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  • 1
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Summary 1. After treatment of native or formalin-fixed tendon fibrils with an ammoniacal silver solution, silver particles are deposited in the D-bands of the fibrils. In the native fibrils these are arranged in one, two or three striae per band, but after formalin fixation they lie in one stria only. 2. No external reducing agent is necessary for the production of the particles. 3. Pretreatment of native fibrils with neutral salt solution or with trypsin has no effect on subsequent silvering. On the other hand, silvering is abolished by treatment with hyaluronidase, citrate buffer or periodate and also by acetylation and bisulphite. 4. Formalin-fixed fibrils show the silvering effect after all these procedures except acetylation or bisulphite treatment. 5. It is postulated that silvering of the D-bands is due to reducing substances which can precipitate silver from ammonical solutions and that formalin influences the process by the production of cross linkages.
    Notes: Zusammenfassung 1. Die Behandlung der nativen und formolfixierten Sehnenfibrillen mit einer ammoniakalischen Silberlösung führt immer zu einer Einlagerung von Silberpartikeln in den D-Teilen der Fibrillen. 2. Bei den nativen Fibrillen liegen die Silberkörner in einem, zwei oder drei Streifen im D-Teil. 3. In den formolfixierten Fibrillen ist das Silber nur in einem Streifen vorhanden. 4. Die Behandlung der nativen und formolfixierten Sehnenfibrillen mit anderen Silbersalzen führt zu keiner Versilberung der Fibrillen. 5. Die Behandlung der nativen Sehnenfibrillen mit neutraler Kochsalzlösung oder Trypsin und anschließender Versilberung führt zu keiner wesentlichen Änderung des Silberbildes. 6. Hyaluronidase-, Citratpuffer- und Perjodateinwirkung auf native Sehnenfibrillen mit anschließender Versilberung führt zu keiner Innenversilberung der D-Teile. 7. Acetylierung und Behandlung mit Bisulfit der nativen Fibrillen und anschließender Versilberung mit ammoniakalischer Silberlösung verhindert eine Innenversilberung der D-Teile. 8. Die formolfixierten Fibrillen zeigen eine Innenversilberung der D-Teile nach einer Vorbehandlung mit einer neutralen Kochsalzlösung, Citratpuffer, Hyaluronidase, Trypsin und Perjodat. Nur die Acetylierung und die Behandlung mit Bisulfit verhindert eine Innenversilberung. 9. Die Innenversilberung der Sehnenfibrillen durch eine ammoniakalische Silberlösung wird weder durch Licht noch durch Chloride oder lichtempfindliche Silbereiweißverbindungen hervorgerufen. 10. Die Versilberung in den D-Teilen wird durch Stoffe in den Fibrillen bewirkt, die Silber aus einer ammoniakalischen Silberlösung ausfällen können. 11. Die reduzierenden Stoffe haben enge Beziehungen zur citratlöslichen Fraktion und sind perjodat- und hyaluronidaseempfindlich. Formalinfixierung beeinflußt diesen Versilberungsmodus durch ein vermehrtes Auftreten von Querbindungen. 12. Die Sonderstellung der ammoniakalischen Silberlösung für die Innenversilberung wird diskutiert. Sie kann stereochemische Gründe haben oder durch die große Beständigkeitskonstante erklärt werden. 13. Das Ausfallen von metallischem Silber in den D-Teilen der Sehnenfibrillen kann nicht mit dem photographischen Prozeß in Verbindung gebracht werden. Das gilt auch für die Bindegewebsversilberung nachGömöri. 14. Die Silberorte in den D-Teilen lassen sich nur teilweise mit den bekannten Querstreifungsbildern nach Osmium- oder Phosphorwolframsäurefixierung in Beziehung setzen.
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Macmillan Magazines Ltd.
    Nature 400 (1999), S. 419-420 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Tabin has proposed a progress-zone model, based on published data, to explain the inhibition of limb morphogenesis by thalidomide. We do not think that his model convincingly explains the main features of thalidomide action. We have previously listed the factors that must be taken into ...
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 211 (1966), S. 1401-1402 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Tissues obtained by biopsy or at necropsy from cases of familial Mediterranean fever, multiple myeloma, idio-pathic primary amyloidosis and experimental leish-maniasis-induced amyloidosis in hamsters were fixed in glutaraldehyde-osmium tetroxide, embedded in 'Vestopal', contrasted with uranyl ...
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 296 (1999), S. 565-573 
    ISSN: 1432-0878
    Keywords: Key words Immunohistology ; Immunoprecipitation ; Integrins ; Organoid culture ; Cartilage matrix ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Integrins are cell-surface receptors that mediate cell attachment to extracellular matrix components. The pericellular matrix in cartilage not only is a mechanical framework, but is also important for chondrocyte differentiation and stabilization of the phenotype. The interaction between chondrocytes and pericellular matrix is mediated, in part, by integrin receptors. We have previously demonstrated the presence of β1-integrins in the cartilage matrix of organoid culture of limb buds from 12-day-old mouse embryos by immunohistological methods. In order to corroborate these findings, we have further investigated the distribution of integrins in the cartilage matrix by immunoelectron microscopy and by immunoprecipitation methods. Cartilage tissue of limb buds of 17-day-old mouse embryos was treated with collagenase and the cell-free and cellular protein-free supernatant was removed and used for immunoprecipitation experiments. Immunoprecipitation with antibodies against β1-, α1-, α3-, and α5β1-integrins and collagen type II, followed by immunoblotting with the same antibodies, demonstrated the presence of these integrins and collagen type II in the supernatant. The integrins found in the cartilage matrix could have been either secreted or shed by the cells. The question as to whether they have a function in the cartilage matrix, such as interlinking, in the matrix organization or in the stabilization of matrix components remains to be elucidated.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 28 (1994), S. 95-124 
    ISSN: 1059-910X
    Keywords: Basement membrane ; Lamina densa ; Lamina lucida ; Lamina fibroreticularis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The aim of this contribution is to summarize our knowledge of the morphology of the basement membrane (BM). The first step in this direction is the attempt to define this term. The BM is composed of the Lamina lucida, densa, and fibroreticularis. Subsequently, the historical development of this term is discussed. Our main interest is, of course, focused on the description of the BM-structure up to the macromolecular level and the special forms of this structure. This is supplemented by discussing its chemical composition and establishing a relationship between morphology and biochemistry. The obtained findings yielded some indications as to the molecular composition of the BM which may serve for the construction of “models.” The composition of the Lamina lucida (L.l.) and the Lamina or Pars fibroreticularis (L.f.) must be discussed separately, since, if present, they show a different and strongly varying structure (L.f.). An important aspect is the function of this extracellular layer which comprises mechanical tasks up to inductive effects. Finally, the concepts of the formation of the BM, especially of the Lamina densa (L.d.), are summarized. It obviously consists of a sequence of individual steps which starts with expression and secretion of the L.d.-components and is followed by an induction of integrin expression. © 1994 Wiley-Liss, Inc.
    Additional Material: 14 Ill.
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