Springer Online Journal Archives 1860-2000
Summary By an application of H−O technique of agglutination with extensive absorption experiments, it has been possible to work out the antigenic differences and interrelations of the three species, Cl. acetobutylicum, Cl. roseum, and Cl. felsineum. It was established first that each is distinguished by its species-specific agglutinogens (H or large-flaking in each case); for convenience, these have been called H1, H2 and H3 in Cl. acetobutylicum, Cl. roseum, and Cl. felsineum respectively. By evidence of simple cross tests and absorptions of both W and O sera it was shown that a common O factor exists in the three organisms. In two cases, Cl. acetobutylicum: Cl. felsineum, and Cl. felsineum: Cl. roseum, the O factor alone accounts for the group reactions. In the third case, Cl. acetobutylicum: Cl. roseum, there is in addition an H factor in the group reaction. On the basis of absorption tests it is believed that Cl. acetobutylicum possesses some H2 (in addition to its species-specific H1). This would allow interaction by the following mechanism: in Cl. roseum-W serum Cl. acetobutylicum reacts because of its possession of some H2; in the Cl. acetobutylicum-W serum Cl. roseum reacts because the H agglutinins of that serum are a complex of anti-H1 (dominant and species-specific) plus anti-H2 (subordinate and group-reacting). No further admixture of H factors between the organisms has been found.
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