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  • 1
    ISSN: 1420-9098
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Die Vererbung eines Gruppenmerkmales, der Alarmierungsreaktion von Arbeiterinnen (Apis mellifera L.) wurde in einem quantitativen Stoffwechseltest überprüft. In einer diallelen Testkreuzung von selektierten Königinnen und Drohnen konnten genetische Varianzkomponenten sowie maternale Effekte geschätzt werden. Experimente, in denen Eier von fremden Pflegevölkern zu Imagines aufgezogen wurden, zeigten, dass die Stockumwelt während der Larval-und Pupalentwicklung keinen Einfluss auf das spätere Alarmverhalten der Arbeiterinnen nimmt.
    Notes: Summary The inheritance of a group character, the alarm behaviour of honey bee workers (Apis mellifera L.), was analyzed using a metabolic bio-assay. In a diallel test cross of preselected queens and drones, genetic variance and maternal effects on this behaviour were estimated. Crossfostering experiments showed that the hive environment during larval and pupal development has only minor effects on alarm behaviour.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Insectes sociaux 33 (1986), S. 26-31 
    ISSN: 1420-9098
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Einzelne Arbeiterinnen (Apis mellifera L.) bekannten Alters wurden mit einer gefärbten Zuckerwasserlösung gefüttert (Spenderbiene). In einem Alter von 5–8 Tagen und nach dem 30. Tag fütterten Spenderbienen signifikant weniger Zuckerwasser an eine Gruppe von 30 Empfängerbienen weiter als Bienen anderer Altersstufen. Dies zeigt sich sowohl bei der Anzahl gefütterter Empfängerbienen als auch bei der Futtermenge, die an jede einzelne gefütterte Biene weitergegeben wurde.
    Notes: Summary Individual worker bees (Apis mellifera L.) of known age (donor bees) were artificially fed with sugar syrup containing Methylene blue as a tracer dye. The donor bee was placed into a group of 30 recipient workers and the amount of transferred food was quantified. Donor bees, at the age of 5–8 days and older than 30 days, fed significantly less sugar syrup to recipient workers than bees of other ages. This was found for both the number of fed recipients, and for the amount of food received per recipient bee.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Insectes sociaux 32 (1985), S. 104-105 
    ISSN: 1420-9098
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Insectes sociaux 37 (1990), S. 90-99 
    ISSN: 1420-9098
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Arbeiterinnengruppen der Honigbiene (Testgruppen) können in einem Stoffwechseltest zwischen den Gerüchen verwandter und unverwandter Arbeiterinnen unterschieden. Zunächst wird die Testgruppe dem Duft einer Supergeschwistergruppe (Kontrollgruppe) ausgesetzt. Dann wird ihr kurz der Duft einer anderen Arbeiterinnengruppe (Probengruppe) angeboten. Die Test gruppe vergleicht das Kontrol- mit dem Probensignal und erhöht kurzfristig ihren Stoffwechsel korrelierend zur genetischen Verwandtschaft von Kontrolle und Probe. Die Testgruppe benutzt nicht ihren eigen Geruch als Referenz. Werden der Testgruppe die Düfte gemischter Gruppen aus verwandten und unverwandten Bienen als Probe angeboten, so ziegt sie immer eine maximale Reaktion, als wären alle Bienen in der Probengruppe unverwandt. Bereits die Anwesenheit mehr als einer unverwandten Biene in einer Gruppe von 40 Supergeschwistern löst eine maximale Reaktion aus. Dieses Verhalten läßt sich auf der Basis der Erkennung von Nestzugehörigkeit erklären.
    Notes: Summary Groups of honey bee workers (test groups) are able to discriminate between odours of related and unrelated workers in a metabolic bioassay. First, the test group is exposed to the odour of a super sister group of workers (control group). Then they are briefly exposed to the odour of another worker group (sample group) with varying degrees of relatedness to the control group. The oxygen consumption of all three groups is monitored and the test group increases its metabolic activity if it can discriminate between control and sample group. The test group's reaction negatively correates with the genetic relationship between both groups. It does not use its own group odour as a template. If genetically mixed sample groups of related and unrelated bees are used, the test group shows a full response similar to pure unrelated sample groups. The presence of more than one unrelated worker in a sample group of 40 super sisters elicits a maximal metabolic response. This behaviour can be plausibly explained with nestmate recognition.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Insectes sociaux 39 (1992), S. 365-372 
    ISSN: 1420-9098
    Keywords: Apis mellifera ; kin recognition ; trophallaxis ; polyandry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Kin recognition and nepotism between honeybee workers (Apis mellifera L.) was analysed in a trophallactic bio-assay. Donor workers were fed dyed sugar syrup and introduced into a recipient group consisting of 12 to 15 workers of the same colony. After allowing for 1 hour of trophallaxis, the distribution of the dyed food was analysed with spectrophotometry. The subfamily composition in the recipient group was varied such that the donor bees had to discriminate between workers of 2 to 7 different patrilines. Donor bees preferentially fed super sisters if few patrilines were present in the recipient group. However, preferential feeding was not observed if the recipient group consisted of workers of more than three subfamilies. Since the natural degree of polyandry causes intracolonial genetic variance to exceed the genetic variability in the experiments, nepotistic behaviour among workers may not reveal intranidal subfamily recognition in honeybees.
