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  • 1
    Publication Date: 2021-05-19
    Description: Halophiles are in all three domains of life: Archaea, Bacteria and Eucarya. Halophilic microorganisms in addition to form major part of life biodiversity can have many biotechnological applications. The objective of this research is isolation and identification of halophilic bacteria from Urmia Lake in Iran and the study of its bacterial biodiversity. After sampling of brines from Urmia Lake from 10 stations and depth of approximately 30-50 cm, in April 2011 and transfer to the laboratory in the sterile conditions, samples were enriched and cultured on defined media, and incubated. After appearance of colonies, selected strains were studied based on morphology, physiology and biochemical characteristics. For phylogenetic identification, their genomic DNA were extracted and amplified by PCR technique. Therefore their sequences were determined by genetic experiment based on 16S rRNA gene sequence and their similarity were analysed in GenBank of EzTaxon database. Finally the phylogenetic tree was constructed. Studied strains belonged to three genera: Halomonas 50% (including H. andesensis LC6(T) [12.5%], H. gomseomensis M12(T) [12.5%], H. hydrothermalis Slthf2(T) [12.5%], H. boliviensis LC1(T) [6.25%] and H. janggokensis M24(T) [6.25%]), Salinivibrio 25% (including S.costicolasubsp. alcaliphilus DSM 16359(T) [18.75%] and S. sharmensis BAG(T) [6.25%]) and Idiomarina 25% (including I .loihiensis L2TR(T) [25%]).
    Description: Published
    Keywords: Ecology ; Halophilic bacteria ; Species diversity ; Isolation ; Phylogenetic ; DNA analysis
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.45-59
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  • 2
    Publication Date: 2021-05-19
    Description: This study aimed to investigate the toxic effects of atrazine herbicide on the fry of Caspian Kutum (Rutilus frisii kutum, Kamensky, 1901). First the 96-h LC50 of the fry were exposed to atrazine at the concentration of 24.95 ppm was determined. Then the toxicity of this herbicide on Caspian kutum fry exposed to the concentration of 12.47ppm (1/2 LC50), for four days was measured and compared with a control group. Comparison of the length, weight and condition factor showed no significant differences between atrazine exposed and control group. The concentration of Na+, K+, Ca2+, Mg2+ and Cl- in the whole body of fry in control and atrazine exposure groups were as the following order: Ca2+〉K+〉 Na +〉 Cl- 〉Mg2+ and Ca2+〉Na+〉K+〉Mg2+〉Cl-, respectively. Results showed that the concentration of all these ions were higher in atrazine exposure group than control group, except for Cl-, and the only significant differences was found in Na+ concentration. Major histopathological effects of atrazine on the gills were hyperplasia and thickening of the filaments, separation of the pavement cells of the lamellae epithelium from the pillar cells and swelling of the epithelial cells. Results of the present study showed that atrazine could affect the ion composition of the body, and caused major damages in gill epithelium even at sublethal concentration and acute exposure, but had no effects on the growth parameters.
    Description: Published
    Keywords: Biology ; Physiology ; Caspian Kutum ; Atrazine ; Rutilus frisii kutum ; Toxicity ; Ion ; LC50 ; Histopathological ; Growth ; Parameters
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.702-718
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  • 3
    Publication Date: 2021-05-19
    Description: Considering the ever increasing population and industrialization leading to developmental trend of humankind's life, we are hardly able to detect the toxins produced in food products using traditional techniques. In this technique, the production of molecular framework and polymer is done using meta acrylic acid monomers, which are formed via covalence connection between meta acrylic acid monomers (MAA) of white polymer. Here also hydrogenised connection between exotoxin amino acid and meta acrylic acid is made that would function as the selective absorption for that. Then in the second stage, based on the bacterial antibody connection to nanoparticle, a sensor was used. In this part of the research, as the basis for absorption for the recognition of bacterial toxin, medium sized silica nanoparticles of 10 nanometer in the form of solid powder were utilized with Notrino brand. Then the suspension produced from agent-linked nanosilica which was connected to bacterial antibody was positioned near the samples of distilled water, that were contaminated with Staphylococcus aureus bacterial toxin with the density of 10-3, so that in case any toxin exists in the sample, a connection between toxin antigen and antibody would be formed. Finally, the light absorption related to the connection of antigen to the particle attached antibody was measured using spectrophotometers. The results indicate that the molecular framework polymer sensor is capable of detecting up to the density of 10-3, but not lower than that, whereas using the second sensor, up to 10-4 of density is detectable. Additionally, the sensitivity of the sensors were examined after 60 days and the first sensor by the day of 28 and the second sensor by the 56 day had confirmatory results and started to decrease after those time periods.
