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  • 1
    Electronic Resource
    Electronic Resource
    Structure determination of tolaasin, an extracellular lipodepsipeptide produced by the mushroom pathogen, Pseudomonas tolaasii Paine (1991)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 113 (1991), S. 2621-2627 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00007a040
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  • 2
    Electronic Resource
    Electronic Resource
    Comparison of Borrelia isolated from UK foci of Lyme disease (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 130 (1995), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Restriction endonuclease digestion of linear borrelial chromosomal DNA showed that three isolates of UK Lyme disease spirochaetes differed markedly from each other and from published data for other isolates from North America and continental Europe. Analysis of linear plasmid bands revealed that UK isolates differed from each other in the number and sizes of the plasmids in isolates from different foci of UK Lyme disease. Fatty acid analysis (of fatty acid methyl ester (FAME) profiles) showed the UK isolates clustering together with the relapsing fever spirochaetes, Borrelia turicatae and Borrelia parkeri. These data are discussed in respect of current knowledge of Lyme borreliosis in the UK.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07712.x
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  • 3
    Electronic Resource
    Electronic Resource
    Physical and genetic map of the Pseudomonas fluorescens SBW25 chromosome (1996)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 19 (1996), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Pseudomonas fluorescens is a saprophytic bacterium commonly isolated from soil, water, and the surfaces and tissues of plants and animals. The species has important applications in biotechnology because it can enhance plant growth and protect crops against disease. A complete physical map of the 6.63 Mbp P. fluorescens SBW25 chromosome was constructed using data obtained from combinations of one- and two-dimensional electrophoresis of completely or partially digested chromosomal DNA with end labelling. In total, 139 restriction sites (15 PacI, 53 SpeI, 71 XbaI) were placed on the physical map and complete maps of the circular chromosome were obtained for both PacI and SpeI; only XbaI fragments linking SpeI fragments were positioned. The average resolution of restriction sites was 48 kbp. A genetic map was derived from the physical map by Southern hybridization and 31 genes were positioned including oriC, rDNA operons (rnnA–E), recAgacA and pyvD
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1996.391926.x
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  • 4
    Electronic Resource
    Electronic Resource
    The indigenous Pseudomonas plasmid pQBR103 encodes plant-inducible genes, including three putative helicases (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 51 (2004), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Plasmid pQBR103 (∼400 kb) is representative of many self-transmissible, mercury resistant plasmids observed in the Pseudomonas community colonising the phytosphere of sugar beet. A promoter trapping strategy (IVET) was employed to identify pQBR103 genes showing elevated levels of expression on plant surfaces. Thirty-seven different plant-inducible gene fusions were isolated that were silent in laboratory media, but active in the plant environment. Three of the fusions were to DNA sequences whose protein products show significant homology to DNA-unwinding helicases. The three helicase-like genes, designated helA, helB and helC, are restricted to a defined group of related Pseudomonas plasmids. They are induced in both the root and shoot environments of sugar beet seedlings. Sequence analysis of the three plasmid-encoded helicase-like genes shows that they are phylogenetically distinct and likely to have independent evolutionary histories. The helA gene is predicted to encode a protein of 1121 amino acids, containing conserved domains found in the ultraviolet (UV) resistance helicase, UvrD. A helA knockout mutant was constructed and no phenotypic changes were found with plasmid-conferred UV resistance or plasmid conjugation. The other 34 fusions are unique with no homologues in the public gene databases, including the Pseudomonas genomes. These data demonstrate the presence of plant responsive genes in plasmid DNA comprising a component of the genomes of plant-associated bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsec.2004.07.006
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  • 5
    Electronic Resource
    Electronic Resource
    Short-term community dynamics in the phyllosphere microbiology of field-grown sugar beet (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 16 (1995), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Short-term fluctuations in the number of culturable microorganisms and the composition of the bacterial community colonising the phyllosphere of field grown sugar beet were determined at 6 h intervals over a 42 h period. The number of culturable bacteria and Erwinia were found to fluctuate diurnally, increasing in size overnight and declining during daylight hours. In contrast, the number of Arthrobacter and yeasts were constant throughout the study, even after rainfall. Over most of the study the number of filamentous fungi and pseudomonads decreased. The short-term fluctuations in bacterial numbers were similar in magnitude to those detected over most of the previous season. No significant variation was observed in microbial numbers between samples taken from three locations within the field. Fatty acid analysis of isolated bacteria revealed the community to be similar in composition to that detected during the same period the previous year and indicated no significant short-term temporal or spatial variation. To limit the effects of diurnal fluctuations on the number of culturable bacteria detected, it is recommended that in long-term studies, samples be collected at the same time of day on each occasion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1995.tb00284.x
