Publication Date:
2017-01-26
Description:
Nature Materials 16, 230 (2017). doi:10.1038/nmat4772 Authors: David R. Myers, Yongzhi Qiu, Meredith E. Fay, Michael Tennenbaum, Daniel Chester, Jonas Cuadrado, Yumiko Sakurai, Jong Baek, Reginald Tran, Jordan C. Ciciliano, Byungwook Ahn, Robert G. Mannino, Silvia T. Bunting, Carolyn Bennett, Michael Briones, Alberto Fernandez-Nieves, Michael L. Smith, Ashley C. Brown, Todd Sulchek & Wilbur A. Lam Haemostasis occurs at sites of vascular injury, where flowing blood forms a clot, a dynamic and heterogeneous fibrin-based biomaterial. Paramount in the clot’s capability to stem haemorrhage are its changing mechanical properties, the major drivers of which are the contractile forces exerted by platelets against the fibrin scaffold. However, how platelets transduce microenvironmental cues to mediate contraction and alter clot mechanics is unknown. This is clinically relevant, as overly softened and stiffened clots are associated with bleeding and thrombotic disorders. Here, we report a high-throughput hydrogel-based platelet-contraction cytometer that quantifies single-platelet contraction forces in different clot microenvironments. We also show that platelets, via the Rho/ROCK pathway, synergistically couple mechanical and biochemical inputs to mediate contraction. Moreover, highly contractile platelet subpopulations present in healthy controls are conspicuously absent in a subset of patients with undiagnosed bleeding disorders, and therefore may function as a clinical diagnostic biophysical biomarker.
Print ISSN:
1476-1122
Electronic ISSN:
1476-4660
Topics:
Chemistry and Pharmacology
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Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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Natural Sciences in General
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Physics
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