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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 194 (1962), S. 294-295 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Examination in cold-blooded animals would offer the advantage of a considerably wider physiological range of temperatures within which the reaction could be studied. Also, the possibility of obtaining this reaction in an in vitro system may be greater with the lens of a cold-blooded animal. Fig. ...
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 206 (1965), S. 1267-1269 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] For such investigations tissue of poikilothermic origin would appear to be superior in certain respects to that of homoiotherms. For example, with the former it might be possible to establish in vitro cultures more conveniently. And if these cultures proved successful it should be an easy matter to ...
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  • 3
    ISSN: 0886-1544
    Keywords: NBD-phallacidin ; actin ; ocular tissues ; wound repair ; stress fibers ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The fluorescent derivative of the actin-binding toxin phallacidin, 7-nitrobenz-2-oxa-1,3 diazole phallacidin, has been used to cytologically demonstrate the presence of actin in lens epithelium, corneal endothelium, and retinal pigment epithelium. In these noninjured tissues, no stress fibers are observed and fluorescence is confined mainly to an area at or near the cell membrane, although some diffuse cytoplasmic staining can also be seen. However, following injury to either the lens epithelium or corneal endothelium of rats and frogs, stress fibers are detected, but only in those cells that migrate into the wound area. Cells on the periphery of each tissue do not partake in would repair and thus maintain their normal appearance. After the tissue has regenerated, stress fibers disappear, and those cells involved in the injury response return to their normal morphology.When rabbit corneal endothelium is placed in tissue culture, stress fibers are observed as the cells migrate away from the initial explant. Upon reaching confluency, these cells spread out and each is surrounded by thick actin-containing bands. Furthermore, they exhibit some stress cables within their cytoplasm. This is in contrast to their appearance in vivo where stress fibers are absent and fluorescence is limited to a region near the cell membrane.
    Additional Material: 4 Ill.
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  • 4
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: By the “labeled mitoses” method of Quastler and Sherman and others, the cell cycle of the germinative zone cells of the bullfrog lens epithelium has been characterized. It has been shown that this cycle lasts approximately 83 days with the DNA synthetic phase enduring 100 hours and G2, 11 hours. G1 occupies over 90% of the total time. the duration of mitosis itself has not been precisely determined. the length of the synthetic phase was corroborated by double labeling with c14 and h3-thymidine.When the temperature is raised by 6°c, from 24° to 30° the cycle is compressed by 40%.When the nongerminative, central cells of bullfrog lens epithelium are activated (stimulated to undergo DNA synthesis and mitosis) by injury or through in vitro culture, the length of the cycle also appears to decrease. in the in vitro experiments the generation time, as judged by the period elapsing between two successive bursts of DNA synthesis involving the same cells, amounts to 177-190 hours at 24°c. by raising the temperature to 30°c the time from injury or isolation until the appearance of the first wave of mitosis is reduced by 20%.
    Additional Material: 6 Ill.
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