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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Mycorrhiza 4 (1993), S. 11-15 
    ISSN: 1432-1890
    Keywords: Afromontane forest ; Epiphytic plants ; Fine root ; Tropics ; Vesicular-arbuscular mycorrhizal fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Roots of 28 species of epiphytic vascular plants were collected on tree trunks and branches at six afromontane forest sites between 1700 and 3300 m above sea level in Bale Mountains National Park, Ethiopia. Seven of the 28 epiphyte species were colonized by vesicular-arbuscular mycorrhizal fungi (VAM). Mycorrhizal colonization only occurred at two of the six sites examined, at 2900 m and 3300 m, but more than one type of VAM endophyte was present in each case. Three facultative epiphytic species were all highly colonized by VAM on the forest floor, whereas roots from epiphytic habitats were weakly colonized. No correlations were found between VAM colonization, fine root diameter and root hair length, but VAM colonization and root hair abundance were negatively correlated. The lack of VAM colonization of potential, epiphytic host species at the majority of the sites examined points to the dispersal of VAM propagules as the factor limiting mycorrhizal colonization of epiphytic habitats. It is suggested that root systems of hemiepiphytic tree species serve as corridors between forest floor and tree trunks through which VAM may spread via hyphal growth.
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  • 2
    ISSN: 1432-1939
    Keywords: Key words Arctic soil ; Allelopathy ; Microbial immobilization ; Plant-microbe interactions ; Soil labile carbon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We measured partitioning of N and P uptake between soil microorganisms and potted Festuca vivipara in soil from a subarctic heath in response to factorial addition of three levels of labile carbon (glucose) combined with two levels of inorganic N and P. The glucose was added to either non-sterilized or sterilized (autoclaved) soils in quantities which were within the range of reported, naturally occurring amounts of C released periodically from the plant canopy. The aims were, firstly, to examine whether the glucose stimulated microbial nutrient uptake to the extent of reducing plant nutrient uptake. This is expected in nutrient-deficient soils if microbes and plants compete for the same nutrients. Secondly, we wanted to test our earlier␣interpretation that growth reduction observed in graminoids after addition of leaf extracts could be caused directly by labile carbon addition, rather than by phytotoxins in the extracts. Addition of high amounts of N did not affect the microbial N pool, whereas high amounts of added P significantly increased the microbial P pool, indicating a luxury P uptake in the microbes. Both plant N and in particular P uptake increased strongly in response to soil sterilization and to addition of extra N or P. The increased␣uptake led to enhanced plant growth when both elements were applied in high amounts, but only led to increased tissue concentrations without growth responses when the nutrients were added separately. Glucose had strong and contrasting effects on plant and microbial N and P uptake. Microbial N and P uptake increased, soil inorganic N and P concentrations were reduced and plant N and P uptake declined when glucose was added. The responses were dose-dependent within the range of 0–450 μg C g−1 soil added to the non-sterilized soil. The opposite responses of plants and microbes showed that plant acquisition of limiting nutrients is dependent on release of nutrients from the soil microbes, which is under strong regulation by the availability and microbial uptake of labile C. Hence, we conclude, firstly, that the microbial populations can compete efficiently with plants for nutrients to an extent of affecting plant growth when the microbial access to labile carbon is high in nutrient deficient soils. We also conclude that reduced growth of plants after addition of leaf extracts to soil can be caused by carbon-induced shifts in nutrient partitioning between plants and microbes, and not necessarily by phytotoxins added with the extracts as suggested by some experiments.
