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  • 1
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    Methods for the quantification ofFrankia cell biomass (1989)
    Springer
    Details
    Publication Date: 1989-08-01
    Print ISSN: 0032-079X
    Electronic ISSN: 1573-5036
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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  • 2
    Electronic Resource
    Electronic Resource
    End-probing: A non-radioactive approach to mapping transposon insertions (1998)
    Springer
    Methods in cell science 20 (1998), S. 113-118 
    Details
    ISSN: 1573-0603
    Keywords: End-labeling ; GBS ; Mapping ; Restriction enzyme mapping ; Tn917
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two pieces of data are needed to fully map the location of a transposon inserted in a plasmid, the site of insertion and the transposon's orientation. Both of these parameters can be determined from a map of restriction sites, which can be derived by end-probing. Like restriction mapping, end-probing reveals the distance between restriction sites on a plasmid. In contrast to restriction mapping, end-probing unambiguously reveals the order of those restriction sites. End-probing is similar to end-labeling, except that the uncertainties inherent in the radiolabeling reaction (and the problems of working with radionucleotides) are avoided. In this paper we discuss the use of end-probing as a means to map insertions of transposon Tn917 into the Streptococcus agalactiae plasmid pGB354
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009851211362
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Methods for the quantification ofFrankia cell biomass (1989)
    Springer
    Plant and soil 118 (1989), S. 199-204 
    Details
    ISSN: 1573-5036
    Keywords: biomass ; Frankia ; methods ; packed cell volume ; protein ; quantification ; turbidity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Six methods for the estimation of microbial biomass were compared for determination ofFrankia cell concentrations. Six strains ofFrankia were cultivated in stationary culture, harvested by centrifugation, washed with saline buffer and diluted to five standardized concentrations. These cell suspensions were then used to assess reliability of each of the biomass determination methods. The destructive total protein determination methods were the most sensitive and reliable. Two non-destructive methods, packed cell volume and turbidity measurement, were also accurate, and because of their simplicity hold advantage for routine growth measurements and inoculum dilutions. Dry weight determinations were inconsistent for the small cell masses used in this study. An ELISA procedure demonstrated reliability but little sensitivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02232807
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    Paper (German National Licenses)
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