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C1q production and secretion by fibroblasts (1976)

AL-ADNANI, M. S. ; O'D McGEE, J.

[s.l.] : Nature Publishing Group

Nature 263 (1976), S. 145-146

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Three fibre-blast lines were used: human lung fibroblasts (from 13-weekembryos) obtained from Gibco-Biocult (Paisley, Scotland); human skin fibroblasts (from 14?16-week embryos) and rat skin fibroblasts (from 16?18-day embryos) cultured in this laboratory. All cell lines were grown in air on glass ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/263145a0

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Discrimination of closely homologous human genomic and viral sequences in cells and tissues: further characterization of Tmt (1994)

Herrington, C. S. ; O'D. Mcgee, J.

Springer

Journal of molecular histology 26 (1994), S. 545-552

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ISSN: 1573-6865

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary The experimentally derived parameter Tmt (tissue Tm) was defined previously to describe the end-point used for evaluation of the stringency of non-isotopic in situ hybridization and was found to differ from the theoretical melting temperature (Tm) for several HPV types. In this paper, the reasons for this discrepancy were investigated by performing a series of experiments with a variety of probes for both human genomic and integrated viral sequences in isolated and cultured normal and abnormal cells in addition to paraffin-embedded material. Tmt was shown to be dependent on several parameters of probe and target, and on the sensitivity of the detection system used but was not affected by aldehyde fixation or paraffin wax embedding under optimal conditions of nucleic acid unmasking. These data support the hypothesis that differences between Tmt and Tm may be due to the use of a different end-point for in situ hybridization analysis rather than biochemical alteration of DNA-DNA interactions in intact cells. Appropriate stringency conditions should therefore be determined by experiment rather than calculated theoretically for gene evaluation in cells and tissues.