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  • 1
    Electronic Resource
    Electronic Resource
    A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen (1997)
    Springer
    Plant cell reports 16 (1997), S. 474-478 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cassava (Manihot esculenta Crantz) ; Cryopreservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: pre-cultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen, the shoot tips were removed, rapidly warmed, and recultured sequentially in three recovery media. After 2 weeks, the regeneration of frozen shoot tips was completed. Genotypes with a low response were identified. Their response was attributed to the effects of pre- and post-freezing steps. Refining the methodology led to a consistent 50–70% plant recovery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092769
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  • 2
    Electronic Resource
    Electronic Resource
    A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen (1997)
    Springer
    Plant cell reports 16 (1997), S. 474-478 
    Details
    ISSN: 1432-203X
    Keywords: Cassava (Manihot esculenta Crantz) ; Cryopreservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: pre-cultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen, the shoot tips were removed, rapidly warmed, and recultured sequentially in three recovery media. After 2 weeks, the regeneration of frozen shoot tips was completed. Genotypes with a low response were identified. Their response was attributed to the effects of pre and post-freezing steps. Refining the methodology led to a consistent 50–70% plant recovery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092769
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  • 3
    Electronic Resource
    Electronic Resource
    Transgenic plants of cassava (Manihot esculenta) with resistance to Basta obtained by Agrobacterium-mediated transformation (2000)
    Springer
    Plant cell reports 19 (2000), S. 339-344 
    Details
    ISSN: 1432-203X
    Keywords: Key words Manihot esculenta ; bar ; ppt/Basta-resistance ; useful transgenes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive for PCR- and/or Southern-based detection of the nptII gene. Somatic-embryo-derived cotyledons were used as source of explants. A non-disarmed Agrobacterium strain (CIAT 1182) was used to transfer the genes of interest into cassava cultivar MPer183. Greenhouse tests of resistance to Basta (Hoechst) showed three plant lines with different levels of tolerance to the herbicide. Based on Southern tests of transgenesis, the transformation efficiency was 1%. The results constitute the first report of the bar gene conferring herbicide resistance to cassava plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050737
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  • 4
    Electronic Resource
    Electronic Resource
    Regeneration of isolated mesophyll and cell suspension protoplasts to plants in Stylosanthes guianensis. A tropical forage legume (1986)
    Springer
    Plant cell reports 5 (1986), S. 174-177 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts were isolated from leaf mesophyll and cell suspensions of two accessions of Stylosanthes guianensis (Aubl.), a tropical forage legume. When cultured in VKM liquid culture medium, both types of protoplasts divided at a rate of 4–8%, and subsequently formed cell colonies. Protoplast-derived calluses produced numerous shoots when transferred to regeneration medium. Regenerated shoots could be easily rooted, and plantlets were transferred to soil. The effects of several factors on the efficiency of this protoplast system have been investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269111
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  • 5
    Electronic Resource
    Electronic Resource
    Variability of chloroplast DNA and nuclear ribosomal DNA in cassava (Manihot esculenta Crantz) and its wild relatives (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 719-727 
    Details
    ISSN: 1432-2242
    Keywords: Cassava ; Wild Manihot species Chloroplast DNA ; Nuclear ribosomal DNA ; Phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chloroplast DNA (cp) and nuclear ribosomal DNA (rDNA) variation was investigated in 45 accessions of cultivated and wild Manihot species. Ten independent mutations, 8 point mutations and 2 length mutations were identified, using eight restriction enzymes and 12 heterologous cpDNA probes from mungbean. Restriction fragment length polymorphism analysis defined nine distinct chloroplast types, three of which were found among the cultivated accessions and six among the wild species. Cladistic analysis of the cpDNA data using parsimony yielded a hypothetical phylogeny of lineages among the cpDNAs of cassava and its wild relatives that is congruent with morphological evolutionary differentiation in the genus. The results of our survey of cpDNA, together with rDNA restriction site change at the intergenic spacer region and rDNA repeat unit length variation (using rDNA cloned fragments from taro as probe), suggest that cassava might have arisen from the domestication of wild tuberous accessions of some Manihot species, followed by intensive selection. M. esculenta subspp flabellifolia is probably a wild progenitor. Introgressive hybridization with wild forms and pressures to adapt to the widely varying climates and topography in which cassava is found might have enhanced the crop's present day variability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223711
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  • 6
    Electronic Resource
    Electronic Resource
