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  • 1
    Electronic Resource
    Electronic Resource
    Spectrophotometric Determination of Ruthenium(III) Using 1-Nitroso-2-Naphthol-3,6-Disulfonic Acid (Disodium Salt) (1965)
    s.l. : American Chemical Society
    Analytical chemistry 37 (1965), S. 739-741 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac60225a028
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  • 2
    Electronic Resource
    Electronic Resource
    Pax-6 origins – implications from the structure of two coral Pax genes (1998)
    Springer
    Development genes and evolution 208 (1998), S. 352-356 
    Details
    ISSN: 1432-041X
    Keywords: Key words Pax-6 ; Coral ; Acropora ; Homeobox ; Paired box
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Vertebrate Pax-6 and its Drosophila homolog eyeless play central roles in eye specification, although it is not clear if this represents the ancestral role of this gene class. As the most ”primitive” animals with true nervous systems, the Cnidaria may be informative in terms of the evolution of the Pax gene family. For this reason we surveyed the Pax gene complement of a representative of the basal cnidarian class (the Anthozoa), the coral Acropora millepora. cDNAs encoding two coral Pax proteins were isolated. Pax-Aam encoded a protein containing only a paired domain, whereas Pax-Cam also contained a homeodomain clearly related to those in the Pax-6 family. The paired domains in both proteins most resembled the vertebrate Pax-2/5/8 class, but shared several distinctive substitutions. As in most Pax-6 homologs and orthologs, an intron was present in the Pax-Cam locus at a position corresponding to residues 46/47 in the homeodomain. We propose a model for evolution of the Pax family, in which the ancestor of all of the vertebrate Pax genes most resembled Pax-6, and arose via fusion of a Pax-Aam-like gene (encoding only a paired domain) with an anteriorly-expressed homeobox gene resembling the paired -like class.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270050191
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  • 3
    Electronic Resource
    Electronic Resource
    Highly repeated DNA sequences in the scleractinian coral genusAcropora: Evaluation of cloned repeats as taxonomic probes (1990)
    Springer
    Marine biology 104 (1990), S. 483-487 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A plasmid clone containing highly repeated DNA sequence fromAcropora formosa was used to probe slot blots of genomic DNA and Southern blots of Taq I digests from other Acroporidae. Homologous repeated DNA sequences were detected in 12 otherAcropora species, but not in three other species of Acroporidae (Astreopora sp.,Montipora digitata andM. aequituberculata). Slot-blot experiments indicated that the repeated sequences inAcropora latistella, A. tenuis, A. longicyathus andA. nobilis were distantly homologous with the clonedA. formosa repeat, whereas those inA. pulchra, A. millepora, A. valida, A. hyacinthus andA. microphthalma were highly homologous with this probe and those inA. digitifera were intermediate. Relatedness of the highhomology group toA. formosa was assessed by comparison of Taq I-digestion patterns. The predominant repeats inA. pulchra andA. hyacinthus had two Taq I sites per repeat unit (as didA. formosa), whereas repeats inA. digitifera, A. valida andA. microphthalma had one Taq I site per repeat; the pattern given byA. millepora implied that its highly homologous repeat units contained either one or two Taq I sites. Within the high-homology group, decreasing number of Taq I sites implies increasing taxonomic distance fromA. formosa. The relatedness series implied by these data differs from that based on morphological criteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314353
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  • 4
    Electronic Resource
    Electronic Resource
    Analysis of ribosomal ITS1 sequences indicates a deep divergence between Rhodactis (Cnidaria: Anthozoa: Corallimorpharia) species from the Caribbean and the Indo-Pacific/Red Sea (1996)
    Springer
    Marine biology 126 (1996), S. 423-432 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper explores the implications of ribosomal ITS1-sequence analysis for evolutionary relationships between four species of corallimorphs which are morphologically very similar but have distinct distributions. The analyses highlight the unreliability of morphological criteria in establishing systematic relationships amongst tropical corallimorphs. Both parsimony and distance methods of phylogenetic analysis strongly support a deep divergence between the Caribbean species Rhodactics sanctithoma and the three morphologically-related species R. howesii and R. indosinensis from the Indo-Pacific and R. rhodostoma from the Red Sea. The analyses also strongly support a closer relationship between the Red Sea species, R. rhodostoma, and one of the Indo-Pacific species, R. howesii, than between these two and the second Indo-Pacific species, R. indosinensis. The first of these results is presumably a consequence of the closure of the Central American Seaway, the event which led to the separate development of Atlantic and Indo-Pacific coral faunas. However, the lack of detailed distribution data represents a major barrier to reconstructing the evolutionary history of the genus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00354624
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and cloning of DNA from somatic tissue of soft corals (Cnidaria: Octocorallia) (1990)
    Springer
    Marine biology 104 (1990), S. 489-492 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is described for isolating high molecular weight DNA from somatic tissue of soft corals. The method avoids problems associated with the presence of nucleases, pigments and other secondary metabolites in soft corals. Tissue is frozen in liquid nitrogen, pulverized with a pestle and mortar, and the powder extracted with buffered sodium dodecyl sulphate at 4°C in the presence of phenol/chloroform. This technique has been applied toAlcyonium, Sinularia, Sarcophyton andLobophytum species. Repeated sequences have been cloned fromAlcyonium sp., and used to probe slot-blots of genomic DNA and Southern blots of restriction digests. Homologous repeated sequences were detected in threeAlcyonium sp., but not in threeSinularia sp., despite these genera being closely related.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314354
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  • 6
    Electronic Resource
