ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The Zn2+ β-lactamase from Bacteroides fragilis (E.C. 3.5.2.6) was overexpressed in Escherichia coli using an isopropylthiogalactoside-inducible T7 RNA polymerase expression system. Crystallization trials by the hanging-drop vapour-diffusion method have yielded two different crystal forms from two slightly different conditions. Crystals of form I belong to the monoclinic space group C2 with unit-cell dimensions a = 56.03, b = 43.98, c = 105.32 Å, β = 112° and diffracted only up to 4.0 Å. Crystals of form II are orthorhombic, space group P212121 with unit-cell dimensions a = 48.10, b = 98.05, c = 111.76 Å, diffract to at least 2.0 Å and are suitable for high-resolution structural analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444997000966
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