ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1
    Publication Date: 2018-10-05
    Description: In order to perform the project, 446 samples of rainbow trout (Onchorhynchus mykiss) from 24 different regions in Iran were collected. About 2-3 g of caudal fin samples was collected from each specimen and preserved in absolute ethyl alcohol and then transferred to the genetic laboratory. Genomic DNA was extracted using the phenol-chloroform method and then DNA content and quality was determined using spectrophotometry and agarose gel electrophoresis, respectively. Polymerase Chain Reaction (PCR) of genomic DNA fin samples was carried out using 10 pairs of microsatellite primers. All PCR products were electrophoresed on 6% polyacrylamide gel and stained with silver nitrate. Following the scoring of alleles, all parameters including allelic frequency, effective number of allele, observed and expected heterozygosity, shanon index, measurement of similarity and genetic distance and Hardy-Weinberg equilibrium, Fst , Rst and gene flow were calculated using AMOVA analysis in the GenAlex and Popgene programs. The results showed that 8 pairs of microsatellite primers were polymorphic. In total, 50 alleles were determined with the range size of 64-280 bp. The locus omyf had maximum number of allele (26) and loci OTSG 474 and Strurruta58 had minimum number of allele (5). The observed heterozygosity was between 0.86 and 0.964. Hardy-Weinberg departure was observed for all loci from farms 18, 15, 4, E20 and 21 and were disequilibrium (P〈0.05). The farms 14, 8, 7 and 6 were equilibrium at 3 loci, but showed disequilibrium in other loci. The other farms were equilibrium at 1 or 2 loci and disequilibrium at 8 or 9 loci. The FST results showed that maximum FST (0.24) were between farms 1 and 11in which had minimum of gene flow (3.7). Minimum FST (0.04) were between farms 8 and 9 in which had maximum of gene flow (346). Based on the results of AMOVA analysis, significant differences were detected between all farms (P〈0.01). Furthermore, based on Nei 's standard (1972) maximum genetic distance (0.89) were observed between farms 2 and 11 and maximum genetic similarity (0.15) were detected between farms 3 and 4. This result suggests that the unique genetic variation of rainbow trout in hatchery farms of Iran represents a highly valuable genetic resource and provide useful information for creating a based population in the future breeding programs.
    Keywords: Biology
    Repository Name: Aquatic Commons
    Type: Monograph or Serial Issue , NonPeerReviewed
    Format: application/pdf
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Publication Date: 2018-09-14
    Description: This project in partnership with Azerbaijan State Fisheries Department and the aim of increasing fish production hydrothermal (Carp fish) at unit area through providing scientific and practical method in applying air device and nutrition management, a field area of 8.5 hectares and in ponds with 3300 m^2 at village Eylarzgy carried at Urmia city. After preparation of ponds, action to release fry density was 6000 ha piece. Percent including 55 percent of silver carp (as most fish), Common carp 31 percent, 8 percent Grass carp and 6 percent of Big head with average weights 67 to 92 grams respectively. For fertilizing ponds in addition to basic fertilizer, during development, organic fertilizers (cow) amount to 24 ton/ hectar (Aslary) once every two days and types of mineral fertilizers (chemical) rate of 1012.5 kg per in three days once were used. In addition to feeding carp fish with natural food, concentrates and food for feeing Grass carp fresh forage (alfalfa) was used. Oxygen supply required for ponds were provided with one to two air units (Air jet). Sampling and biometry of fish and water samples for water analysis and identification of plant zooplankton (of the family and genus) once every month until the end of the growing period was performed. Resulting data were analyzed with Excel statistical software. Results showed that the production rate in the studied ponds was 4.74 tons per hectare and production of 3.15 tons per hectare in year ago, the farm has 50.5 percent increase compared to year ago average production rate in the whole province (3.21 tons per hectare) of 47.7 percent and the average production rate in the country in 1385 (about 3.8 tons per hectare) 24.7 percent increase shows.
    Keywords: Aquaculture
    Repository Name: Aquatic Commons
    Type: Monograph or Serial Issue , NonPeerReviewed
    Format: application/pdf
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    Publication Date: 2018-10-05
    Description: Breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.
    Keywords: Aquaculture
    Repository Name: Aquatic Commons
    Type: Monograph or Serial Issue , NonPeerReviewed
    Format: application/pdf
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    Publication Date: 2018-10-13
    Description: In order to study plankton population composition and species diversity in different regions of Aras Rivers and the evident reservoir sampling was performed monthly from determined sampling sites from April 2007 to March 2008. Sampling was conducted in Reservoir Lake by a PVC pipe with 2.25 m length and 5 cm diameter by columnar method. Besides, in more dipper (〉5 m) sites, sampling conducted from bottom to surface with 1-m intervals (layer sampling) by a 2-liter Ruttner type sampler. In river due to water flow sampling was conducted by a scaled measure. Phytoplankton sampling was performed by 1-liter bottles. Zooplankton was sampled by filtering of 30 L through 55- micron plankton net. These samples were then transferred into bottles and sampling date and the name of station were attached on the bottles. Then, the samples were fixed immediately and transported to laboratory. It should be mentioned some physical parameters were determined in situ. Also, a 1liter and another 2-liter water were collected to determine chlorophyll a and chemical factors respectively. Samples were preserved in dark and cold place for a week. The upper layers (without phytoplankton) were removed and the lower layer (with phytoplankton) was sampled into 5-ml chambers. The enumeration and identification was performed by a Nikon TS100 inverted microscope with ×400 (Phytoplankton) and ×200 (Zooplankton) magnification according to Utermohl (1958) method. About 50 fields were enumerated for each sample. The comparison of physicochemical and biological factors with standard criterion indicated that the presence of a fertile plain, agricultural growth, drought and higher water demand in recent years, as well as, higher population density, the Nakhjavan city location and sewage water flows into the Aras reservoir, the lake is going to be an eutroph lake. Sampling of crayfish was performed from 5 sampling sites seasonally. Aras reservoir freshwater crayfish biomass was sampled in 1391, by putting 100 funnel-shaped nets. At each site one row nets composed of 20 nets were put by rocks, connecting robe and boat. Twenty four hours later, total freshwater cray fish was sampled (at least 300 crayfish was randomly sampled from each site). The samples were transported to laboratory and their sex, sex percentage, biometric characteristics including length, weight were recorded. Length and weight groups were estimated totally and for males and females in each season. The average total length and weight were 106.43 ± 7.94 mm and 35.81 ± 10.86 gr respectively which both are lower than West-Azarbaijan fisheries criterion. In this study only 18.99 percent contained a length higher than exporting level (120 mm). Yet, only 16.46 percent of harvesting had higher than 50 g (exporting level). With regard to data, freshwater shrimp (Astacus leptodactylus) in Aras dam reservoir stock has reduced. In general, male-female ratio was high. The comparison of growth equations confirms that males had higher weights than equal females. In recent years, the freshwater crayfish harvesting has been declined due to increased harvesting and over loading of nutrients into the lake.
    Keywords: Biology ; Environment
    Repository Name: Aquatic Commons
    Type: Monograph or Serial Issue , NonPeerReviewed
    Format: application/pdf
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...