Call number:
9783319974545 (e-book)
Description / Table of Contents:
Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available.
Type of Medium:
12
Pages:
1 Online-Ressource (xi, 368 Seiten)
,
Illustrationen, Diagramme
Edition:
Second Edition
ISBN:
9783319974545
,
978-3-319-97454-5
URL:
Ebook (access only within the AWI network)
DOI:
10.1007/978-3-319-97454-5
Language:
English
Note:
Contents
1 Introduction and Historical Perspective / Robert L. Price and W. Gray (Jay) Jerome
2 The Theory of Fluorescence / W. Gray (Jay) Jerome
3 Fluorescence Microscopy / W. Gray (Jay) Jerome and Robert L. Price
4 Specimen Preparation / W. Gray (Jay) Jerome, John Fuseler, Caleb A. Padgett, and Robert L. Price
5 Labeling Considerations for Confocal Microscopy / R. M. Albrecht and J. A. Oliver
6 Digital Imaging / W. Gray (Jay) Jerome
7 Confocal Digital Image Capture / W. Gray (Jay) Jerome
8 Types of Confocal Instruments: Basic Principles and Advantages and Disadvantages / John Fuseler, W. Gray (Jay) Jerome, and Robert L. Price
9 Setting the Confocal Microscope Operating Parameters / Amy E. Rowley, Anna M. Harper, and Robert L. Price
10 3D Reconstruction of Confocal Image Data / Thomas C. Trusk
11 Analysis of Image Similarity and Relationship / Jesse Aaron and Teng-Leong Chew
12 Ethics and Resources / W. Gray (Jay) Jerome and Robert L. Price
Glossary (Terms Are Defined with Respect to Confocal Imaging)
Index
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