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  • 1
    Keywords: bulk water ; climate change ; equitable access ; large dams ; river basins ; water conservation ; water infrastructure ; water legislation ; water management ; water quality ; water resources ; water supply ; water use
    Description / Table of Contents: The past decade has witnessed a major global shift in thinking about water, including the role that water infrastructure plays in sustainable development. This rethinking aims to balance better the social, economic, and environmental performance aspects in the development and management of large dams. Infrastructure strategies must complement strategies for water, environment, and energy security and for emerging concerns to reduce vulnerability in water resource systems to climate change on the horizon. Communication is central to multi-stakeholder dialogue and partnerships at all levels needed to achieve sustainability and governance reform in water resource management and infrastructure provision. At the same time, communication drives the advocacy to mobilize political will and public support for beneficial change and continuous improvement in practices. This case study emphasizes that is not only important to mobilize all opportunities to reconcile water demand and supply in river basins that are coming under increasing levels of water stress, but also to integrate effectively governance and anti-corruption reforms and sustainability improvements into all stages of the planning and project cycle - adding value for stakeholders.
    Pages: Online-Ressource (XIII, 116 Seiten)
    ISBN: 9780821384343
    Language: English
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  • 2
    Publication Date: 2012-04-16
    Description: Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-dependent Cl - channel located in the plasma membrane, and its malfunction results in Cystic Fibrosis (CF), the most common lethal genetic disease in Caucasians. Most CF patients carry the deletion of Phe508 (ΔF508 mutation); this mutation prevents the delivery of the CFTR to its correct cellular location, the apical (lumen-facing) membrane of epithelial cells. Molecular chaperones play a central role in determining the fate of ΔF508-CFTR. In this report we show that the Matrine, a quinolizidine alkaloid, downregulates the expression of the molecular chaperone HSC70 and increases the protein levels of ΔF508-CFTR in human alveolar basal epithelial cells (A549 cell line), stably transfected with a ΔF508-CFTR-expressing construct. Moreover, Matrine induced ΔF508-CFTR release from endoplasmic reticulum to cell cytosol and its localization on the cell membrane. Interestingly, downregulation of HSC70 resulted in increased levels of ΔF508-CFTR complexes with the co-chaperone BAG3, that in addition appeared to co-localize with the mutated protein on the cell surface. These results shed new light on ΔF508-CFTR interactions with proteins of the chaperones/co-chaperones system and could be useful in strategies for future medical treatments for Cystic Fibrosis. J. Cell. Physiol. © 2011 Wiley Periodicals, Inc.
    Electronic ISSN: 1097-4652
    Topics: Biology , Medicine
    Published by Wiley
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  • 3
  • 4
    Publication Date: 2012-05-17
    Print ISSN: 0021-9541
    Electronic ISSN: 1097-4652
    Topics: Biology , Medicine
    Published by Wiley
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  • 5
  • 6
    Publication Date: 2023-07-10
    Keywords: 47-397; Abundance estimate; Amaurolithus amplificus; Amaurolithus delicatus; Amaurolithus primus; Amaurolithus tricorniculatus; Catinaster calyculus; Catinaster coalitus; Ceratolithus acutus; Ceratolithus rugosus; Ceratolithus telesmus; Coccolithus pelagicus; Cyclococcolithus leptoporus; Cyclococcolithus macintyrei; Deep Sea Drilling Project; DEPTH, sediment/rock; Discoaster asymmetricus; Discoaster aulakos; Discoaster bellus; Discoaster berggrenii; Discoaster bollii; Discoaster braarudii; Discoaster brouweri; Discoaster calcaris; Discoaster challengerii; Discoaster decorus; Discoaster deflandrei; Discoaster exilis; Discoaster hamatus; Discoaster icarus; Discoaster intercalaris; Discoaster kugleri; Discoaster loeblichii; Discoaster neohamatus; Discoaster neorectus; Discoaster pentaradiatus; Discoaster perclarus; Discoaster prepentaradiatus; Discoaster pseudovariabilis; Discoaster quinqueramus; Discoaster signus; Discoaster stellulus; Discoaster subsurculus; Discoaster surculus; Discoaster tamalis; Discoaster variabilis; Discolithina japonica; Discolithina multipora; DRILL; Drilling/drill rig; DSDP; DSDP/ODP/IODP sample designation; Glomar Challenger; Helicopontosphaera kamptneri; Leg47; Lithostromation perdurum; Nannofossil abundance; North Atlantic/CONT RISE; Pseudoemiliania lacunosa; Reticulofenestra pseudoumbilicus; Rhabdosphaera procera; Sample code/label; Scyphosphaera apsteinii; Scyphosphaera globulata; Scyphosphaera halldali; Scyphosphaera intermedia; Scyphosphaera procera; Scyphosphaera pulcherrima; Scyphosphaera tubifera; Sphenolithus abies; Sphenolithus neoabies; Sphenolithus sp.; Stratigraphy; Triquetrorhabdulus rugosus
    Type: Dataset
    Format: text/tab-separated-values, 1731 data points
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  • 7
    Publication Date: 2021-07-14
