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  • 1
    Electronic Resource
    Electronic Resource
    Apium Wild Species: Novel Sources for Resistance to Late Blight in Celery (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 102 (1989), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Screening of 144 varieties of celery A. graveolens L. for late blight (Septoria apiicola Speg.) resistance resulted in the detection of significant differences for disease reaction. However, each accession displayed greater than 25 % leaf necrosis area due to the disease pathogen. Hence the magnitude of infection in the least susceptible plants was still too great to use them as a valuable source for resistance. The wild species A. chilense and A. panul exhibited a few discrete yellow spots with very little, if any, pycnidial development. This type of resistance was not observed in any accession of celery screened. The degree of resistance was intermediate in the F1 hybrids between A. graveolens × A. chilense and A. graveolens×A. panul, indicating incomplete dominance. The resistance to late blight found in these wild species and their ability to cross with celery provides a novel germplasm source for breeding celery lines with improved resistance to late blight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1989.tb01262.x
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  • 2
    Electronic Resource
    Electronic Resource
    Dissection of the Brassica nigra Genome by Monosomic Addition Lines (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 105 (1990), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Hyperploid derivatives of Diplotaxis erucoides × Brassica nigra hybrids were used to extract seven out of the eight possible monosoinic addition lines for B. nigra (genome B). The fertility and transmission of the lines varied depending on the added chromosome. However, these parameters were high enough to assure the maintenance of the addition lines. Although no phenotypic changes were observed, the plants carrying extra chromosomes were slower in development than diploid D. emcoides. Each of the B. nigra chromosomes was recognizable cytologically by size and heterochromatin distribution. Seven of these were characterized by a series of isozymes and RFLP markers. Ribosotnal DNA sequences were detected in two independent B. nigra chromosomes. Two probes disclosed fragments dispersed in more than one chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1990.tb01198.x
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic analysis and nomenclature for seven isozyme systems in Brassica nigra, B. oleracea and B. campestris (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: General criteria for the assignment of names to enzyme systems, regions of activity, isozyme loci and allozymes have been lacking in crucifer species. This paper proposes a standard nomenclature for seven isozyme systems in the three diploid species of U's triangle: Brassica nigra, B. oleracea and B. campestris. Gel/electrode buffers, which provided the best resolution for seven isozyme systems, acid phosphatase (APS), aconitase (ACO), leucine aminopeptidase (LAP), 6-phosphogluconate dehydrogenase (6 PGD), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), and triosephosphate isomerase (TPI), were proposed as standards. Isozyme genetic analysis was determined for B. oleracea and B. campestris from previous studies and by segregation of selfed progenies of heterozygous B. nigra plants. Several populations were studied and 148 allozymes at the 18 loci observed were described for the three species. Their relative mobility was studied using a pure line of oilseed rape as reference. The comparison of the different alleles within and between the species is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00839.x
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  • 4
    Electronic Resource
    Electronic Resource
    Correlation between N 1s core level x-ray photoelectron and x-ray absorption spectra of amorphous carbon nitride films (2000)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 77 (2000), S. 803-805 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: This work presents a comparative analysis of the N 1s core level spectra, as measured by x-ray photoelectron spectroscopy (XPS) and x-ray absorption spectroscopy (XAS), of amorphous CNx films which gives evidence of the existing correlation between the different components that constitute the respective spectra. After annealing, the contribution of XPS at 399.3 eV and the components of XAS at 399.6 and 400.8 eV are clearly enhanced. They are assigned to sp2 with two neighbors and to sp states of nitrogen. In addition, the XPS component at 401.3 eV is related to the XAS feature at 402.0 eV and has been assigned to sp2 nitrogen bonded to three carbon neighbors. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1306636
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  • 5
    Electronic Resource
    Electronic Resource
    Characterization of carbon nitride thin films prepared by dual ion beam sputtering (1996)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 69 (1996), S. 764-766 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Carbon nitride thin films obtained by dual ion beam sputtering have been investigated by electron energy-loss spectroscopy (EELS), transmission electron microscopy, and Fourier transform infrared spectroscopy. The nitrogen content in the films depends on deposition conditions. A maximum value of N/C=0.8 has been achieved. A new peak at 286.7 eV energy loss in the C K-edge EELS spectra has been assigned to C=N bonds with C in the sp2 hybridization state. In addition, experimental evidences are presented of the formation of β-C3N4 crystallites embedded in a layer of a polymer like CNx amorphous phase. An evaluation of the experimental parameters that lead to the highest N content in the films is also included. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.117884
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  • 6
    Electronic Resource
    Electronic Resource
