Springer Online Journal Archives 1860-2000
Chemistry and Pharmacology
Abstract Application of either acetylcholine (ACh, 10−5 M) or cholecystokininoctapeptide (CCK-8, 10−8 M) to the isolated rat pancreas elicited large increases in amylase secretion, radiolabelled45Ca2+ influx and cytosolic free calcium [Ca2+]i levels in zero and normal (1.1 mM) extracellular magnesium [Mg2+]o. Elevated [Mg2+]o significantly (p〈0.001) reduced the secretagogueevoked secretory responses and Ca2+ mobilisation. Stimulation of pancreatic segments with either ACh (10−5 and 10−6 M) or CCK-8 (10−8 and 10−10 M) resulted in marked elevation in Mg2+ concentration in effluent samples (net efflux). On removal of either ACh or CCK-8, Mg2+ concentration returned to resting level. In pancreatic acinar cells loaded the flourescent dye magfura, ACh and CCK-8 evoked marked reduction in cytosolic free Mg2+ concentration [Mg2+]i compared to the resting value of 0.82±0.03 mM (n=50) in normal medium in the absence of secretagogues. In elevated [Mg2+]o (10 mM) medium, [Mg2+]i rises to 0.98±0.04 mM (n=6). Addition of CCK-8 led to only a small reduction in [Mg2+ i in elevated [Mg2+]o. In Mg2+ loaded pancreatic acinar cells, Mg2+ is released in a time dependent manner and this efflux of Mg2+ was sensitive to sodium, extracellular amiloride (1 mM), dinitrophenol (10 mM) and lidocaine (1 mM). The results indicate that Mg2+ is acting as an intracellular messenger to regulate the mobilisation of Ca2+ which in turn mediates enzyme secretion.
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