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  • 6
    ISSN: 1420-9098
    Keywords: Key words: Honeybees, absconding, arrhenotoky, thelytoky.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary: We investigated absconding frequency and latency in queenright and queenless honeybee colonies in thelytokous Apis mellifera capensis, arrhenotokous Apis mellifera scutellata and their natural thelytokous hybrids. There was no significant difference in frequency of absconding among any of the queenright colonies. Absconding was significantly greater in thelytokous queenless colonies than in the queenless arrhenotokous ones. Latency to absconding did not differ among the three groups of queenright colonies nor between the queenright and queenless colonies of A.m. capensis and A.m. scutellata. There were significant differences in latency between queenright and queenless hybrids and significant differences in latency among the three groups of queenless colonies. Among queenless colonies, A.m. capensis absconded twice as readily as did A. m. scutellata and the hybrids were intermediate. Afterabsconding events include the fates of the absconding colony as well as nestmates left behind. One group of orphaned nestmates of A. m. capensis amalgamated with another queenright colony. In the case of A. m. scutellata either drones were produced or the residual queenless colony was joined by a queenless thelytokous group, subsequently reared a queen and then absconded. Differences in the rate and degree of ovarial development indicate that queenless thelytokous workers have the physiological capacity for reproduction, a trait that contributes to colony fitness.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Insectes sociaux 41 (1994), S. 263-272 
    ISSN: 1420-9098
    Keywords: Apis mellifera ; mating frequency ; subfamily ; DNA fingerprinting ; intracolonial worker relationship
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The number and frequencies of subfamilies in a honey bee colony were determined by DNA fingerprinting. Queen and brood samples were taken from three colonies with artificially inseminated queens and from one colony with a naturally mated queen. UsingHae III restriction enzyme and (GATA)4 oligonucleotide, the number of subfamilies in the colonies with artificially inseminated queens corresponded with the number of drones used for insemination. In the colony with the naturally mated queen, 12 subfamilies were found in a random sample of 104 workers. Considering that subfamily frequencies range from 1 to 26%, introcolonial worker relationship was estimated to be 0.328, corresponding to a genetical effective number of 6.4 matings.
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  • 8
    ISSN: 1420-9098
    Keywords: Key words: Worker reproduction, mitochondrial DNA, nuclear DNA, genetic diversity, Apis mellifera, hybrid zone.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary: Two subspecies of honeybees, Apis mellifera capensis and A. m. scutellata provide an ideal model to test for the significance of reproductive workers in natural populations of honeybees. Laying workers of A.m.capensis parthenogenetically produce female offspring (thelytoky) whereas workers of the other subspecies produce male offspring (arrhenotoky). By using a two allele marker system in both the mitochondrial (mt) and in the nuclear (nuc) DNA, a deterministic population genetical model shows that through the differences in laying worker reproduction alone, clines of the mt and the nuc marker should be shifted. The stronger the impact of laying workers the further should the capensis mt type introgress into the scutellata population. The theoretical model is supported by empirical data from the hybrid zone between the two subspecies. The nuc hybrid zone begins 200 km south of the mt hybrid zone indicating a significant impact of the laying workers on colony reproduction.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 42 (1986), S. 445-448 
    ISSN: 1420-9071
    Keywords: Apis mellifera ; sperm competition ; relatedness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Queens, homozygous for three marker genes, were inseminated with eight different types of semen, each carrying one combination of the markers. Intracolonial worker relationship (řc) was estimated by genotype frequencies in the offspring of the experimental queens. řc was larger than under random distribution of semen types in the spermatheca. Estimates of most quantitative genetical parameters will be more accurate using the presented estimator for intracolonial relationship. The insemination order affected genotype frequencies in the offspring, suggesting a weak ‘last male advantage’. There was, however, no evidence of intra spermathecal sperm competition.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 49 (1993), S. 337-338 
    ISSN: 1420-9071
    Keywords: Apis mellifera ; fluorescence in situ hybridization ; rDNA ; chromosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract DNA probes containing the repeated rDNA region ofDrosophila melanogaster (coding for e.g. 28S and 18S rRNA) hybridized in situ to distinct regions of two heterologous mitotic chromosomes of the honeybee, identifying the nucleolus organizing regions (NORs). The method allows a rapid establishment of a physical map ofApis mellifera using other DNA probes ofDrosophila. This is the first report on well-defined chromosomal markers in the honeybee.
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