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  • 1
    Publication Date: 2017-06-15
    Description: This study aimed producing a high transparent and colorless cordierite glass–ceramic that is used for a tile glaze with a single firing. Crystallization path of glaze, microstructure development, abrasion performance, microhardness, and the transparency of cordierite glass-ceramic were tested. The results suggested that the high transparency was significantly affected by the number of internal air holes and the crystal scattering loss. The maximum crystalline content of cordierite was nearly 50 wt.% at 1200°C. Due to the high crystallization content of cordierite and the bulk crystallization mechanism, the cordierite crystalline glaze has a higher microhardness and abrasion resistance than non-crystalline glaze. This article is protected by copyright. All rights reserved.
    Print ISSN: 0002-7820
    Electronic ISSN: 1551-2916
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by Wiley
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  • 2
    ISSN: 1432-0983
    Keywords: Pleurotus ; Replicative transformation ; Recombinant plasmids ; DNA methylation ; Plasmid recovery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A transformation system employing selectable resistance to hygromycin B has been developed for the mushroom-forming fungus, Pleurotus ostreatus. Vector pAN7-1, a commonly used non-replicative vector for integrative transformation in fungi, yielded 5–46 resistant colonies per μg of DNA per 107 viable protoplasts. Southern blot analysis of certain transformants revealed unexpected replicative plasmids containing pAN7-1 sequences, but modified for size, methylation and restriction enzyme pattern when compared to the initial transforming vector. Two such replicative derivatives of pAN7-1 have been rescued from P. ostreatus by cloning into Escherichia coli. Rescued plasmids have been used to probe DNA from untransformed P. ostreatus in an effort to identify fungal sequences that recombined in vivo with pAN7-1 to form replicative plasmids. Such replicative sequences have been localized in high molecular weight (chromosomal) DNA of wild-type P. ostreatus. Transformation has been obtained for P. ostreatus using a rescued plasmid, thereby confirming the role of this recombinant plasmid as a shuttle vector.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0983
    Keywords: Pleurotus astreatus ; Origin of replication ; Nucleotide sequence ; Replicative transformation ; Recombinant plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Replicative plasmids generated through in-vivo recombination have been identified among transformants of the fungus Pleurotus ostreatus. In addition to sequences from a standard selection vector (pAN7-1), these recombinant plasmids contain recombined sequences of chromosomal origin conferring replicative potential upon the vector. One such recombined sequence, an 1148-bp insert into plasmid pP01, has been characterized. This sequence has been analyzed for secondary structural features as well as for consensus sites affiliated with origins of replication (ori) in other eukaryotic systems. The 1148-bp insert lacks an ORF and does not contain an acceptable match to the commonly identified 11-bp ars consensus sequence (A/TTTTATA/GTTTA/T) for autonomous replication in the yeast Saccharomyces cerevisiae. The analysis, however, revealed a cluster of three hairpin-loop-forming subsequences with individual ΔG25°C free energy values of-7.6,-6.4 and-5.2 kcal mol-1. Also found were two 7-bp analogues to centromere-affiliated sequences recognized in other fungi, as well as several putative gyrase recognition sites comparable to the 9-bp S. cerevisiae/E. coli gyrase-binding consensus sequence. Sequences comparable to the ori of the yeast 2-μm plasmid or to various sequences associated with ori of yeast/fungal mitochondrial DNAs (mtDNA) were not present in the 1148-bp insert. Replication of pP01 appears rather to involve a replication of chromosomal derivation devoid of an ars-type consensus.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Conditions suitable for the production and regeneration of Pleurotus ostreatus protoplasts from dikaryotic mycelia were examined. Three commercially available muralytic enzymes, including Sigma lysing enzyme, Novozym 234 and Novozym 234 LP, were used for production of protoplasts. Over 2 × 107 protoplasts per gram fresh weight mycelia were obtained within 1.5 h by using each of these three enzymes. The colony regeneration rate was up to 13% on potato-dextrose-agar medium containing 0.8 m mannitol. Genetic transformation was based on positive selection for resistance to hygromycin B (HmB) using the plasmid vector pAN7-1 and accomplished by either electroporation or a polyethylene glycol (PEG)-divalent cation method. P. ostreatus strains used in this study have innate sensitivity to HmB at a critical inhibitory concentration of between 40–50 μg/ml. Selection for HmB resistance of this fungus, indicative of transformation, resulted in 3–48 HmB-resistant colonies per microgram of pAN7-1 per 107 viable protoplasts. No significant differences were apparent when either transformation protocol or either P. ostreatus strain was used. The best electrical condition found for the electrotransformation of P. ostreatus is at a field strength of 2.6–2.8 kV/cm with a capacitance of 25μF and a parallel resistance of 800 ohms, corresponding to a time constant range of 10–14 ms.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0032-8332
