ISSN:
1435-1536
Keywords:
Phospholipid bilayers sodium dodecyl sulphate/Triton X-100 mixed systems
;
permeability alterations and solubilization
;
carboxyfluorescein release
;
static light scattering
;
surfactant/phospholipid molar ratios and partition coefficients
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract The mechanisms governing the subsolubilizing and solubilizing interaction of sodium dodecyl sulphate (SDS)/Triton X-100 mixtures and phosphatidylcholine liposomes were investigated. Permeability alterations were detected as a change in 5(6)-carboxy-fluorescein (CF) released from the interior of vesicles and bilayer solubilization as a decrease in the static light-scattered by liposome suspensions. Three parameters were described as the effective surfactant/lipid molar ratios (Re) at which the surfactant system a) resulted in 50% of CF release (Re 50%CF); b) saturated the liposomes (Re SAT;c) led to a complete solubilization of these structures (Re SOL). From these parameters the corresponding surfactant partition coefficientsK 50%CF,K SAT andK SOL were determined. The free surfactant concentrationsS W were lower than the mixed surfactant CMCs at subsolubilizing level, whereas they remained similar to these values during saturation and solubilization of bilayers in all cases. Although theRe increased as the mole fraction of the SDS rose (X SDS), theK parameters showed a maximum atX SDS values of about 0.6, 0.4 and 0.2 forK 50%CF,K SAT andK SOL respectively. Thus, the higher the surfactant contribution in surfactant/lipid system, the lower theX SDS at which a maximum bilayer/water partitioning of mixed surfactant systems added took place and, consequently, the lower the influence of the SDS in this maximum bilayer/water partitioning.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00665642
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