ISSN:
1432-1211
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Studies on the regulation of a major histocompatibility complex (MHC) class II gene,HLA-DQA1, in Ia-positive cells (Raji, a human B-lymphoma cell line) and in isogenic la-negative cells (RJ2.2.5, a mutant of Raji altered at theAIR-1 locus) are reported. As previously found,AIR-1 is required in its entirety for the activity of an enhancer factor, the absence of which abolishes transcription of MHC class II genes. In this paper, we show thatHLA-DQA1 gene expression can be directed by an enhancerless promoter. The fact that this promoter is inactive in the RJ2.2.5 mutant suggests that thetrans-acting element determined by theAIR-1 locus is not only an enhancer factor as previously described, but also acts at the MHC class II promoter level.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02115012
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