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  • 1
    Electronic Resource
    Electronic Resource
    The diffusion of tritiated water across isolated term human amnion (1974)
    Springer
    The journal of membrane biology 18 (1974), S. 39-48 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The diffusional permeability of isolated human amnion to tritiated water is studied in relation to its histological structure. It is shown that the high permeability of this tissue requires an evaluation of the effects on diffusion of unstirred layers of solution adjacent to the membrane. After correction for such effects the magnitude of the permeabiiity coefficient is found to be both larger and more variable than previously reported. The principal reason for this is found to lie with variations in tissue thickness. The results show that for the purposes of water diffusion the amnion may be treated as a homogeneous membrane in the axial co-ordinate of flow. This finding is discussed in relation to other evidence concerning membrane structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01870101
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  • 2
    Electronic Resource
    Electronic Resource
    Water transport across isolated term human amnion (1974)
    Springer
    The journal of membrane biology 18 (1974), S. 49-60 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The hydrodynamic permeability of normal term human amnion is measured using pressure-driven bulk flows. The permeability coefficient is found to vary widely, variations between tissues taken from different subjects being significantly greater than those from samples taken from one subject. No correlation is observed between this coefiicient and either tissue thickness or the diffusional permeability coefficient measured using tritiated water; it is, however, found to be very sensitive to epithelial damage. The results indicate that the bulk transport of water through amnion is largely controlled by the amniotic epithelium alone. This contrasts with water diffusion which is a function of total membrane thickness. The two permeability coefficients cannot therefore be employed to formulate an equivalent pore model of the whole tissue. An equivalent pore model of the epithelial layer only is considered and the results assessed in the light of other evidence bearing on the structure of amnion. It is concluded that the epithelial layer is intersected by a large number of pores with radius 10 to 30 Å, and a smaller number of much broader pores.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01870102
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  • 3
    Electronic Resource
    Electronic Resource
    Partial sequence analysis of cloned dengue virus type 2 genome
    Amsterdam : Elsevier
    Gene 46 (1986), S. 257-267 
    Details
    ISSN: 0378-1119
    Keywords: (Recombinant DNA: positive-strand RNA ; cDNA ; polyprotein segments ; yellow fever virus homology)
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(86)90410-5
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  • 4
    Electronic Resource
    Electronic Resource
    Inhibition of colonisation of the alimentary tract in young chickens with Campylobacter jejuni by pre-colonisation with strains of C. jejuni (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 182 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Strains of Campylobacter jejuni, isolated from human gastro-intestinal infection and inoculated orally into 1-day-old chicks, colonised the alimentary tract (caecum) well. There was evidence of invasion from the intestine to the spleen. Oral inoculation with some but not all strains of C. jejuni 24 h earlier (within 12 h of hatching) prevented establishment by challenge strains administered orally 1 day later. One strain which was less able to colonise the gut was less inhibitory than other strains. Precolonisation of newly hatched chicks with a strain of Salmonella typhimurium had no inhibitory effect on establishment by the challenge strain of C. jejuni and may even have exacerbated it. Inhibition of multiplication of a nalidixic acid-resistant mutant of a C. jejuni strain was prevented when it was added to a stationary-phase broth culture of the antibiotic-sensitive parent strain and the mixed culture re-incubated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb08879.x
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  • 5
    Electronic Resource
    Electronic Resource
    The association of GRB 060218 with a supernova and the evolution of the shock wave (2006)
    [s.l.] : Nature Publishing Group
    Nature 442 (2006), S. 1008-1010 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Although the link between long γ-ray bursts (GRBs) and supernovae has been established, hitherto there have been no observations of the beginning of a supernova explosion and its intimate link to a GRB. In particular, we do not know how the jet that defines a γ-ray burst emerges ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature04892
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  • 6
    Electronic Resource
    Electronic Resource
    Stratigraphic architecture resulting from Late Quaternary evolution of the Riverine Plain, south-eastern Australia (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Sedimentology 43 (1996), S. 0 
    Details
    ISSN: 1365-3091
