ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

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Resolution of Acanthamoeba castellanii chromosomes by pulsed field gel electrophoresis and construction of the initial linkage map (1988)

Rimm, David L. ; Pollard, Thomas D. ; Hieter, Philip

Springer

Chromosoma 97 (1988), S. 219-223

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ISSN: 1432-0886

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract Pulsed field gel electrophoresis has been used to resolve chromosome-sized DNA molecules in fungi and parasites but has not yet been used successfully to examine the chromosomes of other lower eukaryotes used extensively for biochemical research such as Acanthamoeba, Physarum, and Dictyostelium. Here we show an electrophoretic karyotype of the protozoan Acanthamoeba castellanii using orthogonal field alternating gel electrophoresis (OFAGE). There are about 20 small chromosomes ranging in size from 220 kb to 〉2 Mb. We have assembled initial linkage groups assigning all of the cloned Acanthamoeba genes to chromosome-sized DNA molecules. Actin, suggested to have three or more non-allelic genes, maps to at least eight distinct chromosome bands. Two myosin II genes localize to two different chromosomal bands while myosin IB and 18S rRNA map to unresolved larger chromosomes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00292964

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2

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A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complex (2003)

Panchal, Sanjay C ; Kaiser, Donald A ; Torres, Eduardo ; [et al.]

[s.l.] : Nature Publishing Group

Nature structural biology 10 (2003), S. 591-598

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ISSN: 1072-8368

Source: Nature Archives 1869 - 2009

Topics: Biology , Medicine

Notes: [Auszug] Members of the Wiskott-Aldrich syndrome protein (WASP) family link Rho GTPase signaling pathways to the cytoskeleton through a multiprotein assembly called Arp2/3 complex. The C-terminal VCA regions (verprolin-homology, central hydrophobic, and acidic regions) of WASP and its relatives stimulate ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/nsb952

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3

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Proteins as machines (1992)

Pollard, Thomas D.

[s.l.] : Nature Publishing Group

Nature 355 (1992), S. 17-18

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] THIRTEEN different types of protein machine, the driving units that produce macromolecular movement in living organisms, came under scrutiny at the sixth molecular biology symposium at Indiana University late last year*. The upshot is a clearer view of the factors that limit our ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/355017a0

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4

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Dependence of the mechanical properties of actin/α-actinin gels on deformation rate (1987)

Sato, Masahiko ; Schwarz, William H. ; Pollard, Thomas D.

[s.l.] : Nature Publishing Group

Nature 325 (1987), S. 828-830

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] We evaluated the mechanical properties of mixtures of actin and Acanthamoeba a-actinin in a cone-and-plate rheometer using a sinusoidal pattern of low amplitude deformations8'13'15. As described previously15, the small strains (deformations) used do not alter the mechanical properties of ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/325828a0

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5

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Cytoskeleton: Myosin filaments in cytoplasm (1985)

Pollard, Thomas D.

[s.l.] : Nature Publishing Group

Nature 314 (1985), S. 321-321

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] EVERY so often a quiet and contented field of research is awakened by a technical advance that at once changes the conventional wisdom and opens up new avenues for experiments. The paper by Yumura and Fukui in the 14 March issue of Nature1 should have this impact on investigation of myosin ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/314321a0

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6

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Propulsion of organelles isolated from Acanthamoeba along actin filaments by myosin-I (1986)

Adams, Richard J. ; Pollard, Thomas D.

[s.l.] : Nature Publishing Group

Nature 322 (1986), S. 754-756

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] We saw directed motion of vesicles and aggregates of vesicles from Acanthamoeba on the substrate of Nitella actin bundles (Fig. 1). Movements were in the same direction as those of endogeneous algal organelles but substantially slower (Fig. 2b),the average rates of movement being 0.24 0.11 jxms"1 ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/322754a0

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7

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Stimulation of Acanthamoeba actomyosin ATPase activity by myosin-II polymerization (1984)

Kiehart, Daniel P. ; Pollard, Thomas D.

[s.l.] : Nature Publishing Group

Nature 308 (1984), S. 864-866

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Acanthamoeba myosin-II was purified and treated with acid phosphatase to decrease the heavy-chain phosphate level to about 0.8 phosphates per myosin molecule14,15. Myosin-II forms bipolar filaments of two different sizes depending on the solution conditions15. In 0-150 mM KC1 with divalent cation ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/308864a0

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8

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Rich sequel (1990)

Pollard, Thomas D.

[s.l.] : Nature Publishing Group

Nature 346 (1990), S. 621-622

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] THE authors of Molecular Cell Biology have thoroughly revised and beautifully illustrated this second edition, continuing their success in presenting a comprehensive, integrated account of fundamental life processes at the molecular and cellular level. The book is comprehensive in that it covers ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/346621a0

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9

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Binding of myosin I to membrane lipids (1989)

Adams, Richard J. ; Pollard, Thomas D.

[s.l.] : Nature Publishing Group

Nature 340 (1989), S. 565-568

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Although myosin-I was originally isolated as a soluble enzyme, four lines of evidence suggest a membrane association. First, fluorescent-antibody localization4"6 showed Acanth-amoeba myosin-I concentrated near the plasma membrane. Second, the radial spoke between the actin filament core and the ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/340565a0

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10

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The cytoskeleton, cellular motility and the reductionist agenda (2003)

Pollard, Thomas D.

[s.l.] : Nature Publishing Group

Nature 422 (2003), S. 741-745

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Eukaryotic cells depend on cytoskeletal polymers and molecular motors to establish their asymmetrical shapes, to transport intracellular constituents and to drive their motility. Cell biologists are using diverse experimental approaches to understand the molecular basis of cellular movements and to ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/nature01598

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