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  • 1
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    The radish ( Raphanus sativus L.) mitochondrial cox2 gene contains an ACG at the predicted translation initiation site (1998)
    Springer
    Details
    Publication Date: 1998-08-27
    Print ISSN: 0172-8083
    Electronic ISSN: 1432-0983
    Topics: Biology
    Published by Springer
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    PAPER CURRENT
  • 2
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    Novel mitochondrial genomes in Brassica napus somatic hybrids (1992)
    Springer
    Details
    Publication Date: 1992-09-01
    Print ISSN: 0172-8083
    Electronic ISSN: 1432-0983
    Topics: Biology
    Published by Springer
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    PAPER CURRENT
  • 3
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    Characterization of the radish mitochondrial nad3/rps12 locus: analysis of recombination repeats and RNA editing (1996)
    Springer
    Details
    Publication Date: 1996-05-23
    Print ISSN: 0172-8083
    Electronic ISSN: 1432-0983
    Topics: Biology
    Published by Springer
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    PAPER CURRENT
  • 4
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    Integrin-like proteins are localized to plasma membrane fractions, not plastids, in Arabidopsis (1999)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: Integrins are a large family of integral membrane proteins that function in signal transduction in animal systems. These proteins are conserved in vertebrates, invertebrates, and fungi. Evidence from previous research suggests that integrin-like proteins may be present in plants as well, and that these proteins may function in signal transduction during gravitropism. In past studies, researchers have used monoclonal and polyclonal antibodies to localize beta 1 integrin-like proteins in plants. However, there is a disparity between data collected from these studies, especially since molecular weights obtained from these investigations range from 55-120 kDa for integrin-like proteins. To date, a complete investigation which employs all three basic immunolabeling procedures, immunoblotting, immunofluorescence microscopy, and immunogold labeling, in addition to extensive fractionation and exhaustive controls, has been lacking. In this paper, we demonstrate that use of a polyclonal antibody against the cytoplasmic domain of avian beta 1-integrin can produce potential artifacts in immunolocalization studies. However, these problems can be eliminated through use of starchless mutants or proper specimen preparation prior to electrophoresis. We also show that this antibody, when applied within the described parameters and with careful controls, identifies a large (100 kDa) integrin-like protein that is localized to plasma membrane fractions in Arabidopsis.
    Keywords: Life Sciences (General)
    Type: Plant & cell physiology (ISSN 0032-0781); Volume 40; 2; 173-83
    Format: text
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    NASA TECHNICAL REPORTS
  • 5
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    Immunolocalization of integrin-like proteins in Arabidopsis and Chara (1997)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: Integrins are a large family of integral plasma membrane proteins that link the extracellular matrix to the cytoskeleton in animal cells. As a first step in determining if integrin-like proteins are involved in gravitropic signal transduction pathways, we have used a polyclonal antibody against the chicken beta1 integrin subunit in western blot analyses and immunofluorescence microscopy to gain information on the size and location of these proteins in plants. Several different polypeptides are recognized by the anti-integrin antibody in roots and shoots of Arabidopsis and in the internodal cells and rhizoids of Chara. These cross-reactive polypeptides are associated with cellular membranes, a feature which is consistent with the known location of integrins in animal systems. In immunofluorescence studies of Arabidopsis roots, a strong signal was obtained from labeling integrin-like proteins in root cap cells, and there was little or no immunolabel in other regions of the root tip. While the antibody stained throughout Chara rhizoids, the highest density of immunolabel was at the tip. Thus, in both Arabidopsis roots and Chara rhizoids, the sites of gravity perception/transduction appear to be enriched in integrin-like molecules.
    Keywords: Life Sciences (General)
    Type: Physiologia plantarum (ISSN 0031-9317); 99; 1; 7-14
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
    Electronic Resource
    Electronic Resource
    Characterization of the radish mitochondrial nad3/rps12 locus: analysis of recombination repeats and RNA editing (1996)
    Springer
    Current genetics 29 (1996), S. 564-571 
    Details
    ISSN: 1432-0983
    Keywords: Key words  Gene transfer ; Homologous recombination ; Male sterility ; RNA editing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   In order to further investigate sequences that are responsible for low-frequency recombination in plant mitochondrial DNAs and RNA editing in radish mitochondria, the nad3/rps12 locus has been isolated and characterized from a normal cultivar of radish and the male-sterile Ogura cytoplasm. A repeated sequence that has been implicated in other radish mitochondrial DNA rearrangements was identified at the breakpoint between the two loci indicating that it was also involved in the nad3/rps12 rearrangement. Similar to some other radish mitochondrial genes, nad3/rps12 genomic sequences already contain several, but not all, of the bases that are typically edited in plant mitochondrial nad3 and rps12 genes. Analysis of nad3/rps12 cDNAs indicated that the mRNAs are not edited. One partially edited transcript was identified out of the twenty two that were examined. This finding, along with the observation that nad3/rps12 RNAs are present at very low levels, raises the possibility that radish mitochondria may not encode functional copies of these genes. Consistent with this hypothesis, DNA-blot analysis detects nad3/rps12 sequences in the nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050086
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  • 7
    Electronic Resource
    Electronic Resource
    The radish (Raphanus sativus L.) mitochondrial cox2 gene contains an ACG at the predicted translation initiation site (1998)