    Description: The 91.15 m thick St. Thomas section belongs to the Middle Globigerina Limestone, which is the intermediate member of the Globigerina Limestone Formation, and crops out along the eastern cliff of the Delimara Peninsula (the south-eastern part ofMalta Island). The sedimentary record is composed of alternating calcareous marls and marly limestones with subordinated prominent bioturbated indurated limestones that are deposited in a pelagic environment. For the first time paleomagnetic analyses of a Burdigalian succession have provided reliable data that allow the identification of five magnetozones that have been correlated to the Astronomically Tuned Neogene Time Scale, spanning the Early Miocene C6n–C5Dn interval. Calcareous plankton quantitative analyses integrated with paleomagnetic analyses, allowed us to identify and date several bioevents that have great potential for Mediterranean and extra-Mediterranean correlations in the interval between ~19.7 and ~17.2 Ma. In particular, the complete distributional range of the calcareous nannofossil Sphenolithus belemnos has been recorded as follows: the First Occurrence (FO) at 19.12 Ma, the First Common Occurrence (FCO) at 19.05 Ma, the Last Common Occurrence (LCO) at 18.44 Ma, and the Last Occurrence (LO) at 18.02 Ma. In addition, the FO and the FCO of Sphenolithus heteromorphus have been documented at 18.29 Ma and at 17.99 Ma, respectively. A new paracme interval in the lower part of the range of this species is described between 17.56 and 17.31 Ma. Concerning the planktonic Foraminifera, the main bioevents are the Common Interval top of Paragloborotalia siakensis at 19.55 Ma, a Common Interval of Globoquadrina dehiscens between 19.34 and 18.48 Ma, the Globigerinoides subquadratus FO at 18.43 Ma, and the onset of an acme interval of Paragloborotalia acrostoma that changes its coiling from random to prevalently sinistral at the same time, at 18.40 Ma. These new biostratigraphic data allowed us to place the succession in the standard Mediterranean calcareous plankton zonal schemes and to make some amendments to these schemes. The FCO of S. belemnos was revealed a more reliable marker than its FO for the base of the MNN3a Zone.With regard to the planktonic Foraminifera, the P. acrostoma AB-r/s has been used as a subzonal marker of the G. dehiscens/Catapsydrax dissimilis Zone, which now comprises three subzones instead of two. The deep marine paleoenvironmental setting, the excellent outcrops and the recognition of a continuous succession of bio-magnetostratigraphic events suggest that the St. Thomas section should be considered as a reference section for the lower Burdigalian of theMediterranean area and for future studies in the definition of the Burdigalian GSSP.
    Description: Published
    Description: 66-89
    Description: 1A. Geomagnetismo e Paleomagnetismo
    Description: JCR Journal
    Description: restricted
    Keywords: Mediterranean; Early Miocene; Calcareous nannofossils; Planktonic Foraminifera; Magnetostratigraphy ; 04. Solid Earth::04.04. Geology::04.04.10. Stratigraphy
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 8
    Publication Date: 2021-09-08
    Description: Results of high-resolution integrated stratigraphic studies (calcareous plankton and magnetostratigraphy) of three Mediterranean sections (La Vedova in Central Italy, Contrada Pesciarello in Sicily and St. Peter’s Pool in Malta) and one deep-sea core from the mid-latitude North Atlantic (DSDP Hole 608) are here synthesized. They are compared with those of previously studied deep marine sections in the Mediterranean including the historical stratotype, with the aim to select the most suitable section and criterion to define the Langhian GSSP (Global Stratotype Section and Point). This study significantly improves the existing magnetobiostratigraphic framework for the upper Burdigalian-lower Langhian and opens new perspectives for defining the Langhian GSSP. An extensive review of the first stages of the Globigerinoides-Praeorbulina evolutionary lineage pointed out that the Praeorbulina datum, historically used (and/or suggested) to approximate the base of the Langhian, coincides with the First Occurrence (FO) of P. glomerosa curva instead of P. sicana which is here included in the genus Globigerinoides. The FO of P. glomerosa curva occurs close to the C5Br/C5Bn.2n reversal boundary. As a consequence, the two recommended guiding criteria to recognize the base of the Langhian, i.e. the Praeorbulina datum and the top of Chron C5Cn.1n, can not be applied together, as these two events differ in age by ~740 kyr. The selection of either of these two criteria will imply a different duration for the Langhian: relatively short, in case the FO of P. glomerosa curva is selected, or longer and probably more acceptable, in case the top of Chron C5Cn.1n is selected. The primary calcareous plankton biohorizons which, according to our data, appoximate the top of Chron C5Cn.1n, are the Last Common Occurrence (LCO) of the calcareous nannofossil Helicosphaera ampliaperta and the LCO of the planktonic foraminifer Paragloborotalia bella. Other calcareous plankton events have been recorded close to the same magnetic reversal, such as a short influx of H. ampliaperta (Ia1) and the Paragloborotalia siakensis Acme0. The P. siakensis Acmea End (AaE) and the Sphenolithus heteromorphus Paracme End (PE) represent the primary plankton biohorizons associated with the P. glomerosa curva FO. Two of the three studied Mediterranean sections (La Vedova and St. Peter’s Pool), each from different point of view, are potentially suitable to be proposed as candidates for the definition of the Langhian GSSP. Yet, further studies on stable isotope stratigraphy and cyclostratigraphy, including astronomical tuning are necessary before the most suitable section and guiding criterion can be selected.
