ALBERT

Description: Background: Staphylococcus aureus is one of the most important and frequent cause of nosocomial infections worldwide. This study was carried out to investigate the prevalence of Vacomycin-Resistant Staphylococcus aureus and antibiotic sensitivity pattern in clinical isolates in Khartoum. Methods: One hundred and thirty three various samples were collected from some hospitals in Khartoum over a period of 5 months. The samples were cultured on bacteriological media for the isolation of Staphylococcus aureus using standard methods of isolation and identification of bacteria. The Staphylococcus aureus were tested for Methicillin susceptibility using 5 μg Oxacillin disc and Oxacillin E-test, with Resistance defined as an MIC of ≥ 4µg/ ml. Results: In this study all MRSA isolates displayed an Oxacillin MIC of ≥256µg/ml. The MRSA strains were 41.0% while the Resistance to vancomycin was examined by vancomycin E-test, with resistance defined as an MIC of ≥16 µg ml. In this study all VSSA isolates displayed vancomycin MIC of ≤ 2µg/ml "except three intermediate resistant isolate MIC between 4-8 µg/ml". The percentage of the VISA strains was 12.0%. Discussion: The unprevalence of resistance to vancomycin may be related to the low usage of this antibiotic in this study area The majority MRSA isolates were multidrug-resistant (MDR) to different classes of antibiotics including; amoxicillin /clavulinic acid, ampicillin, tetracycline, erythromycin clindamycin and ciprofloxacin. Nitroforantoine exhibit no resistant pattern while 92% of MRSA isolates were susceptible to chloramphenicol. The unprevalence of resistance to Vancomycin may be related to the low usage of this antibiotic so Vancomycin might be used as a drug of choice for the treatment of MRSA infection.