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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 51 (1977), S. 199-211 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 11 mit Tritium markierte Aminosäuren wurden jeweils in Glucose-Lösung an Culex-Weibchen verfüttert, die 2–3 Tage zuvor an normalen bzw. mit Plasmodium cathemerium-infizierten Kanarien vögeln gesogen hatten. Die aufgenommene Radioaktivität erscheint stets zuerst im Kropf, der Cardia und dem Mitteldarm. Danach werden die übrigen Organe mehr oder weniger radioaktiv. Die maximal erreichbare Markierung des gesamten Organismus ist anscheinend für jede einzelne Aminosäure charakteristisch. Grundsätzlich werden essentielle Aminosäuren besonders intensiv aufgenommen; Leucin z.B. nicht nur im Darmepithel, sondern auch in Ovar und Fettkörper. Die nicht essentiellen Aminosäuren Alanin, Glutaminsäure und DOPA werden meist nur in Spuren im Darmbereich wiedergefunden. Es fällt auf, daß infizierte Mücken im Gegensatz zu den normalen Phenylalanin wider Erwarten nur wie eine nicht essentielle Aminosäure aufnehmen.
    Notes: Summary Normal and infected Culex females were fed 11 different amino acids. These substances were labeled with tritium and each was given separately in glucose solution. After the uptake the marker appears first in crop, cardia and midgut. Later on the other tissues get labeled more or less. It seems that the labeling follows a characteristic pattern for each amino acid. Essential amino acids are taken up intensively; for example leucine not only in the midgut epithelium but also in the ovary and the fat body. There is nearly no uptake of the non-essential amino acids alanine, glutamic acid and DOPA by the tissues of both groups of females. These amino acids are only seen in the midgut area and not in the other tissues. In infected mosquitos the uptake of the essential acid, phenylalanine, corresponds only to that of a non-essential amino acid.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 78 (1992), S. 56-59 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Experimental simultaneous infections ofAnopheles stephensi (Diptera: Culicidae) withNosema algerae (Microsporida: Nosematidae) andPlasmodium yoelli nigeriensis under standardized laboratory conditions showed partial suppression of the malaria parasite. At 9 days after an infective bloodmeal, the oocysts in the midgut were counted; 12.1%–66.6% of the double-infected mosquitoes exhibited no oocysts, whereas only 4.5%–12% of the control group showed no oocysts. The mean reduction in oocyst numbers under the influence ofNosema was 84.68%. At 14 days after infection withPlasmodium, the amount of sporozoites was examined; their mean reduction in eight experiments was 70%.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 78 (1992), S. 168-171 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract To quantify the effect ofNosema algerae (Microsporida, Nosematidae) on the development ofPlasmodium falciparum inAnopheles stephensi (Diptera, Culicidae), we carried out infection experiments under standardized laboratory conditions. Apart from a mean reduction of 69% in oocyst development, smaller numbers of oocysts and fewer sporozoites were found in theNosema-infected mosquitoes. In addition, nosematosis resulted in higher mortality. The potential role ofNosema algerae as a biological control agent is discussed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 80 (1994), S. 265-266 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Using the polymerase chain reaction (PCR) for species differentiation within the Anopheles gambiae complex, we examined several hundred Cameroonian mosquito specimens. Applying an approved routine protocol for DNA extraction and PCR conditions, apart from the indubitable identification of most of the specimens, we came across ten PCR products that did not correspond in length to any of the hitherto known amplification products of the sibling species. Sequencing experiments showed the ten products to be of identical length (117 bp) and nucleotide sequence. The total sequence of the novel product is included in the PCR product specific for A. melas, which is known to occur in the same collection area as the ten unidentifiable mosquito specimens. On alignment of the novel PCR product sequence and the A. melas one starting at the 3"-end primer annealing site, the last 20 nucleotides of the novel product, reflecting the sequence of the 2nd PCR primer, showed only 60% homology with the then-corresponding A. melas DNA site. Explanations for the occurrence of the unusual PCR products are given and discussed.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 79 (1993), S. 378-384 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Several components were tested for their ability to replace human serum in cultures ofPlasmodium falciparum set up for the development of gametocytes. Besides a serum-free medium, A*I*M*V (Gibco BRL), RPMI medium supplemented with commercially available serum substitutes was used to culture gametocytes. The following substances served as serum replacements: Basal Medium Supplement (Biochrom), Ultroser G (Gibco BRL) and Nutridoma-SR (Boehringer Mannheim). All serum-free additives supported some parasite growth, but only in RPMI supplemented with Nutridoma-SR were morphologically mature gametocytes obtained. The asexual forms developed almost as well as in RPMI with human serum added.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 80 (1994), S. 84-86 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Plasmodium falciparum strains from different malaria-endemic regions were grown in continuous culture without human serum. The medium was complemented with Nutridoma-SR instead of serum. All parasite strains developed well in serum-free medium. Two strains were thawed without serum and showed good multiplication in subsequent continuous culture.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 77 (1991), S. 458-460 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 9
    facet.materialart.
    Unknown
    In:  In: Lozán, J.L.; Graßl, H.; Jendritzky, G.; Karbe, L.; Reise, K. (Hrsg.) Warnsignal Klima: Gesundheitsrisiken. Gefahren für Pflanzen, Tiere und Menschen.Wissenschaftliche Auswertungen, Hamburg, 169-172
    Publication Date: 2008
    Description: Malaria in Europa früher und heute, Verbreitung von Malariavektoren in Europa, Klimaabhängige Entwicklung und Verbreitung der Vektoren und Malariaerreger KATASTER-BESCHREIBUNG: Zunahme sporadisch auftretender Einzelfälle der Malaria durch klimabedingte Temperaturerhöhung nicht auszuschließen, Epidemien oder Reetablierung der Malaria bei gegebenem Standard der Gesundheitsfürsorge höchst unwahrscheinlich KATASTER-DETAIL:
    Keywords: Europa ; Umweltmedizin ; Infektionskrankheiten
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