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  • 3
    ISSN: 1432-1939
    Keywords: Arctic/alpine soils ; Benomyl ; Microbial C, N, P ; Nutrient immobilization ; Plant nutrient uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The soil microbial carbon (C), nitrogen (N) and phosphorus (P) pools were quantified in the organic horizon of soils from an arctic/alpine low-altitude heath and a high-altitude fellfield by the fumigation-extraction method before and after factorial addition of sugar, NPK fertilizer and benomyl, a fungicide. In unamended soil, microbial C, N and P made up 3.3–3.6%, 6.1–7.3% and 34.7% of the total soil C, N and P content, respectively. The inorganic extractable N pool was below 0.1% and the inorganic extractable P content slightly less than 1% of the total soil pool sizes. Benomyl addition in spring and summer did not affect microbial C or nutrient content analysed in the autumn. Sugar amendments increased microbial C by 15 and 37% in the two soils, respectively, but did not affect the microbial nutrient content, whereas inorganic N and P either declined significantly or tended to decline. The increased microbial C indicates that the microbial biomass also increased but without a proportional enhancement of N and P uptake. NPK addition did not affect the amount of microbial C but almost doubled the microbial N pool and more than doubled the P pool. A separate study has shown that CO2 evolution increased by more than 50% after sugar amendment and by about 30% after NPK and NK additions to one of the soils. Hence, the microbial biomass did not increase in response to NPK addition, but the microbes immobilized large amounts of the added nutrients and, judging by the increased CO2 evolution, their activity increased. We conclude: (1) that microbial biomass production in these soils is stimulated by labile carbon and that the microbial activity is stimulated by both labile C and by nutrients (N); (2) that the microbial biomass is a strong sink for nutrients and that the microbial community probably can withdraw substantial amounts of nutrients from the inorganic, plant-available pool, at least periodically; (3) that temporary declines in microbial populations are likely to release a flush of inorganic nutrients to the soil, particularly P of which the microbial biomass contained more than one third of the total soil pool; and (4) that the mobilization-immobilization cycles of nutrients coupled to the population dynamics of soil organisms can be a significant regulating factor for the nutrient supply to the primary producers, which are usually strongly nutrient-limited in arctic ecosystems.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 112 (1997), S. 305-313 
    ISSN: 1432-1939
    Keywords: Key words Nutrient limitation ; Microbial immobilization ; Festuca vivipara ; Soil labile carbon ; Allelopathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Biomass production was analysed in Festuca vivipara, grown for 3 months in pots with non-sterilized or sterilized soil after factorial addition of three levels of labile carbon combined with high and low levels of N and P. The soil was a nutrient-poor subarctic heath soil. In the non-sterilized soil plant biomass production increased strongly only in the treatment with high levels of both N and P, which suggests that both nutrients limited plant growth. In the sterilized soil addition of a high level of N without P addition gave almost the same growth response as in the combined NP treatment. This was because of a more than 30-fold increase of inorganic phosphorus in the soil as P was released from the killed microbial biomass after sterilization. Sugar addition reduced plant growth in all treatments. The reduction in plant growth was dose dependent within the range of 0–450 μg C g−1 soil added to the non-sterilized soil, but the response levelled off at 233 μg C g−1 soil in the soil that had been sterilized at the start of the experiment. The plant response, together with observed depletion of soil inorganic N and P, indicated that the microbial biomass immobilized nutrients efficiently and reduced plant growth when extra labile carbon was added. The inhibition of growth was lower, however, in the soil which had been sterilized, probably because of a slow recovery of the microbial populations in it. Two of the nutrient-carbon solutions closely matched the N, P and C concentrations in a solution containing leaf extracts of Cassiope tetragona and Betula tortuosa that had been used previously to test for possible allelopathic effects of compounds in the leaf extracts. These extracts also reduced plant growth. The growth reduction was equally large or larger after nutrient-sugar addition than after addition of leaf extracts in three out of the four possible combinations of species and sterilized or non-sterilized soil. In the fourth case (Betula extract added to sterilized soil), the effect was larger when leaf extract was added than after addition of the nutrient-carbon solution. This could be due to a low rate of microbial degradation of phytotoxic substances in this soil because of a slow recovery of the microbial populations after sterilization. The generally stronger or equal effect of the nutrient-sugar addition compared to the leaf extract addition leads to the conclusion that microbial nutrient immobilization and microbial competition for nutrients increased as a function of labile carbon addition with the extract. Hence, it appears that enhanced microbial activity and microbial nutrient immobilization rather than phytotoxic effects was the primary reasons for the reduced biomass production in F. vivipara even after addition of the leaf extracts.
