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  • 1
    Electronic Resource
    Electronic Resource
    Energy dependence of the α"1-γ angular correlation in the reaction ^1^2C(α, α"1γ)^1^2C
    Amsterdam : Elsevier
    Nuclear Physics, Section A 121 (1968), S. 422-432 
    Details
    ISSN: 0375-9474
    Keywords: Nuclear reactions
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0375-9474(68)90430-2
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  • 2
    Electronic Resource
    Electronic Resource
    Spectroscopy of Gd^3^+ and Eu^3^+ in the calcite structure
    Amsterdam : Elsevier
    Journal of Solid State Chemistry 99 (1992), S. 78-84 
    Details
    ISSN: 0022-4596
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0022-4596(92)90291-3
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  • 3
    Electronic Resource
    Electronic Resource
    Coupling mechanisms in anionic substrate transport across the inner membrane of rat-liver mitochondria (1970)
    Springer
    Journal of bioenergetics and biomembranes 1 (1970), S. 287-307 
    Details
    ISSN: 1573-6881
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The translocation of Pi, malate, α-oxoglutarate, and citrate across the inner membrane of rat-liver mitochondria has been studied. Investigation on the effect of pH on anionic substrate translocation across the mitochondrial membrane shows that their distribution across the inner membrane can be governed by transmembrane pH difference. However, evidence is presented that the translocation of Pi, but not that of malate, α-oxoglutarate, or citrate can bedirectly coupled to an OH− counterflux (H2PO 4 − −OH− exchange-diffusion). and malate-tricarboxylate exchange-diffusion reactions is directly demonstrated. The study of the effect of uncouplers on the efflux from mitochondria of substrate anions, in the absence of counteranion, and on the anion exchange-diffusions shows that uncouplers act in at least two ways: they promote the efflux of Pi from mitochondria and inhibitdirectly the exchange-diffusion reactions. The kinetics of this inhibition are described. These results are discussed in the light of previous work on the effect of uncouplers on the distribution of substrate anions across the inner membrane of isolated mitochondria. Coupling mechanisms in substrate anion translocation and aspects of the energetics of anion translocation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01516289
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  • 4
    Electronic Resource
    Electronic Resource
    Fluctuations in the enzymatic activity of the human endometrium (1983)
    Springer
    Histochemistry and cell biology 77 (1983), S. 159-170 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cyclic fluctuations were studied in the activity of oxidoreductases playing a role in the major energy metabolic pathways, lysosomal and non-lysosomal hydrolases and some non-enzymatic cytochemical components demonstrable in different developmental physiological or pathophysiological phases of human endometrium. The total scope of the study involved 170 tissues and cytological specimens. The cytological material included microcurettings, aspirates, brush preparations and tissue prints. An evaluation of the usefulness of the application of enzyme cytochemistry to cytological material is included. The most important results were a cyclic fluctuation and a progestagenic controlled increase in the activity of many oxidoreductases, especially the NADPH regenerating enzymes of the pentose phosphate pathway, and of the NADP+ dependent isocitrate dehydrogenase. The histochemical evaluation of the activity of these NADP+ linked enzymes can therefore be recommended for the evaluation of the physiological status of the endometrial cells, especially in patients with infertility problems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00506558
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  • 5
    Electronic Resource
    Electronic Resource
    Histochemical technique for the demonstration of pyruvate kinase activity (1988)
    Springer
    Histochemistry and cell biology 88 (1988), S. 501-504 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We describe an enzyme histochemical multistep technique for the demonstration of pyruvate kinase activity. In this technique, a semipermeable membrane is interposed between the incubation medium and the tissue sections, thus preventing diffusion of the enzyme into the medium during the incubation period. In this histochemical system, phosphoenolpyruvate (PEP) donates its phosphate group to ADP in a reaction catalysed by pyruvate kinase. Next, exogenous and endogenous hexokinase catalyses the reaction between ATP andd-glucose to yieldd-glucose-6-phosphate and ADP. Thed-glucose-6-phosphate is oxidized by exogenous and endogenousd-glucose-6-phosphate dehydrogenase, and concomitantly, the generated electrons are transported via NADP+, phenazine methosulphate and menadione to nitro-BT, which is finally precipitated as formazan. Schurin azide and amytal are included to block electron transfei to cytochromes. The method proved to be of value for the qualitative demonstration of pyruvate kinase activity in tissue sections of kidneys, heart muscle and skeletal arusele. For quantitative studies and for investigating the activity of this enzyme in liver sections, the method cannot be recommended.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00570316
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  • 6
    Electronic Resource
    Electronic Resource
    Chemical and physical processes of radionuclide migration at Yucca Mountain, Nevada (1990)
    Springer
    Journal of radioanalytical and nuclear chemistry 142 (1990), S. 331-347 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The understanding and prediction of radionuclide migration within the geosphere of a high-level radioactive waste repository requires knowledge of chemical and physical processes. In this paper, we present an overview of the investigations being performed at Los Alamos National Laboratory for the site characterization activites of the Yucca Mountain Project. The areas of investigation include: the determination of solubility concentration limits and chemical speciation for fission products and actinides; the collection of sorption data and understanding of sorption mechanisms; and the transport mechanism of advection, diffusion, and dispersion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02039472