    Description: Published
    Keywords: Biology ; Physiology ; Nanobiosensor ; Staphylococcus aureus ; Exotoxin ; Molecular Framework Polymer Nanosensor
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.667-683
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  • 4
    Publication Date: 2021-05-19
    Description: This study was aimed to investigate the effect of vacuum packing (VP) on the fatty acids profiles in cobia (Rachycentron canadum) fillets during an extended frozen storage period. Cobia fillets were treated under vacuum system then stored at -18°C for up to 6 months and compared to control conditions. As a result of a frozen storage period of 6 months, a marked content decrease was found in the fatty acid groups such as MUFA, PUFA and ω -3 PUFA, as well as in the ω -3/ ω-6 ratio. However, a preserving effect on such fatty acid parameters could be observed due to the VP treatment. Assessment of the polyene index (PI) indicated an increased lipid oxidation development as a result of the frozen storage time however, this increase was partially inhibited by the vacuum packaging. Results indicate that vacuum packaging was a proper way to reduce lipid oxidation in Cobia fillets and extend their shelf life by omitting available oxygen. Thus the employment of VP alone or in combination with other protective strategies is recommended.
    Description: Published
    Keywords: Fish processing ; Rachycentron canadum ; fillets ; Packaging ; Fatty acids ; Vacuum packaging ; Frozen storage ; Cobia Fish
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.275-288
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  • 5
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    In:  http://aquaticcommons.org/id/eprint/22719 | 18721 | 2018-05-17 18:34:08 | 22719 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-09
    Description: This study aimed to investigate the toxic effects of atrazine herbicide on the fry of Caspian kutum (Rutilus frisii kutum, Kamensky, 1901). First the 96-h LC50 of the fry were exposed to atrazine at the concentration of 24.95 ppm was determined. Then the toxicity of this herbicide on Caspian kutum fry exposed to the concentration of 12.47ppm (1/2 LC50), for four days was measured and compared with a control group. Comparison of the length, weight and condition factor showed no significant differences between atrazine exposed and control group. The concentration of Na+, K+, Ca2+, Mg2+ and Cl- in the whole body of fry in control and atrazine exposure groups were as the following order: Ca2+〉K+〉Na+〉Cl-〉Mg2+ and Ca2+〉Na+〉K+〉Mg2+〉Cl-, respectively. Results showed that the concentration of all these ions were higher in atrazine exposure group than control group, except for Cl-, and the only significant differences was found in Na+ concentration. Major histopathological effects of atrazine on the gills were hyperplasia and thickening of the filaments, separation of the pavement cells of the lamellae epithelium from the pillar cells and swelling of the epithelial cells. Results of the present study showed that atrazine could affect the ion composition of the body, and caused major damages in gill epithelium even at sublethal concentration and acute exposure, but had no effects on the growth parameters.