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  • 5
    ISSN: 1432-1939
    Keywords: Allelopathy ; Betula pubescens ssp ; tortuosa ; Cassiope tetragona ; Empetrum hermaphroditum ; Plant-microbe competition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previous research has shown that plant extracts, e.g. from boreal dwarf shrubs and trees, can cause reduced growth of neighbouring plants: an effect known as allelopathy. To examine whether arctic and subarctic plants could also be affected by leaching of phytochemicals, we added extracts from the commonly occurring arctic dwarf shrubs Cassiope tetragona and Empetrum hermaphroditum, and from mountain birch, Betula pubescens ssp. tortuosa to three graminoid species, Carex bigelowii, Festuca vivipara and Luzula arcuata, grown in previously sterilized or non-sterilized arctic soils. The graminoids in non-sterilized soil grew more slowly than those in sterilized soil. Excised roots of the plants in non-sterilized soil had higher uptake rate of labelled P than those in sterilized soil, demonstrating larger nutrient deficiency. The difference in growth rate was probably caused by higher nutrient availability for plants in soils in which the microbial biomass was killed after soil sterilization. The dwarf shrub extracts contained low amounts of inorganic N and P and medium high amounts of carbohydrates. Betula extracts contained somewhat higher levels of N and much higher levels of P and carbohydrates. Addition of leaf extracts to the strongly nutrient limited graminoids in non-sterilized soil tended to reduce growth, whereas in the less nutrient limited sterilized soil it caused strong growth decline. Furthermore, the N and P uptake by excised roots of plants grown in both types of soil was high if extracts from the dwarf shrubs (with low P and N concentrations) had been added, whereas the P uptake declined but the N uptake increased after addition of the P-rich Betula extract. In contrast to the adverse extract effects on plants, soil microbial respiration and soil fungal biomass (ergosterol) was generally stimulated, most strongly after addition of the Betula extract. Although we cannot exclude the possibility that the reduced plant growth and the concomitant stimulation of microbial activity were caused by phytochemicals, we believe that this was more likely due to labile carbon in the extracts which stimulated microbial biomass and activity. As a result microbial uptake increased, thereby depleting the plant available pool of N and P, or, for the P-rich Betula extract, depleting soil inorganic N alone, to the extent of reducing plant growth. This chain of events is supported by the negative correlation between plant growth and sugar content in the three added extracts, and the positive correlation between microbial activity, fungal biomass production and sugar content, and are known reactions when labile carbon is added to nutrient deficient soils.
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  • 6
    ISSN: 1432-1939
    Keywords: Keywords Arctic and alpine ecosystems ; δ15N of vascular plants ; mosses ; lichens and fungi ; Plant nitrogen uptake ; Soil inorganic ; organic and microbial nitrogen ; Stable isotopes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this study we show that the natural abundance of the nitrogen isotope 15, δ15N, of plants in heath tundra and at the tundra-forest ecocline is closely correlated with the presence and type of mycorrhizal association in the plant roots. A total of 56 vascular plant species, 7 moss species, 2 lichens and 6 species of fungi from four heath and forest tundra sites in Greenland, Siberia and Sweden were analysed for δ15N and N concentration. Roots of vascular plants were examined for mycorrhizal colonization, and the soil organic matter was analysed for δ15N, N concentration and soil inorganic, dissolved organic and microbial N. No arbuscular mycorrhizal (AM) colonizations were found although potential host plants were present in all sites. The dominant species were either ectomycorrhizal (ECM) or ericoid mycorrhizal (ERI). The δ15N of ECM or ERI plants was 3.5–7.7‰ lower than that of non-mycorrhizal (NON) species in three of the four sites. This corresponds to the results in our earlier study of mycorrhiza and plant δ15N which was limited to one heath and one fellfield in N Sweden. Hence, our data suggest that the δ15N pattern: NON/AM plants 〉 ECM plants ≥ ERI plants is a general phenomenon in ecosystems with nutrient-deficient organogenic soils. In the fourth site, a␣birch forest with a lush herb/shrub understorey, the differences between functional groups were considerably smaller, and only the ERI species differed (by 1.1‰) from the NON species. Plants of all functional groups from this site had nearly twice the leaf N concentration as that found in the same species at the other three sites. It is likely that low inorganic N availability is a prerequisite for strong δ15N separation among functional groups. Both ECM roots and fruitbodies were 15N enriched compared to leaves which suggests that the difference in δ15N between plants with different kinds of mycorrhiza could be due to isotopic fractionation at the␣fungal-plant interface. However, differences in δ15N between soil N forms absorbed by the plants could also contribute to the wide differences in plant δ15N found in most heath and forest tundra ecosystems. We hypothesize that during microbial immobilization of soil ammonium the microbial N pool could become 15N-depleted and the remaining, plant-available soil ammonium 15N-enriched. The latter could be a main source of N for NON/AM plants which usually have high δ15N. In contrast, amino acids and other soil organic N compounds presumably are 15N-depleted, similar to plant litter, and ECM and ERI plants with high uptake of these N forms hence have low leaf δ15N. Further indications come from the δ15N of mosses and lichens which was similar to that of ECM plants. Tundra cryptogams (and ECM and ERI plants) have previously been shown to have higher uptake of amino acid than ammonium N; their low δ15N might therefore reflect the δ15N of free amino acids in the soil. The concentration of dissolved organic N was 3–16 times higher than that of inorganic N in the sites. Organic nitrogen could be an important N source for ECM and, in particular, ERI plants in heath and forest tundra ecosystems with low release rate of inorganic N from the soil organic matter.
