ISSN:
1040-0397
Keywords:
Cholesterol
;
Polypyrrole
;
Cholesterol oxidase
;
Flow injection analysis
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Biosensors for cholesterol were prepared by electropolymerization of pyrrole in solutions of 0.1 M phosphate buffer containing 10 mM NaClO4 and 15 to 55 IU/mL of cholesterol oxidase at a potential of 800 mV versus a calomel reference electrode (3 M KCl). The sensors were tested for their response toward cholesterol in batch, flow-through, and flow-injection systems by detection of the hydrogen peroxide formed in the enzymatic reaction. Flow-injection analysis (FIA) measurements with an injection loop of 50 μL were performed with a high reproducibility in the range of 0 to 250 μM cholesterol with solutions of constant detergent concentration (1% Triton X-100 in 0.1 M phosphate buffer of pH 7.0) and at a flow rate of 0.2 mL/min. At this flow rate, the detection limit was approximately 5 μM cholesterol and the sample throughput was 25 injections per hour. The response of the sensors toward ascorbic and uric acid was investigated.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140050908
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