Publication Date:
2000-03-24
Description:
We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9- megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99. 99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Myers, E W -- Sutton, G G -- Delcher, A L -- Dew, I M -- Fasulo, D P -- Flanigan, M J -- Kravitz, S A -- Mobarry, C M -- Reinert, K H -- Remington, K A -- Anson, E L -- Bolanos, R A -- Chou, H H -- Jordan, C M -- Halpern, A L -- Lonardi, S -- Beasley, E M -- Brandon, R C -- Chen, L -- Dunn, P J -- Lai, Z -- Liang, Y -- Nusskern, D R -- Zhan, M -- Zhang, Q -- Zheng, X -- Rubin, G M -- Adams, M D -- Venter, J C -- New York, N.Y. -- Science. 2000 Mar 24;287(5461):2196-204.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Celera Genomics, Inc., 45 West Gude Drive, Rockville, MD 20850, USA. Gene.Myers@celera.com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10731133" target="_blank"〉PubMed〈/a〉
Keywords:
Algorithms
;
Animals
;
Chromatin/genetics
;
*Computational Biology
;
Contig Mapping
;
Drosophila melanogaster/*genetics
;
Euchromatin
;
Genes, Insect
;
*Genome
;
Heterochromatin/genetics
;
Molecular Sequence Data
;
Physical Chromosome Mapping
;
Repetitive Sequences, Nucleic Acid
;
*Sequence Analysis, DNA
;
Sequence Tagged Sites
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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