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  • 1
    Electronic Resource
    Electronic Resource
    Phospholipids bound to acidic nuclear proteins in human B and T lymphocytes (1976)
    Springer
    Molecular and cellular biochemistry 12 (1976), S. 67-71 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Chromatin fractions (DNA, histones and nonhistone chromosomal proteins NHCP) have been isolated from human peripheral B and T lymphocytes using different methods and analyzed in order to identify their lipid content. While DNA and histone fractions do not reveal the presence of lipids, a 2% of phospholipids is present in the NHCP fraction. The phospholipids associated with NHCP present a constant relative ratio among sphingomyelin, phosphatidyl-choline and phosphatidyl-ethanolamine both in B and T lymphocytes, whichever are the extraction procedures employed. These findings are related to the possible derepressive role of phospholipids on DNA-dependent RNA synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01731551
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  • 2
    Electronic Resource
    Electronic Resource
    Gradient fractionation of cycling and resting cells monitored by BrdUrd incorporation (1988)
    Springer
    Histochemistry and cell biology 89 (1988), S. 241-245 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A number of techniques are currently employed for the fractionation of heterogeneous cell populations or for the separation of cells in different phases of their cycle. With the development of osmotically inert colloidal silica particles media, density gradient centrifugation became an established method for the separation and purification of cells and subcellular particles. We have applied this technique to the separation of cycling from resting Friend erythroleukemia cells, to obtain purified populations for further biological assays. The flow cytometric analysis of DNA content of the different fractions obtained by the gradient and stained with Propidium lodide (PI), showed the S compartment highly concentrated in the 1.073/77g/ml interface, while the upper levels of the gradient were highly enriched of cells in G1 phase. Moreover, the dual parameter analysis of DNA content by means of Bromodeoxyuridine (BrdUrd) incorporation and PI staining, showed that part of the cells in the 1.067/73 fraction represented the early S phase even if their DNA level, measured on the basis of PI fluorescence was within the diploid cell cluster. This method seems to be suitable to obtain pure cell fractions even when dealing with numerically large populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493147
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of phosphatidylcholine vesicles on the activity of DNA polymerase-α (1979)
    Springer
    Molecular and cellular biochemistry 27 (1979), S. 135-138 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Phosphatidylcholine vesicles stimulate the activity of the DNA polymerase-α from calf thymus. This effect is dependent upon the way of addition of the Mg ions, and the extent of the 3H-dTTP incorporation is closely related to the concentration of the vesicles. A role of phospholipids on the activity of the DNA-related enzymes is suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215361
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  • 4
    Electronic Resource
    Electronic Resource
    Lipid-F1 nucleohistone interactions (1976)
    Springer
    Molecular and cellular biochemistry 10 (1976), S. 153-160 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary High concentrations of phospholipids determine destabilization of F1 histone-DNA complex at the weight ratios, histone: DNA, 0.8:1 and 1:1, but low concentrations cause only negligible destabilization. Cholesterol at high weight ratios has little effect on nucleohistone stability. Only linolenic acid of the fatty acids used reproduces similar changes in the thermal stability of F1 histone-DNA complex as phospholipids. The type of interaction of phospholipids with the F1 histone-DNA complex is analyzed, and the involvement of phospholipids in DNA replicationin vivo is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01731686
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  • 5
    Electronic Resource
    Electronic Resource
    H2B nucleohistone — phospholipid interactions. Thermal denaturation and ultrastructural analysis (1978)
    Springer
    Molecular and cellular biochemistry 20 (1978), S. 159-166 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Sphingomyelin, phosphatidylserine, bovine lecithin and phosphatidylethanolamine modify the thermal stability of H2B-DNA complexes, by inducing stabilization at 0.3 and 0.6 H2B:DNA weight ratios and destabilization at 0.8, 1.0 and 1.5 ones. The ability of phospholipids to modify the arrangement of nucleohistone is confirmed by ultrastructural analysis which indicates a competitive action of these molecules during the nucleoprotein assembly. A possible regulatory role of phospholipids on native chromatin is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00243762
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  • 6
    Electronic Resource
    Electronic Resource
    Lymphocyte clones from old subjects: growing rate and functional activity (1987)
    Springer
    Cytotechnology 1 (1987), S. 103-105 
    Details
    ISSN: 1573-0778