    Springer
    Current genetics 34 (1998), S. 79-87 
    Details
    ISSN: 1432-0983
    Keywords: Key words ACG start codon ; cox2 Gene ; Translation ; RNA editing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mitochondrial cox2 gene has been sequenced from radish (Raphanus sativus L.). The gene is interrupted by a 1346-bp group-II intron and contains an ACG codon as the predicted translation initiation site. Analysis of cox2 cDNAs indicates that the ACG codon is not converted to an AUG codon in the mRNA, although 15 other RNA editing sites were identified. The cox2 gene from Raphanus raphanistrum, and other varieties of R. sativus, also contain an ACG as the predicted start codon; plants in the closely related genus, Brassica, do not. Western-blot analyses indicate that cox2 proteins in radish mitochondria are the same size as those found in Brassica mitochondria and different from cox2 proteins in plants where cox2 is nuclear-encoded. This finding, along with the observation that cox2 sequences are not present in the nuclear genome of radish, suggests that ACG is utilized as the radish cox2 initiation codon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050369
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  • 8
    Electronic Resource
    Electronic Resource
    Novel mitochondrial genomes in Brassica napus somatic hybrids (1992)
    Springer
    Current genetics 22 (1992), S. 243-249 
    Details
    ISSN: 1432-0983
    Keywords: Brassica ; Somatic hybrids ; Cytoplasmic male sterility ; Mitochondrial DNA rearrangement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial genomes of nine male-fertile and two Ogura cytoplasmic male-sterile (cms) Brassica napus somatic hybrids were probed with 46 mitochondrial DNA fragments. The distribution of information obtained from each fusion partner was not random. Several regions, including the coxI gene and a major recombination repeat sequence, were always derived from the Brassica campestris fusion partner, and some regions were always derived from the Ogura mitochondrial genome. Novel fragments occurred in seven distinct regions. Some of the rearrangement breakpoints were located near the evolutionary breakpoints relating the mitochondrial genomes of the Brassica species. The sizes of the mitochondrial genomes in the somatic hybrids ranged from 224.8 to 285.3 kb. A direct correlation between a specific gene and the cms phenotype was not observed; however, a possible cms-associated region was identified. It corresponds to a region that was identified through analysis of fertile revertants from a cms B. napus cybrid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351732
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  • 9
    Electronic Resource
    Electronic Resource
    Atrazine-resistant cytoplasmic male-sterile-nigra broccoli obtained by protoplast fusion between cytoplasmic male-sterile Brassica oleracea and atrazine-resistant Brassica campestris (1991)
    Springer
    Theoretical and applied genetics 83 (1991), S. 201-208 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Atrazine resistance ; Cytoplasmic male sterility ; Protoplast fusion ; CMS-nigra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplast fusion was used to combine the cytoplasmic traits of atrazine resistance and male sterility in Brassica oleracea var. italica (broccoli). Leaf protoplasts from broccoli with the petaloid B. nigra type of cytoplasmic male sterility were fused with hypocotyl protoplasts from an atrazine-resistant biotype of B. campestris var. oleifera cv Candle (oilseed rape). A total of 19 colonies regenerated shoots, all of which were broccolilike in phenotype, i.e., lacked trichomes. Four shoots, all from one colony, were atrazine resistant, surviving and growing in the presence of 25 μM atrazine. A leaf piece assay also confirmed that they were atrazine resistant. Molecular analysis showed that they contain chloroplasts from the atrazine-resistant B. campestris parent and mitochondria from the B. nigra parent. No recombination or rearrangement of the mitochondrial genomes in the fusion products was detected. These four plants and their progeny all showed the petaloid B. nigra type of male sterility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226252
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructure of microsporogenesis and microgametogenesis inArabidopsis thaliana (L.) Heynh. ecotype Wassilewskija (Brassicaceae) (1995)
    Springer
    Protoplasma 185 (1995), S. 7-21 
    Details
    ISSN: 1615-6102
    Keywords: Arabidopsis thaliana ; Microgametogenesis ; Microsporogenesis ; Pollen development ; Tapetal cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The process of microsporogenesis and microgametogenesis was studied at the ultrastructural level in wild-typeArabidopsis thaliana ecotype Wassilewskija to provide a basis for comparison with nuclear male-sterile mutants of the same ecotype. From the earliest stage studied to mature pollen just prior to anther dehiscence, microsporocyte/microspore/pollen development follows the general pattern seen in most angiosperms. The tapetum is of the secretory type with loss of the tapetal cell walls beginning at about the time of microsporocyte meiosis. Wall loss exhibits polarity with the tapetal protoplasts becoming located at a distance from the inner tangential walls first, followed by an increase in distance from the radial walls beginning at the interior edge and progressing outward. The inner tangential and radial tapetal walls are completely degenerated by the microspore tetrad stage. Unlike other members of the Brassicaceae that have been studied, the tapetal cells ofA. thaliana Wassilewskija also lose their outer tangential walls, and secretion occurs from all sides of the cells. Exine wall precursors are secreted from the tapetal cells in a process that appears to involve dilation of individual endoplasmic reticulum cisternae that fuse with the tapetal cell membrane and release their contents into the locule. Following completion of the exine, the tapetal cell plastids develop membranebound inclusions with osmiophilic and electron-transparent regions. The plastids undergo ultrastructural changes that suggest breakdown of the inclusion membranes followed by release of their contents into the locule prior to the complete degeneration of the tapetal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01272749
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