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The following parameters of the Taiwan monkey,Macaca cyclopis, are presented and compared with other species ofMacaca: the menstrual cycle, sexual skin and vaginal desquamation changes during the menstrual cycle, time of ovulation, gestation period, breeding season, body weight of the newborn, age and body weight at menarche, body weight at first conception, spermatozoa count in the ejaculate, and the body weight at sexual maturity of the male. The many similarities in reproductive biology ofMacaca cyclopis, the rhesus monkey (Macaca mulatta), and the cynomolgus monkey (Macaca irus) include sexual skin and vaginal desquamation changes during the menstrual cycle, time of ovulation, gestation period, and placental sign. Body weight ofM. cyclopis is lower thanM. mulatta at birth, at menarche and at the first conception in females and at sexual maturation in males, is heavier thanM. irus at birth and is not different fromM. radiata at birth. No difference in menstrual cycle ofM. cyclopis was observed in animals housed in air-conditioned rooms compared to those housed in nonair-conditioned rooms. Summer amenorrhea was not observed inM. cyclopis but a high incidence of low vaginal desquamation was noted to occur in summer months. The mode of length of the menstrual cycle ofM. cyclopis is shorter thanM. mulatta and other species ofMacaca. The breeding season ofM. cyclopis in the wild extends from the end of September to January. In laboratory conditions their fertile period extends throughout the year.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Cambridge : Cambridge University Press
    The @China quarterly 47 (1971), S. 471-493 
    ISSN: 0305-7410
    Source: Cambridge Journals Digital Archives
    Topics: Linguistics and Literary Studies , History , Political Science , Sociology , Economics
    Notes: 1. After the establishment of the Chinese People's Republic in 1949, the Chinese Nationalist Government moved to the island of Taiwan and chose the city of Taipei as its new capital. In the 20 years since then, the Nationalist Government has maintained a state of “national emergency” over the whole area under its control, and administered it under martial law. It is the avowed intention of the Government to perpetuate the present situation until the day of its reconquest of the whole of China, that is, indefinitely.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0730-2312
    Keywords: angiotensin II ; G proteins ; Src tyrosine kinases ; c-Fos ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Angiotensin II stimulates a biphasic activation of Raf-1, MEK, and ERK in WB liver epithelial cells. The first peak of activity is rapid and transient and is followed by a sustained phase. Angiotensin II also causes a rapid activation of p21ras in these cells. Moreover, two Src family kinases (Fyn and Yes) were activated by angiotensin II in a time- and concentration-dependent manner. Microinjection of antibodies against Fyn and Yes blocked angiotensin II-induced DNA synthesis and c-Fos expression in WB cells, indicating an obligatory involvement of these tyrosine kinases in the activation of the ERK cascade by angiotensin II. Finally, substantial reduction of the angiotensin II-stimulated activation of Fyn, Raf-1, ERK, and expression of c-Fos by pertussis toxin pretreatment argues that G proteins of the Gi family as well as the Gq family are involved in angiotensin II-mediated mitogenic pathways in WB cells. J. Cell. Biochem. 69:63-71, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 396-402 (July 2002), p. 1715-1720 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 10
    Publication Date: 2013-06-11
    Description: The promotion of zircon ( ZrSiO 4 ) crystallization by ZnO from a zirconium-based frit glaze was studied and the possible mechanism was discussed. X-ray diffraction was used to analyze the relative quantities of zircon and other transitional crystals in the samples. The results show that ZnO can significantly decrease the crystallization temperature of zirconium-based glaze, depress the formation of Ca 2 ZrSi 4 O 12 , and promote the devitrification of transitional crystals t - ZrO 2 and Ca 2 ZnSi 2 O 7 , as well as lead to the formation of more zircon than the ZnO -free glaze. It was also found that zircon not only can form from the interaction between t - ZrO 2 and SiO 2 but also can devitrify directly from the glass phase of zirconium-based glaze.
    Print ISSN: 0002-7820
    Electronic ISSN: 1551-2916
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by Wiley
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