    Electronic Resource
    Nucleotide sequencing of highly repetitive DNA from seven species in the coral genusAcropora (Cnidaria: Scleractinia) implies a division contrary to morphological criteria (1991)
    Springer
    Marine biology 110 (1991), S. 323-327 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nucleotide sequences have been determined for 31 homologous 118 base-pair highly repeated DNA sequences from seven species ofAcropora. A matrix was constructed from the sequence data and subjected to phylogenetic analysis using heuristic search routines in the PAUP (phylogenetic analysis using parsimony) program, Version 3.0L. These analyses confirm a close relationship between two species of one subgeneric group (A. pulchra andA. millepora), but identify a division in a group of six species which is contrary to taxonomic groupings based on morphological criteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01344350
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  • 7
    Electronic Resource
    Electronic Resource
    NADP+-dependent glutamate dehydrogenase from Acropora formosa: purification and properties (1987)
    Springer
    Marine biology 95 (1987), S. 559-563 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract As an initial step in our study of nitrogen metabolism in the coral/algal symbiosis we have purified glutamate dehydrogenase (EC 1.4.1.4) to homogeneity from polyp tissue of the staghorn coral Acropora formosa collected from Magnetic Island (North Queensland) in 1985–1986. The purified enzyme had a specific activity of 78 U mg-1. The native enzyme had a relative molecular weight, M r, of 360 000 (±20 000), and appears to be a hexamer with subunits of M r=56000 (±3 000). Like the enzyme from other coelenterates, the coral glutamate dehydrogenase (GDH) was absolutely specific with respect to the coenzyme substrate (NADP+/NADPH), and was insensitive to allosteric regulation by nucleotides; unlike other coelenterate GDHs, the coral enzyme was absorlutely specific for ammonium as amino group donor in the reductive amination reaction, and major differences in kinetic properties were apparent. Linear Michaelis-Menten kinetics were observed for the substrates a-ketoglutarate, NADPH and NADP+, the K m values being 0.93, 0.11 and 0.03 mM, respectively. However glutamate dehydrogenase displayed biphasic kinetics with respect to l-glutamate and ammonium, indicating two apparent K m values (18 and 81 mM for l-glutamate and 9.2 and 416 mM for ammonium). The enzyme also exhibits Scatchard plots, Hill coefficients and cooperativity indices characteristic of enzymes displaying negative cooperativity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393099
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  • 8
    Electronic Resource
    Electronic Resource
    Characterization of two glutamate dehydrogenases from the symbiotic microalga Symbiodinium microadriaticum isolated from the coral Acropora formosa (1988)
    Springer
    Marine biology 97 (1988), S. 427-430 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In view of their possible involvement in ammonium assimilation in the coral/algal symbiosis, we have purified two distinct glutamate dehydrogenase isoenzymes from the symbiotic dinoflagellate Symbiodinium microadriaticum (Freudenthal) extracted from the staghorn coral Acropora formosa collected from Magnetic Island, North Queensland, Australia, in 1986–1987. An NADPH-specific glutamate dehydrogenase (GDH) displayed biphasic kinetics with respect to ammonium as the variable substrate; at low substrate concentrations the apparent K m was below 1 mM, whereas at high substrate concentrations the corresponding value was approximately 200 mM. The NADPH-GDH displayed extremely low activity in the direction of glutamate oxidation; together with the kinetic data this suggests a probable role in ammonium assimilation. A second (NADH-specific) GDH was found to have both amination and deamination activities, and presumably functions in vivo in glutamate oxidation. Kinetic constants are reported for both GDH isoenzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397773
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  • 9
    Electronic Resource
    Electronic Resource
    A Device for In Situ Determination of Geochemical Transport Parameters 2. Biochemical Reactions (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 28 (1990), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Chemical and biological reaction rates are required in estimating migration rates of many reactive contaminants in ground water, and as input data for mathematical models. Reaction rates must be measured, and depend on environmental conditions that are often difficult to duplicate in the laboratory. In situ rate measurement minimizes the difficulties of controlling environmental variables such as temperature, pH, Eh, DO, solution and solid phase composition, and bacterial population.The instrument described in this paper isolates a 1.9 liter portion of an aquifer for in situ biochemical rate measurement. The device has been used successfully to measure the rate of reactions involving inorganic and organic contaminants in sandy aquifers. Two applications of the device, measurement of denitrification rates and rates of biodegradation of benzene, are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1990.tb01720.x
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  • 10
    Electronic Resource
    Electronic Resource
    Properties of sputtered superconducting films of Bi2Sr2CaCu2Ox made by low-temperature in situ growth (1989)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 55 (1989), S. 2135-2137 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Superconducting films of Bi2Sr2CaCu2Ox have been fabricated on single-crystal MgO substrates by a low-temperature in situ process. Using a substrate temperature Ts≈645 °C, metallic films with a superconducting onset of 90–100 K and an extrapolated Tc0=56 K have been obtained. X-ray diffraction shows the films to be c-axis oriented. Electron microscopy reveals that the films are not significantly smoother than films which were post-annealed at 865 °C, and that some segregation into nonsuperconducting phases had occurred. The exact mechanism by which crystallization and superconductivity occurs at such low temperatures is not yet known, but it can be speculated that the surface atoms are less constrained and thus have a smaller energy barrier to overcome in forming a crystal structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.102347
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