ISSN:
0006-3592
Keywords:
recombinant Yarrowia lipolytica
;
heterologous protein expression
;
improvement of rice α-amylase productivity
;
cyclic fed-batch culture
;
bioprocess control strategy
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A cyclic fed-batch bioprocess is designed and a significant improvement of rice α-amylase productivity of recombinant Yarrowia lipolytica is illustrated. A bioprocess control strategy developed and reported here entails use of a genetically stable recombinant cloned for heterologous protein, use of optimized media for cell growth and enzyme production phases, and process control strategy enabling high cell-density culture and high α-amylase productivity. This process control can be achieved through maintaining a constant optimal specific cell growth rate at a predetermined value (i.e., 0.1 h-1), controlling medium feed rate commensurate with the cell growth rate, and maintaining a high cell-density culture (i.e., 60-70 g/L) for high productivity of cloned heterologous protein. The volumetric enzyme productivity (1,960 units/L · h) achieved from the cyclic fed-batch process was about 3-fold higher than that of the fed-batch culture process (630 units/L · h). © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:379-385, 1998.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
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