    Interspecific hybridization between common and tepary beans: increased hybrid embryo growth, fertility, and efficiency of hybridization through recurrent and congruity backcrossing (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 324-331 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus acutifolius ; Phaseolus vulgaris ; Common and tepary beans ; Interspecific hybridization ; Congruity and recurrent backcrosses ; In-vitro embryo culture ; Gene introgression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cultivated common bean (Phaseolus vulgaris L.) and tepary bean (Phaseolus acutifolius A. Gray) genotypes possessing desirable agronomic traits were hybridized. The F1 hybrids were backcrossed twice with the common bean (i.e., recurrent backcrossing). Also, alternate backcrosses with common and tepary beans (i.e., congruity backcrossing) were carried out. Embryo culture was necessary for all initial interspecific crosses, and its requirement was proportionally lower when the common bean was used as the recurrent parent and as the last parent of congruity backcrosses. Modification of the embryo culture technique was necessary to produce congruity hybrids. Effects of both tepary and common bean genotypes on the success rate of hybridization were observed. Tepary accession G 40001 and common bean cultivar ICA Pijao facilitated interspecies hybridization. Growth of hybrid embryos before rescue, recovery of mature hybrid plants, and the vigor and fertility of F1 hybrids all increased with increased recurrent and congruity backcrosses and intermatings between male-sterile F1 and selected fertile F2 plants of the third and fifth congruity backcrosses. Introgression of tepary genes was verified by means of seed protein electrophoretic analysis and morphological markers. The results suggest that congruity backcrossing can help to gradually reduce or overcome P. vulgaris x P. acutifolius hybridization barriers such as genotype incompatibility, early embryo abortion, hybrid sterility, and lower frequencies of hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223640
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  • 7
    Electronic Resource
    Electronic Resource
    Interaccession variation of electrophoregrams of cotyledon protein of field bean (Phaseolus vulgaris L.) (1988)
    Springer
    Euphytica 39 (1988), S. 109-111 
    Details
    ISSN: 1573-5060
    Keywords: Phaseolus vulgaris ; field bean ; cotyledon protein ; electrophoregrams ; interaccession variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Polyacrylamide gel electrophoresis at acidic pH showed substantive qualitative pattern difference among accessions that had been classified into one cultivar by conventional taxonomic procedures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039861
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  • 8
    Electronic Resource
    Electronic Resource
    Identification of cultivars of pasture legumes (Centrosema macrocarpum, C. pubescens and C. sp.n.) by acid polyacrylamide gel electrophoresis of cotyledons storage proteins (1988)
    Springer
    Euphytica 39 (1988), S. 105-107 
    Details
    ISSN: 1573-5060
    Keywords: Centrosema macrocarpum ; Centrosema pubescens ; cultivar identification ; Acid PAGE ; Storage proteins ; pasture legume
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An electrophoretic procedure was developed for storage proteins which can discriminate cultivars of forage legumes, Centrosema macrocarpum, C. pubescens and C. sp.n. Proteins extracted from cotyledons were separated in 10% polyacrylamide gel containing 10% sucrose. Electrophoretic patterns are presented to illustrate the results that can be obtained with the procedure described. Cultivars of all three species were distinguishable based upon the variation in their acid soluble seed proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039860
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  • 9
    Electronic Resource
    Electronic Resource
    Identification of cultivars of the forage legume Pueraria phaseoloides by electrophoretic patterns of storage proteins (1989)
    Springer
    Euphytica 41 (1989), S. 71-73 
    Details
    ISSN: 1573-5060
    Keywords: forage legume ; Pueraria phaseoloides cultivar identification ; electrophoresis ; acid PAGE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A polyacrylamide gel electrophoresis procedure was developed for discriminating cultivars of the forage legume Pueraria phaseoloides on the basis of the patterns of cotyledon proteins. The genotypic marker proteins were extracted with 5M acetic acid and electrophoresed at pH 3.1 in aluminum lactate buffer. The procedure gave highly reproducible discrimination of ten selected cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022413
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  • 10
    Electronic Resource
    Electronic Resource
    Identification of cultivars of Stylosanthes capitata Vog. by polyacrylamide gel electrophoresis of seed proteins (1988)
    Springer
    Euphytica 37 (1988), S. 117-119 
    Details
    ISSN: 1573-5060
    Keywords: Stylosanthes capitata ; pasture legume ; cultivar identification ; polyacrylamide gel electrophoresis ; seed proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method was developed for identification of cultivars of the pasture legume, Stylosanthes capitata Vog., using electrophoretic patterns of seed proteins in polyacrylamide gels as the genotypic markers. The method can be used for accurate identification of cultivars in germplasm banks, in selecting parents for development of new varieties, and in registering new cultivars for proprietary purposes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036848
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