angular momentum projected HFB-calculations via the explicit inclusion of two quasiparticle states. For this purpose a formalism is used which, though being rather general, nevertheless is rather suitable for numerical applications even in realistic model spaces. As a first test the method is applied in thei 13/2-shell, where exact shell model solutions are available. Though using only a rather truncated quasiparticle space we obtain almost perfect agreement with these exact solutions.
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  • 9
    ISSN: 1434-601X
    Keywords: 21.60.Cs ; 21.60.Jz
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract An expression is derived for the translationally invariant (transition-) charge density in the center of mass rest frame of the nucleus in between arbitrary wave functions. An explicit formulation is given for the charge density of spherically symmetric Hartree-Fock type ground states. The method is applied to the nuclei4He,16O,40Ca and90Zr using Woods-Saxon single nucleon orbits for the construction of the ground state wave functions. For the two lighter nuclei in addition realistic Hartree-Fock wave functions are investigated. The results are compared to those obtained with various approximate treatments of the center of mass motion. It turns out that at least in the lighter nuclei the usual center of mass correction, which is based on the assumption of pure non-spurious oscillator configurations and commonly used in the analysis of, e.g., electron scattering data, does not produce reliable (or even predictable) results. Thus here indeed the full translationally invariant treatment seems to be required. For the heavy nucleus90Zr on the other hand, as expected, the center of mass effects become negligibly small.
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  • 10
    ISSN: 1434-601X
    Keywords: 21.60.Cs ; 21.60.Jz
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Microscopic nuclear structure wave functions obtained within the EXCITED FED VAMPIR approach are used to calculate the charge densities for the ground states as well as the transition charge densities for the transitions from the ground to the first and second 2+ states in some doubly even Ge isotopes. The results are compared with the data extracted from elastic and inelastic electron scattering experiments. Except for the heavier isotopes, where the calculations fail to yield enough collectivity, rather nice agreement is obtained. This result strongly supports the theoretically predicted shape coexisting structures in the low spin states of the nuclei in this mass region.
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