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: The Riverine Plain of south-eastern Australia is the result of prolonged Cenozoic fluvial activity. Single thread, anabranching and distributary channels and floodplains, and associated aeolian dunes, characterize the uppermost sequences. Based on detailed interpretations of Late Quaternary fluvial sedimentation and surficial stratigraphy for this 77 000-km2 basin, earlier ‘prior stream’ and ‘ancestral stream’ models of fluvial deposition, deduced from limited stratigraphic and chronological evidence, are replaced with aggradational palaeochannel and migrational palaeochannel models. Thermoluminescence dating reveals four distinct phases of palaeochannel activity between 105 and 12 ka; the first (Coleambally phase) late in Oxygen Isotope Stage 5, the second (Kerarbury phase) in Stage 3, the third (Gum Creek phase) before and the fourth (Yanco phase) after the Last Glacial Maximum (LGM) in Stage 2. The first three of these phases were characterized by mixed-load laterally migrating sinuous palaeochannels with occasional transitions to a straighter bedload-dominated mode, and vice versa. The first two phases concluded with a bedload-dominated episode resulting in aggradational palaeochannels on the surface of the Plain, and the third phase (prior to the LGM) did also in its downstream reaches. The phase following the LGM was characterized entirely by large mixed-load sinuous migrational palaeochannels. These exhibited no terminating bedload episode, because the onset of Holocene climates reduced the size of the flood peaks, greatly diminished the supply of bedload from the upper catchments and resulted in streams evolving to their present highly sinuous suspended load form. The result is a complex stratigraphic architecture consisting of vertically and laterally accreted units extending over hundreds of kilometres in the form of channel-sand stringers, sand sheets and derivative aeolian dunes partially or wholly encased in overbank fines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3091.1996.tb01512.x
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  • 7
    Electronic Resource
    Electronic Resource
    The integral membrane protein from a virulent isolate of transmissible gastroenteritis virus: molecular characterization, sequence and expression in Escherichia coli (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 2 (1988), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Subgenomic mRNA from a virulent isolate of porcine transmissible gastroenteritis virus (TGEV) was used to produce cDNA clones. Part of a new clone and a previously reported clone were sequenced and used to construct the viral gene for integral membrane protein. A single open reading frame (ORF) encoding a polypeptide of 262 amino acids, relative molecular mass (Mr) 29459, was identified. The positive identification of the polypeptide as the integral membrane protein was demonstrated by the production in E. coli of a chimaeric protein comprising most of the ORF encoding the Mr 29459 polypeptide and β-galactosidase. The chimaeric protein reacted with a specific monoclonal antibody to viral integral membrane protein and antibodies raised against the chimaeric protein immune precipitated the viral protein. Comparison with the sequence of an avirulent isolate indicates amino acid residues that may be important in pathogenicity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1988.tb00056.x
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  • 8
    Electronic Resource
    Electronic Resource
    Sequence of the nucleoprotein gene from a virulent British field isolate of transmissible gastroenteritis virus and its expression in Saccharomyces cerevisiae (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 2 (1988), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Subgenomic mRNA from a virulent isolate of porcine transmissible gastroenteritis virus (TGEV) was used to produce cDNA which was sequenced. Two non-overlapping open reading frames (ORFs) were identified. The largest, encoding a polypeptide of 382 amino acids (relative molecular mass (Mr) 43 483), was shown to be the viral nucleoprotein gene. The second ORF, found 3’to the larger ORF, encodes a polypeptide of 78 amino acids (Mr 9068) which has yet to be assigned to a viral product. The nucleoprotein gene was expressed in yeast cells under the control of two types of yeast promoters: the constitutive PGK promoter, and the inducible GAL1 promoter. Yeast cells containing recombinant plasmids, with the nucleoprotein gene in the correct orientation, produced a polypeptide of M, 47000, identical to the viral product, that reacted with a specific monoclonal antibody.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1988.tb00010.x
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  • 9
    Electronic Resource
    Electronic Resource
    The Teorell membrane oscillator as a mechano-electric transducer (1973)
    Springer
    The journal of membrane biology 11 (1973), S. 197-216 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The results of a recent quantitative analysis of the Teorell membrane oscillator are utilized to explore its role as an excitability analogue. Special attention is paid to its role as a mechano-electric transducer. A membrane of exceptionally well-defined pore structure has been used in this study. The analogue properties arise from nonlinear coupling between water and salt fluxes. When the membrane is simultaneously subjected to controlled gradients of hydrostatic pressure, electrical potential and concentration, bi-stable stationary states can be produced. These arise from the opposing effects of pressure and electro-osmosis on the volume flow. Transitions between these states show hysteresis. The factors governing such transitions are analogous to certain types of stimuli encountered in the natural excitation process. The membrane system also shows oscillatory behavior when the hydrostatic pressure gradient is allowed to vary under constant current conditions. This property is related to the bi-stable stationary state phenomena and is compared to the regenerative behavior found in biologically excitable tissues. Particular emphasis is placed upon analogies between the membrane oscillator and certain natural tissues. The importance of the nonlinear nature of the force-flux coupling in the analogue is stressed, and its possible relevance to biological excitability indicated. Some consideration is also given to the role of electro-osmotic flux coupling in biological tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01869822
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  • 10
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    A functional role for the two-pore domain potassium channel TASK-1 in cerebellar granule neurons (2000)
    National Academy of Sciences
    Details
    Publication Date: 2000-03-28
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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