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  • 1
    ISSN: 1617-4623
    Keywords: MAP kinases ; SLT2 gene ; Cell integrity ; Yeast autolysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Deletion of the SLT2 gene of Saccharomyces cerevisiae, which codes for a homologue of MAP (mitogen-activated) protein kinases, causes an autolytic lethal phenotype in cells grown at 37° C. The gene encodes domains characteristic of protein kinases, which include a lysine (at position 54) that lies 19 residues from a glycine-rich cluster, considered to be the putative ATP binding site. The ability of three mutant alleles of SLT2 generated by site-directed mutagenesis, namely E54 (glutamic acid), R54 (arginine) and F54 (phenylalanine), to complement slt2 mutants was tested. All three failed to complement the autolytic phenotype and were unable to restore growth and viability of cells. A strain obtained by transplacement of slt2-F54 also behaved as a thermosensitive autolytic mutant. By immunoprecipitation with polyclonal antibodies raised against Slt2 protein expressed in Escherichia coli, it was possible to confirm that alteration of the lysine-54 residue did not affect the stability of the protein, thus allowing us to conclude that activity of the Slt2 protein kinase is critically required for growth and morphogenesis of S. cerevisiae at 37° C. A significant fraction of the mutant cell population lysed at 24° C and the cells displayed a characteristic alteration of the surface consisting of a typical depression in an area of the cell wall. At 37° C, the cell surface was clearly disorganized.
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  • 2
    ISSN: 0749-503X
    Keywords: SEC14 ; Candida albicans ; protein secretion ; pathogenic fungi ; PI-TP ; Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The yeast SEC14 gene product is required for the transport of proteins from the Golgi complex. We have cloned the homologous Candida albicans SEC14 gene (CaSEC14) by functional complementation of a Saccharomyces cerevisiae thermosensitive mutant, sec14. Some putative TATA boxes have been identified in CaSEC14 and, contrary to S. cerevisiae SEC14, no introns were found in the Candida homologue. Sequence analysis revealed that CaSec14p is a 301 amino acid protein, 67% identical to S. cerevisiae and Kluyveromyces lactis Sec14p, and 61% identical to the 300 amino-terminal residues of Yarrowia lipolytica Sec14p. Hydrophatic profile analysis of CaSec14p suggests a soluble protein without transmembrane domains, as has been described for the S. cerevisiae counterpart. While it was easy to disrupt one allele of SEC14 in C. albicans, repeated attempts to disrupt the second allele were unsuccessful, thus suggesting that the gene could be essential for vegetative growth in C. albicans. The sequence has been deposited in the EMBL data library under Accession Number X81937.
    Additional Material: 4 Ill.
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  • 3
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; chromosome VII ; ribonuclease PH ; HGH1 ; YGR187c ; YGR189c ; YGR194c ; YGR195w ; YGR196c ; YGR198w ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have deleted six different ORFs of unknown function located on the right arm of Saccharomyces cerevisiae chromosome VII; namely, YGR187c/HGH1, YGR189c, YGR194c, YGR195w, YGR196c and YGR198w. No basic phenotypes could be attributed to the strains deleted in any of genes YGR187c/HGH1, YGR189c, YGR194c and YGR196c. These deletants did not show mating, sporulation or growth defects under any of the conditions tested. However, spores bearing deletions in either the YGR195w or YGR198w genes were unable to develop into macroscopical colonies. The YGR195w gene product shows significant homology with bacterial ribonuclease PH, an enzyme hitherto undescribed in yeasts, and its deletion causes a loss of viability after one to three rounds of cell division. Overexpression of this gene, using a tetracycline-regulatable promoter system, did not cause any effect on the cells. Contrary to what has been reported for prokaryotic homologs, this enzyme could play an essential role in yeast cell biology. The product encoded by the other essential ORF, YGR198w, shows no significant homology with any protein of known function in the databases. Spores bearing the deletion usually germinate and give rise to microcolonies of 50-100 non-viable cells. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 2 Ill.
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  • 4
    ISSN: 1572-9532
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A new technique is introduced in order to solve the following question:When is a complete spacelike hypersurface of constant mean curvature in a generalized Robertson-Walker spacetime totally umbilical and a slice? (Generalized Robertson-Walker spacetimes extend classical Robertson-Walker ones to include the cases in which the fiber has not constant sectional curvature.) First, we determine when this hypersurface must be compact. Then, all these compact hypersurfaces in (necessarily spatially closed) spacetimes are shown to be totally umbilical and, except in very exceptional cases, slices. This leads to proof of a new Bernstein-type result. The power of the introduced tools is also shown by reproving and extending several known results.