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  • 7
    Electronic Resource
    Electronic Resource
    A promoter region that controls basal and elicitor-inducible expression levels of the NADPH:cytochrome P450 reductase gene (Cpr ) from Catharanthus roseus binds nuclear factor GT-1 (1997)
    Springer
    Molecular genetics and genomics 256 (1997), S. 674-681 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsCatharanthus roseus ; NADPH:cytochrome P450 reductase ; Cpr ; Elicitor ; GT-1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract NADPH:cytochrome P450 reductase (CPR) is essential for the activation of cytochrome P450 enzymes, which are involved in a wide variety of metabolic pathways in plants, including those related to defence responses. In the subtropical plant Catharanthus roseus several cytochrome P450 enzymes operate in the biosynthesis of defence-related terpenoid indole alkaloids (TIAs). In agreement with the importance of CPR in defence, Cpr mRNA levels in C. roseus were found to be enhanced by fungal elicitor preparations that also induce TIA biosynthesis and P450 gene expression. Here we describe the isolation of a C. roseus genomic DNA clone covering the 5′ part of the Cpr gene and 1.6-kb of upstream sequences. Mapping of the transcription start site showed the untranslated leader sequence is approximately 280 bp long. To study the control of gene expression by the Cpr promoter, transcriptional fusions between Cpr promoter fragments and the gusA reporter gene were generated and their expression was analyzed in stably transformed tobacco plants. The Cpr promoter fragment extending from −1510 to −8, with respect to the ATG start codon, conferred basal and elicitor-inducible expression on the gusA reporter gene, strongly indicating that the Cpr gene of C. roseus is indeed controlled by this promoter region. Progressive deletion from the 5′ end of the promoter to position −632 had little effect on gusA expression. However, deletion to position −366 resulted in a complete loss of basal activity and largely eliminated elicitor-induced expression, indicating that the region from −632 to −366 contains the main transcription-enhancing cis-regulatory sequences. Electrophoretic mobility shift assays with tobacco nuclear extracts showed that binding sites for nuclear factor GT-1 are redundant in the Cpr promoter, but absent from the downstream part of the leader sequence. The presence of strong GT-1 binding sites in the main enhancer region (−632 to −366), is suggestive of a functional role for this factor in basal expression and elicitor responsiveness of the Cpr promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008617
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  • 8
    Electronic Resource
    Electronic Resource
    HD-Zip proteins of families I and II from rice: interactions and functional properties (2000)
    Springer
    Molecular genetics and genomics 263 (2000), S. 12-21 
    Details
    ISSN: 1617-4623
    Keywords: Key words Rice ; HD-Zip proteins ; One-hybrid screen ; Transcription factor ; Dimerization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Proteins of the closely related homeodomain-leucine zipper (HD-Zip) families I and II in plants are putative transcription factors that interact with similar pseudopalindromic DNA recognition sites. We have previously described the Oshox1 gene from rice, which encodes an HD-Zip II protein. To identify further rice HD-Zip proteins, one-hybrid screens were performed in yeast strains containing a HIS3 reporter gene with upstream HD-Zip recognition sites. This resulted in the isolation of six new cDNAs encoding HD-Zip proteins belonging to family I (Oshox4, -5, -6) or family II (Oshox2, -3, -7). In transient assays, using rice suspension-cultured cells transformed by particle bombardment, we showed previously that Oshox1 can transcriptionally repress the activity of reporter gene constructs with upstream HD-Zip binding sites. Here, we confirm the repression properties of Oshox1 by showing that the repression function can be conferred on a heterologous DNA-binding domain. This portable functional domain (residues 1–155) is located proximal to the HD-Zip domain. In yeast, the same region of the Oshox1 protein was found to confer transcriptional activation instead of repression, pointing to the possibility that cell type-specific factors may determine the functional properties of the Oshox1 protein in rice. Like Oshox1, another HD-Zip family II protein (Oshox3) was also found to function as a transcriptional repressor in rice cells. In contrast, two HD-Zip I family proteins (Oshox4 and -5) appeared to act as activators in both rice and yeast cells. Results of two-hybrid assays and electrophoretic mobility shift assays strongly suggest that all HD-Zip proteins of families I and II can form homodimers and also heterodimers with all HD-Zip proteins of the same family. Heterodimerization across the HD-Zip families I and II apparently does not to occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008671
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  • 9
    Electronic Resource
    Electronic Resource
    A note on separable homogeneous extensions of the Specker group (1973)
    Springer
    Archiv der Mathematik 24 (1973), S. 123-125 
    Details
    ISSN: 1420-8938
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01228186
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  • 10
    Electronic Resource
    Electronic Resource
    A note on the extensions of the infinite cyclic group and realizable groups (1974)
    Springer
    Archiv der Mathematik 25 (1974), S. 113-120 
    Details
    ISSN: 1420-8938
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01238649
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