ISSN:
1550-7408
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
THE role of lysosomal enzymes in intracellular digestion is now well established [11]. Most often we think of lysosomal hydrolases in catabolism of endogenous or foreign material taken up by endocytosis. There is however, a number of reports dealing with the release of acid hydrolases into the extracellular fluid in a variety of eukaryote cells. These cells range from Saccharomyces cerevisiae [15], Dictyostelium discoideum [10], Leishmania donovani [20], Acanthamoeba castellani [22], Entamoeba histolytica [12, 31], and species of Tetrahymena [1–3, 6] to mammalian cells in culture [49]. Concerning the latter, fibroblasts and hepatocytes in culture release acid hydrolases to the extracellular medium, but only if the synthesis of a specific recognition marker is impaired in the cells. This marker (man-nose-6-phosphate) is used for receptor mediated segregation of lysosomal enzymes into the lysosomal compartments. If the receptor or the marker are lacking, the hydrolases fail to enter the lysosomal compartment, and are secreted in immature form together with molecules belonging to the constitutive secretory pathway of the cells [8, 49]. Such a release of acid hydrolases seems to occur spontaneously from mammalian osteoclasts [4]. Macrophages, on the other hand, need a specific stimulation for their release process [40]. In lower eukaryotes the release may
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1550-7408.1989.tb05530.x
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