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  • 5
    ISSN: 1572-9532
    Keywords: GENERALIZED ROBERTSON-WALKER SPACETIME ; GEODESIC CONNECTEDNESS ; GEODESIC COMPLETENESS ; STABILITY OF COMPLETENESS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The geometry and, especially, the geodesics of a class of spacetimes generalizing Robertson-Walker ones (without any assumption on the fiber) is studied, under a global point of view. Our study covers geodesic connectedness, geodesic completeness and stability of completeness.
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  • 6
    ISSN: 1572-9532
    Keywords: LORENTZ METRICS ; GEODESIC CONNECTEDNESS ; REISSNER-NORDSTRÖM SPACETIME ; CAUSALITY
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A detailed study of the existence, causal character and multiplicity of geodesics joining two points is carried out for a wide family of non-static Lorentz manifolds (including intermediate Reissner-Nordström, inner Schwarzschild and Generalized Robertson-Walker spacetimes). Results relating causality and connectedness by timelike or lightlike geodesics are obtained, in the spirit of the well-known Avez-Seifert result. The existence of closed spacelike geodesics is also characterized.
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  • 7
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    Journal of Mathematical Physics 34 (1993), S. 3768-3774 
    ISSN: 1089-7658
    Source: AIP Digital Archive
    Topics: Mathematics , Physics
    Notes: The process to obtain a geodesic as a limit from a sequence of geodesics in a Lorentzian manifold is analyzed in detail. It is found that the limit of incomplete geodesics in a compact manifold is not incomplete in general. A new class of incomplete compact Lorentzian manifolds, one of them showing this anomalous behavior, is constructed. Thus, a counterexample to the proof, given by U. Yurtsever [J. Math. Phys. 33, 1295 (1992)] to the result originally proven by Y. Carrière [Invent. Math. 95, 615 (1989)] (in a quite different way) is given. That proof should imply that an incomplete compact Lorentzian manifold must be null incomplete. It is not known if this assertion is true, and, hence, remains as an open question.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Geometriae dedicata 53 (1994), S. 103-117 
    ISSN: 1572-9168
    Keywords: 53C22 ; 53C50
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Semi-Riemannian manifolds with a suitable set of conformal symmetries are shown to be complete. Locally warped products are studied and warped-completeness is introduced. In the case of definite and complete basis, several assumptions on the growth of the warping function yield some of the three kinds of completeness. The case of 1-dimensional basis (including a known family of relativistic space-times) is specially studied. Null warped-completeness is related to the completeness of a certain conformal metric on the basis. Several examples and counter-examples explaining the main results are also given.
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  • 9
    ISSN: 0009-286X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
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  • 10
    ISSN: 1434-1948
    Keywords: Pyrazolate complexes ; Palladium ; Platinum ; N ligands ; Bridging ligands ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Trinuclear complexes containing pyrazolate bridging ligands of the type [NBu4]2[{(C6F5)2 M(μ-pz)(μ-X)}2M′] (M, M′= Pd or Pt; X = Cl, OH, or pz) (Hpz = pyrazole) have been prepared using [NBu4][M(C6F5)2(acac)] (acac = acetylacetonate) or [NBu4]2[M2(C6F5)4(μ-OH)2] and [M′Cl2(Hpz)2] or [M′(Hpz)4]2+ as starting materials. Asymmetric homo- and heterobimetallic complexes of the types [NBu4][R2M(μ-pz)2Pd(η3-allyl)] and [R2M(μ-pz)2M′L2] (allyl = C3H5 or C4H7; L2 = 2 PEt3 or bipy; R = C6F5 or C6Cl5; M, M′ = Pd or Pt) have been obtained starting from [NBu4][MR2(pzHpz)] and [Pd(η3-allyl)(acac)] or [M′L2Cl2], respectively. The identity of the new complexes has been established by NMR (1H, 19F and 31P) spectroscopy. The crystal structure of [{(C6F5)2Pd(μ-pz)(μ-Cl)}2Pd]2- has an inversion centre, with a bent appearance of the Pd3(μ-pz)2(μ-Cl)2 moiety. The crystal structure of [(C6F5)2Pd(μ-pz)2Pd(η3-C4H7)] has also been determined by single-crystal X-ray diffraction, where a boat conformation of the central “Pd2N4” six-membered rings is observed.
    Additional Material: 2 Ill.
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