    Mapping of Molecular Markers on Brassica B-Genome Chromosomes Added to Brassica napus (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 108 (1992), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Using interspecific hybridization among various Brassica species, B-genome chromosomes from different sources of Brassica, i.e. B. nigra (BB, 2n = 18), B. carinata (BBCC), 2n = 34) and B. juncea (AABB, 2n = 36) were transferred into the Canadian variety ‘Andor’ of B. napus. Monosomic addition lines were selected (AACC + 1B, 2n = 39) by cytological control. For characterization of the alien chromosomes, series of isozymes, RFLPs and RAPD markers were employed. This permitted the identification of a total of 39 lines representing seven of the eight B-genome chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1992.tb00137.x
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  • 7
    Electronic Resource
    Electronic Resource
    Characterization of Zr thin films grown by dual ion-beam sputtering
    Amsterdam : Elsevier
    Vacuum 45 (1994), S. 1039-1041 
    Details
    ISSN: 0042-207X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0042-207X(94)90017-5
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  • 8
    Electronic Resource
    Electronic Resource
    Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Significant heterosis for seed yield in oilseed rape has created interest in the development of hybrid cultivars. The DNA-based marker protocol, sequence-related amplified polymorphism (SRAP) was used to determine genetic diversity among oilseed rape maintainer and restorer lines. This measure was used in an attempt to establish an association between genetic distance and heterosis in hybrids for various agronomic traits. A total of 118 polymorphic loci were generated by 18 SRAP primer combinations. Based on the polymorphism generated by the markers, calculated similarity index values ranged from 0.46 to 0.97. Cluster analysis grouped 10 maintainer and 12 restorer lines into three groups, with the exception of two maintainer lines, PM5 and PM9, which fell outside these groups. The grouping of the lines was largely in agreement with the available pedigree data on their origin and agronomic performance. Analysis of variance among inbred lines and their resulting F1 hybrids over two locations revealed significant differences for plant height, days to maturity and seed yield, but not for oil content. Substantial mid-parent heterosis was observed only for seed yield, and ranged from 26% to 169%. All hybrids surpassed their respective inbred lines for this trait, except for a single cross combination of related lines. In general, crosses of lines located in different clusters yielded more than those from the same clusters. Regression analysis revealed a statistically significant relationship between the genetic distance of the parents and seed yield in their hybrid, and their derived mid-parent and high-parent heterosis. The correlation coefficient between genetic distance and yield (0.64) indicated a moderately strong relationship, so it is possible that some of the SRAP markers might be linked to quantitative trait loci for seed yield.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00636.x
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  • 9
    Electronic Resource
    Electronic Resource
    SCAR and RAPD markers associated with 18-carbon fatty acids in rapeseed, Brassica napus (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 118 (1999), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Breeding rapeseed for enhanced oil quality includes the development of varieties with low linolenic acid content. The breeder also aims to develop varieties with a high linoleic acid content because of its nutritional value. Restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers have been developed for linolenic acid content, but they are not best suited for a direct application in marker-assisted selection. The RFLP technique is too complex and time-consuming and RAPD markers lack codominance, precluding the distinction of homozygous from heterozygous individuals. In this report the conversion of a RAPD marker to a codominant sequence characterized amplified region (SCAR) marker named L1L9 is described. One of the alleles consisting of an 899 bp fragment (allele A), is associated with low linolenic acid content. The other allele consists of an 641 bp fragment (allele B) and is associated with high linolenic acid content. This marker explains approximately 25% of the genetic variation for this trait. Linkage analysis in the mapping population indicates that the SCAR marker probably tags an ω-3 desaturase gene in B. napus. Two RAPD markers were found to be associated with oleic/linoleic acid content. Markers M14-350 and I06-650 explained approximately 10% and 7% of the genetic variation for linoleic acid content, respectively. These two markers were found linked at 12.3cM in the segregating B. napus F2 progeny used for mapping. All the markers reported in this paper should be useful in breeding programmes for developing high linoleic and low linolenic acid rapeseed varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.1999.118002145.x
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  • 10
    Electronic Resource
    Electronic Resource
    Genetic and physical mapping in Brassica diploid species of a gene cluster defined in Arabidopsis thaliana (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 298-306 
    Details
    ISSN: 1617-4623
    Keywords: Key words Genome ; Colinearity ; RFLPs ; Chromosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We report the genetic and physical analysis by pulse field gel electrophoresis (PFGE) in three Brassica diploid genomes for a cluster of five genes characterized in a selected segment of 15 kb on chromosome 3 of Arabidopsis thaliana, encoding a Bradyrhizobium CycJ homologue (At1), a rat p67 translation factor homologue (At2), an Em-like (early methionine) protein (At3), chlorophyll synthase (At4) and a yeast Sac1 homologue (A5). The Arabidopsis gene array was found to be conserved on a single linkage group in each of the Brassica genomes. However, partial complexes were found to be duplicated in other chromosome segments on the same or other linkage groups. Some of the At genes, which could not be genetically mapped because of lack of polymorphism, were assigned to their respective linkage groups by physical mapping. The presence of multiple copies of the A. thaliana gene cluster in the three Brassica genomes further establishes their complex nature, which results from extensive duplication and chromosomal rearrangement. In general, genetic distances between the At genes agreed with values expected for the physical distances determined in Brassica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172520
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