Publication Date:
2006-04-01
Description:
A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-beta-d-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-beta-d-glucan content in barley grain to a cluster of cellulose synthase-like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3;1,4)-beta-d-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-beta-d-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-beta-d-glucan biosynthesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Burton, Rachel A -- Wilson, Sarah M -- Hrmova, Maria -- Harvey, Andrew J -- Shirley, Neil J -- Medhurst, Anne -- Stone, Bruce A -- Newbigin, Edward J -- Bacic, Antony -- Fincher, Geoffrey B -- New York, N.Y. -- Science. 2006 Mar 31;311(5769):1940-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Australian Centre for Plant Functional Genomics; School of Agriculture, Food, and Wine; University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16574868" target="_blank"〉PubMed〈/a〉
Keywords:
Antibodies, Monoclonal
;
Arabidopsis/chemistry/genetics/metabolism
;
Cell Wall/chemistry
;
*Genes, Plant
;
Genome, Plant
;
Genomics
;
Glucosyltransferases/*genetics/metabolism
;
Hordeum/chemistry/genetics
;
Oryza/enzymology/*genetics
;
Plant Leaves/chemistry
;
Plants, Genetically Modified
;
Quantitative Trait Loci
;
Synteny
;
Transformation, Genetic
;
beta-Glucans/analysis/chemistry/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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