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  • 1
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Studies were conducted to clarify the relationship between growth rate and disease progression of erythrocytic inclusion body syndrome (EIBS) in artificially infected coho salmon, Oncorhynchus kisutch (Walbaum). In the diseased state, the haematocrit values decreased and the number of erythrocytes with inclusions was higher in faster growing fish. Rapid growth was accompanied by an abundance of immature erythrocytes, which had the greatest incidence of inclusion bodies.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 36 (1989), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Both monoclonal and polyclonal antisera were produced against Ceratomyxa shasta. Ascites containing trophozoites of the parasite was collected from infected fish and used as antigen for immunization of mice. The resulting monoclonal antibodies reacted specifically with trophozoite and sporoblast stages but did not react with C. shasta spores by either indirect fluorescent antibody techniques or in Western blots. This indicates that some C. shasta antigens are specific to certain life stages of the parasite. Polyclonal antiserum was produced in a rabbit by injecting a spore protein electro-eluted from an SDS-polyacrylamide gel. This antiserum reacted with both trophozoites and spores by indirect fluorescent antibody techniques and in Western blots. All antisera were tested for cross-reactivity to trout white blood cells, a contaminant of the ascites, and to other myxosporea. Two monoclonal antibodies reacted with white blood cells and myxosporea of the genera Sphaerospora and Myxobilatus. One hybridoma produced antibodies of high specificity for C. shasta pre-spore stages. This is the first report of a monoclonal antibody produced against a myxosporean parasite.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Steelhead trout, Oncorhynchus mykiss (Walbaum), fry were experimentally infected with infectious haematopoietic necrosis virus (IHNV) Round Butte 1983 (Type 1). Fry were sampled daily, before and during the epizootic. Fish tissues were tested for infectious virus by tissue culture assay and for IHNV nucleocapsid protein by alkaline phosphatase immunohistochemistry (APIH). The progression of virus through the tissues was followed by APIH until the fourteenth day. Viral infection progressed from two major sites: from the gills into the circulatory system; and from the oral region into the gastrointestinal tract and then into the circulatory system. Once in the blood, virus was disseminated to virtually every organ. Progression of IHNV within and between organs is discussed.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. The tissue response of Salmo gairdneri Richardson, against the myxosporean parasite. Ceratomyxa shasta (Noble), was investigated using histological techniques, scanning electron microscopy and immunological methods. The progress of infection in C. shasta-susceptible and resistant steelhead and rainbow trout was examined by standard histological techniques and by indirect fluorescent antibody methods using monoclonal antibodies directed against C. shasta antigens. Trophozoite stages were first observed in the posterior intestine and there was indication that resistance was due to the inability of the parasite to penetrate this tissue rather than to an inflammatory response. Examination of a severely infected intestine by scanning electron microscopy showed extensive destruction of the mucosal folds of the posterior intestine. Western blotting and indirect fluorescent antibody techniques were used to investigate the immunological component of the host response. No antibodies specific for C. shasta were detected by either method.
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  • 5
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 20 (1997), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The variation of virulence of Renibacterium salmoninarum, the causative agent of bacterial kidney disease (BKD) in salmonid fish, was studied by infecting rainbow trout, Oncorhynchus mykiss (Walbaum), with two isolates (strains 325 and 932) from diseased Atlantic salmon, Salmo salar L., and one isolate (strain 4366) from an apparently healthy Atlantic salmon. Coho salmon, Oncorhynchus kisutch (Walbaum), were injected with the strain 932 to estimate difference in fish species resistance. Fish were removed by random sampling for other study purposes, a study design possible with analysis of lifetime distributions incorporating both sampling-, death- and survival-times. At the end of the experiment, the rainbow trout infected with strains 325, 932 and 4366 had a survival probability of 33%, 51% and 72%, respectively. The coho salmon infected with strain 932 had a 26% survival probability. The strain differences were significant according to the log-rank test, and the risk ratio between the strains ranged from 1·8 to 5·4. The strain from the apparently healthy fish was least virulent. The survival of the fish species was different over time. Rainbow trout were more likely to die early in the time course, but high numbers of coho died later, resulting in an overall risk of mortality of 1·4 in favour of rainbow trout. Differences in virulence may reflect changed selective pressure on R. salmoninarum when introduced from feral stocks into the environment of fish farms.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 9 (1986), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 7 (1984), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. The histopathology associated with naturally acquired vibriosis in chum salmon, Oncorhynchus keta (Walbaum), fingerlings caused by Vibrio anguillarum was compared with that caused by infection with Vibrio ordalii. Pathogenesis of the two forms was found to be different. Bacteraemia caused by V. anguillarum occurred in the early stages with pronounced histopathological changes in blood, loose connective tissue, kidney, spleen, gills and posterior gastrointestinal tract. Bacterial cells appeared uniformly dispersed throughout the affected tissues but were most abundant in the blood. With V. ordalii, bacteraemia developed only in late stages of the disease and the concentration of bacterial cells per ml of blood was less than in the V. anguillarum infection by a factor of 102–103. Tissues with most pronounced changes were skeletal and cardiac muscle, anterior and posterior gastrointestinal tract and the gills. Vibrio ordalii observed in the tissues was not evenly dispersed but was present within tissue as colonies or aggregates of cells. The differences in pathology observed in naturally infected chum salmon were produced experimentally with each pathogen by waterborne exposure of chum; coho, Oncorhynchus kisutch (Walbaum); and chinook salmon, Oncorhynchus tshawytscha (Walbaum). Severe decreases in circulating leucocytes accompanied bacteraemia caused by either bacterial species.