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  • 7
    ISSN: 1432-1939
    Keywords: Altitude ; Carbon isotopes ; Cassiope tetragona ; Empetrum hermaphroditum ; Global change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract As climatic change might induce ecophysiological changes in plants which affect their long-term performance, we investigated responses in above-ground biomass, δ13C, nitrogen and chlorophyll of two evergreen arctic dwarf shrubs, Cassiope tetragona and Empetrum hermaphroditum, to 5 (biomass, N) or 6 years of shading, nutrient application and air/soil warming at a dwarf shrub dominated tree-line heath (450 m a.s.l) and a high altitude fellfield (1100 m a.s.l.) in Swedish Lapland. Warming enhanced the green biomass (equivalent to the last 3–4 years of leaf production) and the ratio of green to brown biomass of C. tetragona at the fellfield, and diluted the shoot N concentration. Fertilizer application led to higher shoot N concentration and larger green-to-brown biomass ratio at both sites, and fertilizer application and warming generally had an additive effect on the green biomass. We conclude that both warming and increased soil nutrient availability stimulated the growth of C. tetragona at the fellfield whereas at the heath there was a clear increase in production only if enhanced temperature was combined with nutrient application. Across treatments C. tetragona at the fellfield had 0.6‰ higher δ13C and 1.4 mg g-1 more leaf N, and the soil organic matter δ13C was 1.0‰ higher at the fellfield than at the heath. However, an increase in shoot N concentration with altitude does not necessarily lead to higher δ13C as no differences in δ13C were observed when leaf N of the two dwarf shrubs was increased by fertilizer application c. tetragona in non-warmed plots had higher δ13C values than those from warmed plots at the same altitude, which provides the first in situ experimental validation of the theory that temperature partly is responsible for altitudinal trends in plant carbon isotope discrimination. Increased biomass and chlorophyll concentration of C. tetragona in warmed plots points to increased assimilation, at least at the fellfield. As the δ13C-based and, therefore, time-integrated estimate of the ratio of CO2 concentration in the leaf intercellular spaces to that in the atmosphere (C i/C a) also increased, warming probably enhanced the stomatal conductance relatively more than the C assimilation, which may be harmful if climatic change leads to reduced soil moisture content and increased plant competition for water. At both sites C. tetragona and E. hermaphroditum responded to shade by increasing the concentration of shoot N and photosynthetic pigments whereas biomass production (and therefore also net photosynthesis) did not decline. Shade was accompanied by a 0.6–1.3‰ (E. hermaphroditum) or 1.2–2.2‰ (C. tetragona) decrease in δ13C. This could be due to enhanced stomatal conductance with shading, and perhaps to shade reducing the ericoid mycorrhizal uptake of soil organic C, a factor which has been overlooked as an influence on plant δ13C.
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  • 8
    ISSN: 1432-1939
    Keywords: Arctic mycorrhiza ; Legume and lichen N2 fixation ; Nitrate reductase activity ; Plant ; soil and precipitation δ15N ; Stable isotopes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The natural abundance of the nitrogen isotope 15, δ15N, was analysed in leaves of 23 subarctic vascular plant species and two lichens from a tree-line heath at 450 m altitude and a fellfield at 1150 m altitude close to Abisko in N. Sweden, as well as in soil, rain and snow. The aim was to reveal if plant species with different types of mycorrhizal fungi also differ in their use of the various soil N sources. The dwarf shrubs and the shrubs, which in combination formed more than 65% of the total above-ground biomass at both sites, were colonized by ericoid or ectomycorrhizal fungi. Their leaf δ15N was between−8.8 and−5.5‰ at the heath and between−6.1 and −3.3‰ at the fellfield. The leaf δ15N of non- or arbuscular mycorrhizal species was markedly different, ranging from −4.1 to −0.4‰ at the heath, and from −3.4 to+2.2‰ at the fellfield. We conclude that ericoid and ectomycorrhizal dwarf shrubs and shrubs utilize a distinct N source, most likely a fraction of the organic N in fresh litter, and not complexed N in recalcitrant organic matter. The latter is the largest component of soil total N, which had a δ15N of −0.7‰ at the heath and +0.5‰ at the fellfield. Our field-based data thus support earlier controlled-environment studies and studies on the N uptake of excised roots, which have demonstrated protease activity and amino acid uptake by ericoid and ectomycorrhizal tundra species. The leaves of ectomycorrhizal plants had slightly higher δ15N (fellfield) and N concentration than leaves of the ericoids, and Betula nana, Dryas octopetala and Salix spp. also showed NO inf3 sup- reductase activity. These species may depend more on soil inorganic N than the ericoids. The δ15N of non- or arbuscular mycorrhizal species indicates that the δ15N of inorganic N available to these plants was higher than that of average fresh litter, probably due to high microbial immobilization of inorganic N. The δ15N of NH inf4 sup+ -N was +12.3‰ in winter snow and +1.9‰ in summer rain. Precipitation N might be a major contributer in species with poorly developed root systems, e.g. Lycopodium selago. Our results show that coexisting plant species under severe nutrient limitation may tap several different N sources: NH inf4 sup+ , NO inf3 sup- and organic N from the soil, atmospheric N2, and N in precipitation. Ericoid and ectomycorrhizal fungi are of major importance for plant N uptake in tundra ecosystems, and mycorrhizal fungi probably exert a major control on plant δ15N in organic soils.
