Publication Date:
2012-05-05
Description:
Medical applications of nanotechnology typically focus on drug delivery and biosensors. Here, we combine nanotechnology and bioengineering to demonstrate that nanoparticles can be used to remotely regulate protein production in vivo. We decorated a modified temperature-sensitive channel, TRPV1, with antibody-coated iron oxide nanoparticles that are heated in a low-frequency magnetic field. When local temperature rises, TRPV1 gates calcium to stimulate synthesis and release of bioengineered insulin driven by a Ca(2+)-sensitive promoter. Studying tumor xenografts expressing the bioengineered insulin gene, we show that exposure to radio waves stimulates insulin release from the tumors and lowers blood glucose in mice. We further show that cells can be engineered to synthesize genetically encoded ferritin nanoparticles and inducibly release insulin. These approaches provide a platform for using nanotechnology to activate cells.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646550/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646550/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stanley, Sarah A -- Gagner, Jennifer E -- Damanpour, Shadi -- Yoshida, Mitsukuni -- Dordick, Jonathan S -- Friedman, Jeffrey M -- R01 GM095654/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2012 May 4;336(6081):604-8. doi: 10.1126/science.1216753.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22556257" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Bioengineering
;
Blood Glucose/*analysis
;
Calcium/*metabolism
;
Embryonic Stem Cells/metabolism
;
Epitopes
;
*Ferric Compounds
;
Ferritins/administration & dosage/genetics/metabolism
;
HEK293 Cells
;
Hot Temperature
;
Humans
;
Insulin/blood/genetics/*metabolism
;
Male
;
*Metal Nanoparticles
;
Mice
;
Mice, Nude
;
Neoplasm Transplantation
;
Neoplasms, Experimental/blood/pathology
;
PC12 Cells
;
*Radio Waves
;
Rats
;
Recombinant Fusion Proteins/administration & dosage
;
TRPV Cation Channels/genetics/immunology/*metabolism
;
Transfection
;
Transplantation, Heterologous
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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