    Description: Published
    Description: xxx-xxx
    Description: 2.2. Laboratorio di paleomagnetismo
    Description: JCR Journal
    Description: reserved
    Keywords: calcareus plankton stratigraphy ; magnetostratigraphy ; Langhian GSSP ; Mediterranean ; mid-latitude North Atlantic Ocean ; 04. Solid Earth::04.04. Geology::04.04.10. Stratigraphy ; 04. Solid Earth::04.05. Geomagnetism::04.05.06. Paleomagnetism
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 9
    ISSN: 1432-1017
    Keywords: Key words Triplex DNA ; FPA ; DNA dynamics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract A synthetic DNA triple helix sequence was formed by annealing a pyrimidinic 21 mer single strand sequence onto the complementary purinic sequence centred on a 27 mer duplex DNA. Melting of the third strand was monitored by UV spectrophotometry in the temperature range 10–90 °C. The Tm of the triplex, 37 °C, was well separated from the onset of duplex melting. When the same triple helix was formed on the duplex bearing one nick in the center of the pyrimidinic sequence the Tm of the triplex was shifted to approximately 32 °C and overlapped the melting of the duplex. We have used fluorescence polarization anisotropy (FPA) measurements of ethidium bromide (EB) intercalated in duplex and triplex samples to determine the hydrodynamic parameters in the temperature range 10–40 °C. The fluorescence lifetime of EB in the samples of double and triple stranded DNA is the same (21.3 ± 0.5 ns) at 20 °C, indicating that the geometries of the intercalation sites are similar. The values for the hydration radii of the duplex, normal triplex, and nicked triplex samples were 10.7 ± 0.2, 12.2 ± 0.2, and 12.0 ± 0.2 Å. FPA measurements on normal triplex DNA as a function of temperature gave a melting profile very similar to that derived by UV absorption spectroscopy. For the triplex carrying a nick, the melting curve obtained using FPA showed a clear shift compared with that obtained for the normal triplex sample. The torsional rigidity of the triplex forms was found to be higher than that of the duplex form.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1017
    Keywords: Key wordsγ-Ray footprinting ; Fluorescence polarization anisotropy ; DNA damage ; Oxidative lesion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The influence of the oxidative lesion 2′-deoxy-7-hydro-8-oxoguanosine (8-oxodG) on some conformational properties of DNA has been studied. Four 30-mer duplexes of the form [5′-GATCCTCTAGAGTC[G* or G]ACCTGCAGGCATGCA-3′]:[3′-CTAGGAGATCTCAG[C or A]TGGACGTCCGTACGT-5′], in which G* is the 8-oxodG lesion, were synthesized in order to compare the effect of the GA mismatch and of the damaged G*C and G*A forms with the normal GC. Spectroscopic measurements performed by means of UV denaturation and circular dichroism experiments do not show gross changes of stability and overall structure in the damaged and mismatched samples. The control DNA and the samples containing GA mismatch show very similar γ-rays cutting patterns, indicating that the introduction of the GA mismatch does not perturb the phosphate backbone geometry. In the samples containing the 8-oxodG there are some variations of the cleavage pattern near G* which are extended for almost one helical turn. Some differences are observed between G*C and G*A duplexes. In particular, in the G*C sample the reduced accessibility to OH radicals at the G15 site, observed in the control, spreads on the intrastrand adjacent bases and in the G*A sample a shift of the minimum is observed. The hydrodynamic radius R h derived by fluorescence polarization anisotropy decay exhibits a constant value of 11.4 ± 0.2 Å between 5 and 40 °C, in all the samples. The torsional constant α of each oligomer decreases when the temperature is raised and the α values of the damaged samples are higher than those of the normal ones.
    Type of Medium: Electronic Resource
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