Application of Aquifer Thermal Energy Storage with High Temperatures (HT-ATES) ranging from 60–90 is a promising technique to store large amounts of energy in urban areas. However, these areas typically lack information on hydrogeological and thermal parameters of the subsurface to determine the potential for energy storage. Moreover, conventional exploration methods as pumping tests do not account for the variation in density caused by the high temperature gradients or changes in salinity as encountered in HT-ATES operation. The objective of this study is therefore to develop best practices for characterizing the hydrogeological and thermal properties of groundwater wells and their surrounding formation that determine the potential performance of HT-ATES-systems. In addition to conventional pumping tests, a set of Push–Pull tracer Tests (PPTs) with cold and hot water are proposed and scrutinized using Berlin as case study. There, the research well Gt BChb 1/2015, which is characterized by a reservoir temperature of 17 at a depth between 220 und 230 m below ground surface was tested. In 2017, seven Slug-Withdrawal Tests (SWTs), a Step-Rate-Test (SRT), a production tests, and two Push–Pull tracer Tests (PPTs) with hot and cold water were performed during a period of 40 days. These tests were accompanied by Distributed-Temperature-Sensing (DTS) monitoring. The temperature measurements provide indications of injection areas based on the warmback period during a PPT with 81 hot water. The determined aquifer transmissibility , the related Productivity Index (), and maximum flow rates of about indicate that the aquifer has potential for HT-ATES. However, the PPT and the DTS monitoring revealed cross flow between the target aquifer and an overlying aquifer. Thus, a new well with a design avoiding cross flow is required to utilize the aquifer’s energy storage potential. A set of best practices for characterizing HT-ATES potential was derived from the experiences in this study.