    Keywords: aging ; clone ; lymphocyte ; phenotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Old subjects exhibit a decline in circulating T cells and an impaired proliferative response to mitogens, plus a relative increase in cells with NK phenotype not associated with a concomitant increase in their cytolitic activity. In the present study a limiting dilution assay was used to evaluate the phenotype, the functional activity and the proliferative capacity of clones obtained from peripheral blood lymphocytes of old and young subjects. CD5+ CD8+ clones from old people showed a significant impairment in their proliferative capacity and a decreased lytic activity against K562 and P815-IgG cell lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351134
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  • 7
    Electronic Resource
    Electronic Resource
    Phospholipase C digestion induces the removal of nuclear RNA: A cytochemical quantitative study (1989)
    Springer
    Journal of molecular histology 21 (1989), S. 491-500 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary It has been reported that the incubation of isolated rat liver nuclear matrices with phospholipase C causes the digestion of the matrix-bound phospholipids and the release of most matrix-linked RNAs (Coccoet al., 1980). In this paper, the presence of phospholipids in nuclear substructures and the effects of their removal by phospholipase C digestion have been investigated by means of enzyme—colloidal gold cytochemistry. The nuclear phospholipids appear to be localized in the interchromatin areas and in the nucleolus and are virtually absent in the heterochromatin, when labelled with phospholipase C—colloidal gold. The double labelling test with ribonuclease A and phospholipase C conjugated with gold particles of different diameters shows that the nuclear phospholipids are co-localized with RNA-containing structures. The enzymatic digestion of phospholipids on thin sections of either isolated nuclei or pancreas embedded in LR White resin results in the decrease of the RNase-A colloidal gold labelling of nuclear RNA-containing structures, but not of the rough endoplasmic reticulum. The data confirm the presence of phospholipids in the nucleus in the absence of possible translocation due to isolation procedures and strengthen the hypothesis that they are involved in interactions between nucleic acids and proteins of the nuclear matrix.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01845799
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  • 8
    Electronic Resource
    Electronic Resource
    Chromatin organization in isolated nuclei: Flow cytometric characterization employing forward and perpendicular light scatter (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 6 (1988), S. 31-38 
    Details
    ISSN: 0263-6484
    Keywords: Perpendicular light scatter ; forward light scatter ; chromatin ; flow cytometry ; electron microscopy ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Flow cytometric perpendicular and forward light scatters have been employed to evaluate whether the changes in chromatin organization due to ionic strength, Mg++ concentration and pH, visible in electron microscopy, can be monitored by flow cytometry.The average intensity of the perpendicular light scatter signal increased as nuclear chromatin became decondensed by lowering the ionic strength or releasing H1 histone at low pH values.These results indicate that flow cytometry signals and in particular the perpendicular light scatter allow the detection of the conformational transitions in chromatin and may therefore be useful for studying cell cycle associated morphological changes in isolated nuclei.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290060106
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  • 9
    Electronic Resource
    Electronic Resource
    Inositol lipids in friend erythroleukemia cells: Evidence for changes in nuclear metabolism after differntiation (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 9 (1991), S. 135-145 
    Details
    ISSN: 0263-6484
    Keywords: Inositol lipids ; differentiation ; nucleus ; Friend erythroleukemia cells ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The incorporation of 32Pi into phospholipids was studied in Friend erythroleukemia cells either induced or not to erythroid differentiation with 4 mM hexamethylenebisacetamide (HMBA). The effect of the differentiating agent on the recovery of radiolabelled phospholipids was compared in whole cells, isolated nuclei and nuclear matrix after in vivo labelling for 1 hr. The procedure employed for the isolation of nuclei was demonstrated to allow only negligible lipid redistribution caused by cell manipulations. Among the lipids extractable from nuclei, acidic phospholipids, and particularly polyphosphoinositides, were more represented than in whole cells, while small differences were found in the other phospholipid classes examined. The comparison between the uninduced and induced condition showed that the relative amounts of nuclear inositol lipids were modified by HMBA treatment of the cells, with a decreased recovery of phosphatidylinositol 4,5 bisphosphate.These results indicate that phosphatidylinositol and its phosphorylation products synthesized in vivo show a different metabolism in nuclei and whole cells. They appear to be tightly bound nuclear components, also present in membrane-deprived nuclei and nuclear matrix, and are probably related to the nuclear events involved in erythroid differentiation.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290090211
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  • 10
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    Phospholipids bound to acidic nuclear proteins in human B and T lymphocytes (1976)
    Springer
    Details
    Publication Date: 1976-08-01
    Print ISSN: 0300-8177
    Electronic ISSN: 1573-4919
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Published by Springer
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