    Keywords: Biology ; Fisheries ; Atrazine ; Rutilus frisii kutum ; Toxicity ; Ion ; LC50 ; Biology ; Physiology ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 702-718
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  • 6
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    In:  http://aquaticcommons.org/id/eprint/22717 | 18721 | 2018-05-17 18:27:23 | 22717 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-09
    Description: Considering the ever increasing population and industrialization leading to developmental trend of humankind's life, we are hardly able to detect the toxins produced in food products using traditional techniques. In this technique, the production of molecular framework and polymer is done using meta acrylic acid monomers, which are formed via covalence connection between meta acrylic acid monomers (MAA) of white polymer. Here also hydrogenised connection between exotoxin amino acid and meta acrylic acid is made that would function as the selective absorption for that. Then in the second stage, based on the bacterial antibody connection to nanoparticle, a sensor was used. In this part of the research, as the basis for absorption for the recognition of bacterial toxin, medium sized silica nanoparticles of 10 nanometer in the form of solid powder were utilized with Notrino brand. Then the suspension produced from agent-linked nanosilica which was connected to bacterial antibody was positioned near the samples of distilled water, that were contaminated with Staphylococcus aureus bacterial toxin with the density of 10^-3, so that in case any toxin exists in the sample, a connection between toxin antigen and antibody would be formed. Finally, the light absorption related to the connection of antigen to the particle attached antibody was measured using spectrophotometers. The results indicate that the molecular framework polymer sensor is capable of detecting up to the density of 10^-3, but not lower than that, whereas using the second sensor, up to 10^-4 of density is detectable. Additionally, the sensitivity of the sensors were examined after 60 days and the first sensor by the day of 28 and the second sensor by the 56 day had confirmatory results and started to decrease after those time periods.
    Keywords: Biology ; Fisheries ; Nanobiosensor ; Staphylococcus aureus ; Exotoxin ; Molecular Framework Polymer Nanosensor ; Biology ; Physiology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 667-683
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  • 7
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    Islamic Azad University, Science and Research Branch, Tehran, Aquatic Animal Health
    In:  http://aquaticcommons.org/id/eprint/20487 | 17408 | 2016-07-18 01:19:58 | 20487 | Islamic Azad University, Science and Research Branch, Tehran
    Publication Date: 2021-07-10
    Description: Economical achievement of optimal growth in developing countries may lead to sustainable poverty reduction. Agricultural activities play an important role in economy and human being welfare, which leads to establishment of food security and quality. Aquaculture products in developing countries share 51.4 percent of total agricultural production and 241 percent in developed countries. Therefore undoubtedly food production by means of quality and quantity has to be increased .The history of shrimp production goes back to 500 years ago. Today 50 countries of the world produce shrimp. In Islamic Republic of Iran shrimp production started since 1992 in the coastal region of Persian Gulf. The shrimp culture farms can be classified in to 4 different categories; extensive, semi-extensive, intensive and super intensive. Global ecological maintenance is one of the major concerns of authorities Human manipulation of nature is the most destructive activity. Industrial sewage leakage in to the rivers and water sources is a big issue that causes reduction in the aquatic population. Heavy metals have an inhibitory effect in the production and growth of sea life. Human intake of food treated with anti-microbial cause's allergy, hypersensitivity and develops microbial resistance. Organochlorine compounds contamination may found in hepatopancreatic tissue of aquatic products, Arsenic may transfer to man via plant and animal product contamination.
    Description: PhD
    Description: Advisors: Razavilar, V.; Counsellors: Shojaei, F., Tabatabae, A.
    Keywords: Aquaculture ; Environment ; Fisheries ; Pollution
    Repository Name: AquaDocs
    Type: thesis
    Format: application/pdf
    Format: application/pdf
    Format: 168
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  • 8
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    In:  http://aquaticcommons.org/id/eprint/22715 | 18721 | 2018-05-16 21:33:25 | 22715 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-09
    Description: Viral pathogens are major causes of outbreaks in shrimp farms throughout the world. Monodon baculovirus has been known to be invasive in 85-100% of the shrimp hatcheries, in early or late stages of shrimp. Three-hundred and sixty juvenile of Litopenaeus vannamei with average (±SD) size of 7.99±0.54 g and 3600 post larvae 10-15 were prepared from Shrimp Research Station located in Helleh and 3 hatcheries from Bushehr Province, southern part of Iran, respectively. They were allocated to 9 glass aquariums (50×50×60cm) as 2 groups (postlarvae and juvenile), controls with 3 replications using 100 liters well aerated water. They were exposed to MBV as water-born path. Concerning the pathogenesis, three phases were observed. The quantity of intranuclear occlusion bodies was 1-9. Mortality was only observed in postlarvae but not in adult or control groups. It is concluded that MBV can be an invasive pathogen for SPF offspring or postlarvae of L. vannamei being imported to Iran.