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  • 6
    Electronic Resource
    Electronic Resource
    Biofilm formation at the air–liquid interface by the Pseudomonas fluorescens SBW25 wrinkly spreader requires an acetylated form of cellulose (2003)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 50 (2003), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The wrinkly spreader (WS) genotype of Pseudomonas fluorescens SBW25 colonizes the air–liquid interface of spatially structured microcosms resulting in formation of a thick biofilm. Its ability to colonize this niche is largely due to overproduction of a cellulosic polymer, the product of the wss operon. Chemical analysis of the biofilm matrix shows that the cellulosic polymer is partially acetylated cellulose, which is consistent with predictions of gene function based on in silico analysis of wss. Both polar and non-polar mutations in the sixth gene of the wss operon (wssF ) or adjacent downstream genes (wssGHIJ ) generated mutants that overproduce non-acetylated cellulose, thus implicating WssFGHIJ in acetylation of cellulose. WssGHI are homologues of AlgFIJ from P. aeruginosa, which together are necessary and sufficient to acetylate alginate polymer. WssF belongs to a newly established Pfam family and is predicted to provide acyl groups to WssGHI. The role of WssJ is unclear, but its similarity to MinD-like proteins suggests a role in polar localization of the acetylation complex. Fluorescent microscopy of Calcofluor-stained biofilms revealed a matrix structure composed of networks of cellulose fibres, sheets and clumped material. Quantitative analyses of biofilm structure showed that acetylation of cellulose is important for effective colonization of the air–liquid interface: mutants identical to WS, but defective in enzymes required for acetylation produced biofilms with altered physical properties. In addition, mutants producing non-acetylated cellulose were unable to spread rapidly across solid surfaces. Inclusion in these assays of a WS mutant with a defect in the GGDEF regulator (WspR) confirmed the requirement for this protein in expression of both acetylated cellulose polymer and bacterial attachment. These results suggest a model in which WspR regulation of cellulose expression and attachment plays a role in the co-ordination of surface colonization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2003.03670.x
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  • 7
    Electronic Resource
    Electronic Resource
    Evolution of species interactions in a biofilm community (2007)
    [s.l.] : Nature Publishing Group
    Nature 445 (2007), S. 533-536 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Biofilms are spatially structured communities of microbes whose function is dependent on a complex web of symbiotic interactions. Localized interactions within these assemblages are predicted to affect the coexistence of the component species, community structure and function, but there have ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature05514
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  • 8
    Electronic Resource
    Electronic Resource
    Unity from conflict (2007)
    [s.l.] : Nature Publishing Group
    Nature 446 (2007), S. 616-616 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The transition from unicellular to multicellular life brought with it division of labour, development and a panoply of animal and plant forms. But despite its pivotal role in the history of life, the evolution of multicellularity is poorly understood. Watching the de novo emergence of primordial ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/446616a
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  • 9
    Electronic Resource
    Electronic Resource
    Immigration history controls diversification in experimental adaptive radiation (2007)
    [s.l.] : Nature Publishing Group
    Nature 446 (2007), S. 436-439 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Diversity in biological communities is a historical product of immigration, diversification and extinction, but the combined effect of these processes is poorly understood. Here we show that the order and timing of immigration controls the extent of diversification. When an ancestral ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature05629
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  • 10
    Electronic Resource
    Electronic Resource
    The role of parasites in sympatric and allopatric host diversification (2002)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 420 (2002), S. 496-499 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Exploiters (parasites and predators) are thought to play a significant role in diversification, and ultimately speciation, of their hosts or prey. Exploiters may drive sympatric (within-population) diversification if there are a variety of exploiter-resistance strategies or fitness costs ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature01164
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