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  • 9
    ISSN: 1573-5036
    Keywords: arctic ; global warming ; microbial biomass ; nematodes ; soil nutrient cycling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Arctic terrestrial ecosystems are strongly dominated by temperature, and global warming is expected to have a particularly strong impact in high latitudes. The Arctic will therefore be an important region for early detection of global change. In the present study the effects of environmental manipulations simulating climate change on soil microorganisms and nematode populations were investigated. Study sites were a dwarf shrub dominated tree-line heath (450 m a.s.l.) and a high altitude fellfield (1150 m a.s.l.) at Abisko, Swedish Lapland. Soil temperature was enhanced by using passive greenhouses and the impact on soil organisms with and without NPK fertilizer addition was assessed. The nematode community was strongly affected by warming and nutrient application. Population density was twice as high for all treatments at the fellfield as compared to controls. At the heath temperature enhancement with or without fertilizer application also led to a doubling of the population density, whereas fertilization alone caused an increase of about one third. The environmental manipulations resulted in a greater microbial biomass C and active fungal biomass in the heath soil. Increased density was also recorded for bacterial and fungal feeding nematodes at both sites. The results suggest that nematodes have an important impact on microbial biomass and turnover rates in the two subarctic systems. Elevated soil temperature apparently will lead to increased grazing on microorganisms, contributing to enhanced net N and P mineralization rates and plant nutrient availability. However, biodiversity was generally affected negatively by the environmental manipulations. The effects were more severe at the high altitude fellfield indicating that the influence of elevated temperature will be more pronounced in systems already stressed by extreme climatic conditions.
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  • 10
    ISSN: 1573-5036
    Keywords: Acacia spp. ; Eucalyptus globulus ; Glomus intraradices ; G. mosseae ; root nodules ; stable isotope ; tropical tree nursery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The aim of the present study was to investigate with the 15N natural abundance method if inoculation with vesicular-arbuscular mycorrhizal (VAM) fungi affects the N2 fixation of legume trees grown under Ethiopian nursery conditions. A further aim was to test if VAM fungi influence the δ15N values of non-fixing plants. Five different VAM inocula and/or phosphorus fertilizer were applied to the standard, non-sterile nursery soil in which seedlings of four different Acacia species and Eucalyptus globulus were grown. After 3–5 months the acacia shoot dry weights and the fraction of the fine roots which were colonized by VAM fungi was increased by four of the VAM inocula. However, inoculation did not influence the δ15N values of non-nodulated A. nilotica or E. globulus; i.e., roots with low and high VAM colonization did not differ in discrimination against 15N. With E. globulus used as a reference species grown under the same conditions and in the same time period as A. abyssinica, the N2 fixation of A. abyssinica accounted for 5.1 to 46.7% of the seedling N content, dependent on the soil treatment. Deep shade reduced the N2 fixation of A. abyssinica from 36.2 to 5.1%. The lower δ15N values of most VAM inoculated, nodulated acacias indicate that N2 fixation was increased by those VAM fungi which also promoted shoot dry weight. However, VAM inoculation generally did not affect shoot N concentrations or nodule dry weights, and the δ15N values were not correlated with shoot N concentrations. N2 fixation may not be important for the N nutrition and growth of seedlings in nutrient-rich nursery soils, but it is an important seedling property after outplanting in N deficient field soils.
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