ISSN:
1573-3904
Keywords:
Protein de novo design
;
Template-Assembled Synthetic Proteins (TASP)
;
Oxime bond formation
;
Orthogonal protection
;
Peptide synthesis
;
Helix bundle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The use of chemoselective ligation methods and orthogonal protection techniques allows access to Template-Assembled Synthetic Protein (TASP) molecules exhibiting a large variety of packing topologies. This is demonstrated for the synthesis of an antiparallel 4α-helical bundle TASP by condensing amphiphilic peptide blocks, containing aldehyde functions at the C- or N-terminus, to a selectively addressable topological template via oxime bond formation. The resulting antiparallel 4α-helix TASP is obtained in high yield and shows a template-induced helical conformation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00132757
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