All Library Books, journals and Electronic Records Telegrafenberg

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

  • 1
    ISSN: 1573-5028
    Keywords: carotenoid biosynthesis ; endosperm ; gene ; maize ; phytoene desaturase ; regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To study regulation of the plastid-localized maize carotenoid biosynthetic pathway, a cDNA encoding phytoene desaturase (PDS) was isolated and characterized. The DNA sequence of the maize Pds cDNA was determined and compared with available dicot Pds genes. The deduced PDS protein, estimated at 64.1 kDa (unprocessed), had a dinucleotide binding domain and conserved regions characteristic of other carotene desaturases. Alignment of available PDS sequences from distantly related organisms suggests that Pds has potential as a phylogenetic tool. By use of heterologous complementation in Escherichia coli, maize PDS was shown to catalyze two desaturation steps converting phytoene to ζ-carotene. RFLP (restriction fragment length polymorphism) mapping was used to place Pds on chromosome 1S near viviparous5 (vp5), and RT-PCR (reverse-transcriptase polymerase chain reaction) analysis indicated reduced Pds transcript in vp5 mutant relative to normal endosperm. Other phytoene-accumulating mutant endosperms, vp2 and white3 (w3), showed no difference in Pds transcript accumulation as compared with normal endosperm counterparts. RT-PCR analysis of Pds transcript accumulation in developing endosperm showed Pds was constitutively expressed. Therefore, endosperm carotenogenesis is not regulated by increasing the level of Pds transcripts.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Publication Date: 2018-06-24
    Description: Water, Vol. 10, Pages 832: Water Use Efficiency and Its Influencing Factors in China: Based on the Data Envelopment Analysis (DEA)—Tobit Model Water doi: 10.3390/w10070832 Authors: Shuqiao Wang Li Zhou Hui Wang Xiaocong Li Water resources are important and irreplaceable natural and economic resources. Achieving a balance between economic prosperity and protection of water resource environments is a major issue in China. This article develops a data envelopment analysis (DEA) approach with undesirable outputs by using Seiford’s linear converting method to estimate water use efficiencies for 30 provinces in China, from 2008–2016,and then analyzes the influencing factors while using a DEA-Tobit model. The findings show that the overall water use efficiency of the measured Chinese provinces, when considering sewage emissions as the undesirable output, is 0.582. Thus, most regions still need improvement. Provinces with the highest water efficiency are located in economically developed Eastern China. The spatial pattern of water use efficiency in China is consistent with the general pattern of regional economic development. This study implies that factors like export dependence, technical progress, and educational value have a positive influence on water use efficiency. Further, while industrial structure has had a negative impact, government intervention has had little impact on water use efficiency. These research results will provide a scientific basis for the government to make plans for water resource development, and it may be helpful in improving regional sustainable development.
    Electronic ISSN: 2073-4441
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by MDPI Publishing
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...