    Keywords: Aquaculture ; Biology ; Fisheries ; MBV ; Ultrastructure ; TEM ; Litopenaeus vannamei ; Biology ; Physiology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 640-652
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  • 9
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    In:  http://aquaticcommons.org/id/eprint/22730 | 18721 | 2018-05-17 23:49:11 | 22730 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-09
    Description: Sponges, which constitute the phylum Porifera, are the most primitive of the multicellular animals, among all marine organisms screened. Marine sponges produce the largest number of structurally diversified natural products. In this study we investigated in vitro antimicrobial activity of Ircinia mutans collected from the Kish Island in the Persian Gulf against strains of bacteria Escherichia coli (ATCC 15224), Pseudomonas aeruginosa (ATCC 25619), Staphylococcus aureus (ATCC 1764), and Bacillus subtilis spizizenii (ATCC 6633). Diethyl etter, methanol and aqueous extracts of sponge were evaluated by using the Bacterial Broth Dilution Method. The results showed that the aqueous extracts didn’t have any antibacterial activity. Minimum Inhibitory Concentrations (MIC) of the winter diethyl etter extract was 2 mg/ml for E.coli and 20 mg/ml for P. aeruginosa, whereas the summer diethyl etter extract and both of methanol extracts did not show any activity. The MIC and MBC (Minimum Bacterial Concentration) of summer diethyl etter extracts were 2 mg/ml and 3mg/ml against S. aureus; and 5mg/ml and 10mg/ml when tested on B. subtilis. The MIC and MBC of winter diethyl etter extracts were measured as 1.5 mg/ml and 2mg/ml against S. aurous; and 5mg/ml and 10mg/ml when examined on B. subtilis. Summer and winter methanol and aqueous extracts of I. mutans did not show any activity against these bacteria. Therefore secondary metabolite solutions in diethyl etter contain components with antibacterial properties and can be used as antibiotics products.
    Keywords: Biology ; Fisheries ; Sponge ; Secondary metabolites ; Antibacterial activity ; Kish Island ; Persian Gulf ; Biology ; Physiology ; Iran ; Pseudomonas aeruginosa ; Escherichia coli ; Bacillus subtilis spizizenii
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 823-833
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  • 10
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    In:  http://aquaticcommons.org/id/eprint/22755 | 18721 | 2018-05-21 17:48:58 | 22755 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-09
    Description: Halophiles are in all three domains of life: Archaea, Bacteria and Eucarya. Halophilic microorganisms in addition to forming a major part of life biodiversity can have many biotechnological applications. The objective of this research is isolation and identification of halophilic bacteria from Urmia Lake in Iran and the study of its bacterial biodiversity. After sampling of brines from Urmia Lake from 10 stations and depth of approximately 30-50 cm, in April 2011 and transfer to the laboratory in the sterile conditions, samples were enriched and cultured on defined media, and incubated. After appearance of colonies, selected strains were studied based on morphology, physiology and biochemical characteristics. For phylogenetic identification, their genomic DNA were extracted and amplified by PCR technique. Therefore their sequences were determined by genetic experiment based on 16S rRNA gene sequence and their similarity were analysed in GenBank of EzTaxon database. Finally the phylogenetic tree was constructed. Studied strains belonged to three genera: Halomonas 50% (including H. andesensis LC6(T) [12.5%], H. gomseomensis M12(T) [12.5%], H. hydrothermalis Slthf2(T) [12.5%], H. boliviensis LC1(T) [6.25%] and H. janggokensis M24(T) [6.25%]), Salinivibrio 25% (including S. costicola subsp. alcaliphilus DSM 16359(T) [18.75%] and S. sharmensis BAG(T) [6.25%]) and Idiomarina 25% (including I. loihiensis L2TR(T) [25%]).
    Keywords: Biology ; Limnology ; Halophilic bacteria ; Species diversity ; Isolation ; Phylogenetic ; DNA analysis ; Urmia Lake ; Ecology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 45-59
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