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  • 1
    Publication Date: 1979-10-01
    Print ISSN: 0014-4754
    Topics: Biology , Medicine
    Published by Springer
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  • 2
    Publication Date: 2008-04-22
    Print ISSN: 0018-8158
    Electronic ISSN: 1573-5117
    Topics: Biology
    Published by Springer
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 46 (1997), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Strains of Erwinia herbicola effective in the biocontrol of fire blight of hawthorn were used to investigate the possibility that the antagonistic activity is coded by plasmid-born genes. Agarose gel electrophoresis of isolated plasmids from four antagonistic Erw. herbicola strains showed a band of a supercoiled 12 kb plasmid in each strain, with a second band greater than 16.2 kb consistently seen in two strains. Erw. herbicola strains showed resistance to penicillin-G, which could be conferred on penicillin-G sensitive Escherichia coli TG1 by transformation with a pure Erw. herbicola plasmid preparation. Transformed strains of Esc. coli appeared to contain the Erw. herbicola 12 kb plasmid, but not the 〉 16.2 kb plasmid. In an agar plate assay, Esc. coli transformants produced an inhibition zone against Erw. amylovora similar to those produced by the original Erw. herbicola strains. In two biocontrol assays, the transformed Esc. coli strains had a suppressive effect on disease development on infected pear fruit slices and hawthorn blossoms.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Post-harvest rotting of Dutch white cabbage was reduced by post-harvest treatment with Pseudomonas fluorescens strains CL42, CL66, CL82, Serratia plymuthica strain CL43 or Serratia liquefaciens CL80, in three storage trials carried out in an experimental cold store at Manchester University, Manchester, UK and in a commercial cold store at L.W. van Geest Farms Ltd, Spalding, UK. The amount of surface area covered by fungal growth was assessed at 6-week intervals during storage and the trimming losses were determined after 8 to 10 months. Only strains CL80 and CL82 were found to reduce fungal spoilage significantly in all three trials, and control by strain CL82 was similar to that achieved by post-harvest treatment with fungicides. In the commercial cold store, CL42 showed better results than any of the other bacterial strains.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Bacterial antagonists against Botrytis cinerea were isolated from different Brassica spp. and identified. All isolates showing in vitro antagonism at 4°C were shown to be either fluorescent pseudomonads or Serratia spp. In vitro antagonism against B. cinerea and Alternaria brassicicola was found to depend on the temperature and concentration of nutrients in the medium.Bacterial strains which showed in vitro antagonism were tested for in vivo antagonism at 4°C against B. cinerea and A. brassicicola using a leaf disc bioassay. Pseudomonas fluorescens isolates CL42, CL66, CL82 and Serratia plymuthica strain CL43 showed inhibition of Botrytis growth on leaf discs; P. fluorescens isolate CL74 and all Serratia liquefaciens isolates exhibited intermediate control. All other fluorescent pseudomonad isolates showed poor control or caused rotting of the cabbage tissue.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 39 (1990), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Isolates of Erwinia herbicola, obtained from flowers and leaves of hawthorn (Crataegus monogyna), were screened as potential control agents of fire blight disease (caused by Erwinia amylovora) using an immature pear fruit assay. Selected isolates were subsequently tested for disease control by infection of hawthorn blossom in the laboratory, and by shoot infection of hawthorn plants grown under controlled (glasshouse) and fluctuating (polythene tunnel) environmental conditions.Although the immature pear fruit assay provided a general screen for the selection of antagonists for the control of both blossom and shoot blight, it had two major limitations when quantitatively applied. Firstly there were inconsistencies in the relative effects of different isolates on the pear-slice surface, with some isolates being more suppressive than the standard antagonist Eh252 in the first screening and less in the second. Secondly the assay was not able to predict accurately the level of control in the intact plant-as no correlation occurred between the level of control in the pear fruit assay and the percentage control of either blossom blight or shoot blight.Two isolates of E. herbicola, WL9 and WL40, reduced both blossom- and shoot-blight. WL9 provided over 80% control of blossom blight, equivalent to that provided by chemical agents, and also gave total control of shoot blight when applied at a WL9: pathogen ratio of 10:1.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 90 (1976), S. 333-341 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ligule ofSelaginella kraussiana shows active incorporation of tritiated glucose in the central region; particularly into the Golgi system, but also into endoplasmic reticulum, mitochondria and cell periphery. Two hours chase in unlabelled glucose reveals a small amount of cell wall formation but most of the label remains in the Golgi bodies. The results suggest that the Golgi system in the mature ligule has the capacity to synthesise some complex carbohydrate, but is relatively inactive in its secretion. This is discussed in relation to the ontogeny and phyllogeny of the ligule.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 75 (1972), S. 323-334 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The replication of nuclear and cytoplasmic DNA during the final stages of oogenesis inPteridium aquilinum was investigated using light and electron microscope autoradiography. No nuclear S phase occurs during the development of the egg cell itself, though it was present in the preceding central cell. Cytoplasmic replication was continuous throughout. The large amount of cytoplasmic DNA in the mature egg cell was concluded to be derived mainly as a result of cytoplasmic replication and not large scale efflux from the nucleus. It is proposed that some of the organelles degenerate during the final development of the egg cell, leading to a high proportion of DNA in the groundplasm. Some of the mitochondria and plastids persist, the mitochondria undergoing considerable change. There is thus some continuity in transmission of organelles from gametophyte to sporophyte generation.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 35 (1979), S. 1332-1334 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary X-ray microanalysis of fixed, sectioned chromosomes of the dinoflagellatesGlenodinium foliaceum, Prorocentrum micans andAmphidinium carterae has revealed high levels of iron, nickel, copper and zinc. We report high levels of these transition metals in association with chromosomes in intact eukaryote cells.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 414 (1999), S. 75-81 
    ISSN: 1573-5117
    Keywords: Anabaena ; SEM XRMA ; population development ; elemental associations ; structural model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in the elemental composition of the blue-green alga Anabaena at Rostherne Mere (Cheshire, U.K.) were studied over an extended bloom period in 1996. Elemental analysis was carried out in relation to intracellular concentrations, intracellular elemental correlations, time series correlations and total quantities of elements in lake water. Most of the intracellular elements showed considerable variation both between and within samples. Correlations between these elements also varied considerably, though certain pairs – K/Cl, K/Mg, P/K and P/Mg were significantly correlated in at least 50% of the samples. Analysis of the time series demonstrated clear correlations between lake water P and intracellular P and Mg. The growth of Anabaena coincided with a considerable increase in the overall amount of chemicals contained in its population. The total amounts of chemicals in the lake water, however, always substantially exceeded those present in the population of Anabaena cells. Elemental relationships within cells are considered in relation to an elemental correlation pattern, consisting of three main groupings – Mg-K-P, Na-S and Ca-Cl. The structural model of intracellular elemental associations presented here may be helpful in the investigation of algal response to changes in environmental parameters.The incorporation of this model into a dynamic model simulating phytoplankton–environmental interactions is discussed.
    Type of Medium: Electronic Resource
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