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  • 9
    Publication Date: 2016-06-28
    Description: Hilly regions along the Western Carpathian–Pannonian border are phytogeographically important, but their vegetation history remains largely unknown. We analysed two peat cores of Late Glacial origin from a bog woodland in the Malé Karpaty Mts (SW Slovakia) using plant macrofossil, pollen, peat chemistry and charcoal analyses to trace local successional patterns, regional vegetation development and occurrence of rare species. The small distance between the two profiles situated within homogeneous vegetation enabled us to explore small-scale differences in local vegetation history. The sediment started to accumulate at the end of the Allerød (ca. 12950 cal. yr BP), when a shallow oligotrophic/mesotrophic lake with macrophytes developed. Open pine-birch forests dominated in the landscape. During the early Holocene, the lake was infilled, mire vegetation appeared and broad-leaved forests spread in the surroundings. Two fire events indicated by increases in number of macroscopic charcoal particles were recorded. The first one, which occurred at the end of the Late Glacial, was found only in one of the profiles, while the second one affected entire mire and probably caused a hiatus spanning the middle and late Holocene. Fagus started to spread no later than 5800 cal. yr BP. Open mire vegetation reappeared after the fire (ca. 400 cal. yr BP). During the 19th century, the mire was overgrown by a birch bog woodland. The two profiles showed basically the same successional patterns, but some local events and occurrences of rare species ( Potamogeton alpinus , Potamogeton praelongus , Scorpidium scorpioides and Pleurospermum austriacum ) were traced only in one of them.
    Print ISSN: 0959-6836
    Electronic ISSN: 1477-0911
    Topics: Geography , Geosciences
    Published by Sage
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  • 10
    Publication Date: 2013-03-07
    Description: A bstract : The Carpathian Mountains across Slovakia and Romania are home of several ice caves located at elevations between 700 and 1,200 m above sea level (asl). Although the mean surface annual temperature is above the freezing point, perennial ice deposits are common in caves and shafts with certain morphologies (large entrances followed by steep vertical or downward-sloping passages), into which the dense cold winter air sinks and remains trapped all year round. A particular type of cave pearls (cryogenic cave pearls, CCPs) occur in spatially restricted accumulations or extensive pearl fields (layers locally up to 0.5 m in thickness) within the scree covering the cave floor in the periglacial zone of these caves. The temperature in the periglacial zone oscillates around the freezing point, promoting seasonal ice formation. A similar type of pearl is observed in the entrance section of other caves that experience temporary freezing conditions during the cold season. When compared to pearls of non-iced caves, those from ice caves always occur at sites where liquid water cannot accumulate. CCP formation in nests with drips or in cave pools surrounded by rimstone dams is therefore excluded. Freezing–thawing processes are responsible for moving the pearls, preventing their cementation to the floor or between them. Results of U-series and radiocarbon dating indicate that the pearls are Holocene in age, with their growth continuing into the present. Pearls show high porosity, ranging from 7.6 to 22.6%. In the center they frequently contain radial aggregates of larger carbonate crystals, and their concentric layering is less obvious compared to normal cave pearls. In addition, polygonal pearls and pearl aggregates are common. Stable carbon and oxygen isotope study indicates the formation of an initial crystal aggregate by cryogenic precipitation during freezing of drip water. After that, pearls grow by addition of new carbonate layers, either at water freezing conditions or at temperature above 0°C. Overall, the pearl isotope data plot along a mixing line in the 13 C vs. 18 O diagram, connecting the field of normal speleothems of non-iced caves of the area, with the field of fine-grained cryogenic carbonate powder, formed in the glaciated parts of the ice caves. Seasonal water freezing and frost action in the cave periglacial zone is a necessary condition for the formation of these pearls.
    Print ISSN: 1527-1404
    Topics: Geosciences
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