ISSN:
1573-0778
Keywords:
bile acids and salts
;
bile formation
;
cholestasis
;
luminescent proteins
;
organic anion transport
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Sodium-dependent uptake of bile acids from blood is aliver-specific function which is mediated by theNa+-taurocholate cotransporting polypeptide(Ntcp). We report the stable expression of aNa+-taurocholate cotransporting green fluorescentfusion protein in the human hepatoblastoma cell lineHepG2, normally lacking Ntcp expression. Ntcp-EGFPassociated green fluorescence colocalized with Ntcpimmunofluorescence in the plasma membrane. Intransfected HepG2 cells, the fusion protein mediatedthe sodium-dependent uptake of the bile acidtaurocholate (Km: 24.6 μmol/l) and of the anionicsteroids estrone-3-sulfate and dehydroepiandrosteronesulfate. We conclude that the Ntcp-EGFP fusion proteinfollows the sorting route of Ntcp, is functionallyidentical to Ntcp and could be used to monitor proteintrafficking in living HepG2 cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008152729133
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